Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced Laminitis
Bovine laminitis leads to huge economic losses and animal welfare problems in the dairy industry worldwide. Numerous studies suggested that several metalloproteinases (MPs) may play vital roles in the failure of epidermal attachment. To the best of our knowledge, the present study is the first to in...
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Frontiers Media S.A.
2020-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.597827/full |
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| author | Jiafeng Ding Jiafeng Ding Mingxian Shi Mingxian Shi Long Wang Long Wang Dongdong Qi Dongdong Qi Ze Tao Ze Tao Muhammad A. Hayat Muhammad A. Hayat Tao Liu Tao Liu Jian-tao Zhang Jian-tao Zhang Hongbin Wang Hongbin Wang |
| author_facet | Jiafeng Ding Jiafeng Ding Mingxian Shi Mingxian Shi Long Wang Long Wang Dongdong Qi Dongdong Qi Ze Tao Ze Tao Muhammad A. Hayat Muhammad A. Hayat Tao Liu Tao Liu Jian-tao Zhang Jian-tao Zhang Hongbin Wang Hongbin Wang |
| author_sort | Jiafeng Ding |
| collection | DOAJ |
| description | Bovine laminitis leads to huge economic losses and animal welfare problems in the dairy industry worldwide. Numerous studies suggested that several metalloproteinases (MPs) may play vital roles in the failure of epidermal attachment. To the best of our knowledge, the present study is the first to investigate and characterize the gene-level changes in distinct MPs and endogenous inhibitors using oligofructose (OF)-induced bovine laminitis model. The objective of this study was to determine aberrant MPs and related inhibitors of bovine laminitis in gene level, and to provide reasonable directions for the further protein-level research. Twelve normal Chinese Holstein dairy heifers were randomly divided into treatment group (n = 6) and control group (n = 6). The heifers in the treatment group were administered with OF solutions at a dose of 17 g/kg of body weight via a stomach tube. The heifers were then humanely euthanized when they met the criteria of bovine laminitis. The heifers in the control group were administered with deionized water at a dose of 2 L/100 kg of body weight. They humanely euthanized at 72 h. The gene expressions of MPs and endogenous inhibitors, namely, matrix metalloproteinases (MMPs), A disintegrin and metalloproteinases (ADAMs), and A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTs), and tissue inhibitors of metalloproteinases (TIMPs) in the lamellae from two groups were determined via real-time quantitative PCR. The gene expressions of MMP-2, MMP-9, ADAMTS-4, and ADAMTS-5 significantly increased (P < 0.05), whereas that of TIMP-2 significantly decreased (P < 0.05) in the treatment group relative to the control group. No significant difference was found in the gene expressions of ADAM-10, ADAM-17, TIMP-1, and TIMP-3. These results indicated that the gene-level imbalanced condition of MPs and their TIMPs may be the basic cause for the failure of epidermal attachment. At the same time, more detailed protein-level studies would be needed to further clarify the roles of MPs and TIMPs in the pathogenesis of bovine laminitis, especially to MMP-2, MMP-9, ADAMTS-4, ADAMTS-5, TIMP-2 as well as related substrates (e.g., aggrecan and versican). |
| first_indexed | 2024-12-16T18:41:31Z |
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| id | doaj.art-63edd8253e3a4066b69c736d76563f01 |
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| last_indexed | 2024-12-16T18:41:31Z |
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| spelling | doaj.art-63edd8253e3a4066b69c736d76563f012022-12-21T22:21:02ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-12-01710.3389/fvets.2020.597827597827Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced LaminitisJiafeng Ding0Jiafeng Ding1Mingxian Shi2Mingxian Shi3Long Wang4Long Wang5Dongdong Qi6Dongdong Qi7Ze Tao8Ze Tao9Muhammad A. Hayat10Muhammad A. Hayat11Tao Liu12Tao Liu13Jian-tao Zhang14Jian-tao Zhang15Hongbin Wang16Hongbin Wang17Department of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaDepartment of Veterinary Surgery, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, ChinaBovine laminitis leads to huge economic losses and animal welfare problems in the dairy industry worldwide. Numerous studies suggested that several metalloproteinases (MPs) may play vital roles in the failure of epidermal attachment. To the best of our knowledge, the present study is the first to investigate and characterize the gene-level changes in distinct MPs and endogenous inhibitors using oligofructose (OF)-induced bovine laminitis model. The objective of this study was to determine aberrant MPs and related inhibitors of bovine laminitis in gene level, and to provide reasonable directions for the further protein-level research. Twelve normal Chinese Holstein dairy heifers were randomly divided into treatment group (n = 6) and control group (n = 6). The heifers in the treatment group were administered with OF solutions at a dose of 17 g/kg of body weight via a stomach tube. The heifers were then humanely euthanized when they met the criteria of bovine laminitis. The heifers in the control group were administered with deionized water at a dose of 2 L/100 kg of body weight. They humanely euthanized at 72 h. The gene expressions of MPs and endogenous inhibitors, namely, matrix metalloproteinases (MMPs), A disintegrin and metalloproteinases (ADAMs), and A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTs), and tissue inhibitors of metalloproteinases (TIMPs) in the lamellae from two groups were determined via real-time quantitative PCR. The gene expressions of MMP-2, MMP-9, ADAMTS-4, and ADAMTS-5 significantly increased (P < 0.05), whereas that of TIMP-2 significantly decreased (P < 0.05) in the treatment group relative to the control group. No significant difference was found in the gene expressions of ADAM-10, ADAM-17, TIMP-1, and TIMP-3. These results indicated that the gene-level imbalanced condition of MPs and their TIMPs may be the basic cause for the failure of epidermal attachment. At the same time, more detailed protein-level studies would be needed to further clarify the roles of MPs and TIMPs in the pathogenesis of bovine laminitis, especially to MMP-2, MMP-9, ADAMTS-4, ADAMTS-5, TIMP-2 as well as related substrates (e.g., aggrecan and versican).https://www.frontiersin.org/articles/10.3389/fvets.2020.597827/fullbovine laminibovine laminitispathogenesismetalloproteinaseTIMPreal-time quantitative PCRoligofructose overload model |
| spellingShingle | Jiafeng Ding Jiafeng Ding Mingxian Shi Mingxian Shi Long Wang Long Wang Dongdong Qi Dongdong Qi Ze Tao Ze Tao Muhammad A. Hayat Muhammad A. Hayat Tao Liu Tao Liu Jian-tao Zhang Jian-tao Zhang Hongbin Wang Hongbin Wang Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced Laminitis Frontiers in Veterinary Science bovine laminibovine laminitis pathogenesis metalloproteinase TIMP real-time quantitative PCR oligofructose overload model |
| title | Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced Laminitis |
| title_full | Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced Laminitis |
| title_fullStr | Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced Laminitis |
| title_full_unstemmed | Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced Laminitis |
| title_short | Gene Expression of Metalloproteinases and Endogenous Inhibitors in the Lamellae of Dairy Heifers With Oligofructose-Induced Laminitis |
| title_sort | gene expression of metalloproteinases and endogenous inhibitors in the lamellae of dairy heifers with oligofructose induced laminitis |
| topic | bovine laminibovine laminitis pathogenesis metalloproteinase TIMP real-time quantitative PCR oligofructose overload model |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2020.597827/full |
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