cDNA cloning and mRNA expression of cat and dog Cdkal1
Ichiro Yamamoto, Shingo Ishikawa, Li Gebin, Hiroshi Takemitsu, Megumi Fujiwara, Nobuko Mori, Yutaka Hatano, Tomoko Suzuki, Akihiro Mori, Nobuhiro Nakao, Koh Kawasumi, Toshinori Sako, Toshiro AraiLaboratory of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Tokyo, JapanAbstrac...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2012-08-01
|
| Series: | Veterinary Medicine: Research and Reports |
| Online Access: | http://www.dovepress.com/cdna-cloning-and-mrna-expression-of-cat-and-dog-cdkal1-a10681 |
| _version_ | 1818980623008661504 |
|---|---|
| author | Sako T Arai T Kawasumi K Mori A Nakao N Suzuki T Hatano Y Mori N Fujiwara M Takemitsu H Gebin L Ishikawa S Yamamoto I |
| author_facet | Sako T Arai T Kawasumi K Mori A Nakao N Suzuki T Hatano Y Mori N Fujiwara M Takemitsu H Gebin L Ishikawa S Yamamoto I |
| author_sort | Sako T |
| collection | DOAJ |
| description | Ichiro Yamamoto, Shingo Ishikawa, Li Gebin, Hiroshi Takemitsu, Megumi Fujiwara, Nobuko Mori, Yutaka Hatano, Tomoko Suzuki, Akihiro Mori, Nobuhiro Nakao, Koh Kawasumi, Toshinori Sako, Toshiro AraiLaboratory of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Tokyo, JapanAbstract: The cyclin-dependent kinase 5 regulatory subunit–associated protein 1–like 1 (CDKAL1) gene encodes methylthiotransferase, and the gene contains risk variants for type 2 diabetes in humans. In this study, we performed complementary DNA cloning for Cdkal1 in the cat and dog and characterized the tissue expression profiles of its messenger RNA. Cat and dog Cdkal1 complementary DNA encoded 576 and 578 amino acids, showing very high sequence homology to mammalian CDKAL1 (>88.4%). Real-time polymerase chain reaction analyses revealed that Cdkal1 messenger RNA is highly expressed in smooth muscle and that tissue distribution of Cdkal1 is similar in cats and dogs. Genotyping analysis of single-nucleotide polymorphism for cat Cdkal1 revealed that obese cats had different tendencies from normal cats. These findings suggest that the cat and dog Cdkal1 gene is highly conserved among mammals and that cat Cdkal1 may be a candidate marker for genetic diagnosis of obesity.Keywords: cat, dog, Cdkal1, obese, cDNA cloning, Q-PCR |
| first_indexed | 2024-12-20T17:18:22Z |
| format | Article |
| id | doaj.art-63f1b7ec3341452bb9da1523e410ac69 |
| institution | Directory Open Access Journal |
| issn | 2230-2034 |
| language | English |
| last_indexed | 2024-12-20T17:18:22Z |
| publishDate | 2012-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Veterinary Medicine: Research and Reports |
| spelling | doaj.art-63f1b7ec3341452bb9da1523e410ac692022-12-21T19:31:56ZengDove Medical PressVeterinary Medicine: Research and Reports2230-20342012-08-012012default6569cDNA cloning and mRNA expression of cat and dog Cdkal1Sako TArai TKawasumi KMori ANakao NSuzuki THatano YMori NFujiwara MTakemitsu HGebin LIshikawa SYamamoto IIchiro Yamamoto, Shingo Ishikawa, Li Gebin, Hiroshi Takemitsu, Megumi Fujiwara, Nobuko Mori, Yutaka Hatano, Tomoko Suzuki, Akihiro Mori, Nobuhiro Nakao, Koh Kawasumi, Toshinori Sako, Toshiro AraiLaboratory of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Tokyo, JapanAbstract: The cyclin-dependent kinase 5 regulatory subunit–associated protein 1–like 1 (CDKAL1) gene encodes methylthiotransferase, and the gene contains risk variants for type 2 diabetes in humans. In this study, we performed complementary DNA cloning for Cdkal1 in the cat and dog and characterized the tissue expression profiles of its messenger RNA. Cat and dog Cdkal1 complementary DNA encoded 576 and 578 amino acids, showing very high sequence homology to mammalian CDKAL1 (>88.4%). Real-time polymerase chain reaction analyses revealed that Cdkal1 messenger RNA is highly expressed in smooth muscle and that tissue distribution of Cdkal1 is similar in cats and dogs. Genotyping analysis of single-nucleotide polymorphism for cat Cdkal1 revealed that obese cats had different tendencies from normal cats. These findings suggest that the cat and dog Cdkal1 gene is highly conserved among mammals and that cat Cdkal1 may be a candidate marker for genetic diagnosis of obesity.Keywords: cat, dog, Cdkal1, obese, cDNA cloning, Q-PCRhttp://www.dovepress.com/cdna-cloning-and-mrna-expression-of-cat-and-dog-cdkal1-a10681 |
| spellingShingle | Sako T Arai T Kawasumi K Mori A Nakao N Suzuki T Hatano Y Mori N Fujiwara M Takemitsu H Gebin L Ishikawa S Yamamoto I cDNA cloning and mRNA expression of cat and dog Cdkal1 Veterinary Medicine: Research and Reports |
| title | cDNA cloning and mRNA expression of cat and dog Cdkal1 |
| title_full | cDNA cloning and mRNA expression of cat and dog Cdkal1 |
| title_fullStr | cDNA cloning and mRNA expression of cat and dog Cdkal1 |
| title_full_unstemmed | cDNA cloning and mRNA expression of cat and dog Cdkal1 |
| title_short | cDNA cloning and mRNA expression of cat and dog Cdkal1 |
| title_sort | cdna cloning and mrna expression of cat and dog cdkal1 |
| url | http://www.dovepress.com/cdna-cloning-and-mrna-expression-of-cat-and-dog-cdkal1-a10681 |
| work_keys_str_mv | AT sakot cdnacloningandmrnaexpressionofcatanddogcdkal1 AT arait cdnacloningandmrnaexpressionofcatanddogcdkal1 AT kawasumik cdnacloningandmrnaexpressionofcatanddogcdkal1 AT moria cdnacloningandmrnaexpressionofcatanddogcdkal1 AT nakaon cdnacloningandmrnaexpressionofcatanddogcdkal1 AT suzukit cdnacloningandmrnaexpressionofcatanddogcdkal1 AT hatanoy cdnacloningandmrnaexpressionofcatanddogcdkal1 AT morin cdnacloningandmrnaexpressionofcatanddogcdkal1 AT fujiwaram cdnacloningandmrnaexpressionofcatanddogcdkal1 AT takemitsuh cdnacloningandmrnaexpressionofcatanddogcdkal1 AT gebinl cdnacloningandmrnaexpressionofcatanddogcdkal1 AT ishikawas cdnacloningandmrnaexpressionofcatanddogcdkal1 AT yamamotoi cdnacloningandmrnaexpressionofcatanddogcdkal1 |