Surveying Mutation of FLT3 Genes in Children with Acute Leukemia
Background and purpose: Mutation of FMS like tyrosine kinase (flt3) gene causes uncontrolled proliferation of leukemic cells and a bad prognosis. The present study is aimed at implementing molecular tests to diagnose and screen the mutations in acute leukemia patients. Methodology: Totally, 91 chil...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2017-02-01
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| Series: | Asian Pacific Journal of Cancer Care |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcc/article/view/11 |
| _version_ | 1797344881680580608 |
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| author | Maryam Sheikhi Farhad Zaker Gholamreza Javadi Mehrdad Hashemi Farnaz Razmkhah Abolfazl Movafagh |
| author_facet | Maryam Sheikhi Farhad Zaker Gholamreza Javadi Mehrdad Hashemi Farnaz Razmkhah Abolfazl Movafagh |
| author_sort | Maryam Sheikhi |
| collection | DOAJ |
| description | Background and purpose: Mutation of FMS like tyrosine kinase (flt3) gene causes uncontrolled proliferation of leukemic cells and a bad prognosis. The present study is aimed at implementing molecular tests to diagnose and screen the mutations in acute leukemia patients.
Methodology: Totally, 91 children with acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) were examined as to flt3 mutation, internal tandem duplication (ITD) mutation, and point mutation in exon 17 (e17). ITD mutation in flt3 receptor was carried out in exon 12&12 and intron 11. As to point mutation in e17, PCR products of the subjects after PCR on genomic DNA of them were examined using restriction enzyme (ECORV) and restriction fragment length polymorphism (RFLP). As to ITD positive, sequencing method was used.
Findings: ITD mutation was observed in seven (7.7%) of acute leukemia patients and two (2.2%) patients were diagnosed with point mutation D835. Distribution in different subgroups of FAB was not identical.
Conclusion: FLT3 mutation was highly prevalent in children with acute leukemia. Therefore, molecular diagnosis of these mutation, regardless of FAB categorization and before initiation of intervention, can be used to make better decision about therapeutic protocol. |
| first_indexed | 2024-03-08T11:09:20Z |
| format | Article |
| id | doaj.art-63f4b8fdaaa440039b8c5940c39f3bab |
| institution | Directory Open Access Journal |
| issn | 2588-3682 |
| language | English |
| last_indexed | 2024-03-08T11:09:20Z |
| publishDate | 2017-02-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Care |
| spelling | doaj.art-63f4b8fdaaa440039b8c5940c39f3bab2024-01-26T13:44:49ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Care2588-36822017-02-01217710.31557/apjcc.2017.2.1.711Surveying Mutation of FLT3 Genes in Children with Acute LeukemiaMaryam Sheikhi0Farhad Zaker1Gholamreza Javadi2Mehrdad Hashemi3Farnaz Razmkhah4Abolfazl MovafaghDepartment of Molecular Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.Department of Hematology, Iran University of Medical Science, Medical Science, Tehran.Department of Molecular Genetics,Tehran Medical Sciences Branch, Islamic Azad University, Tehran Iran.1Department of Molecular Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.Department of Molecular Genetics,Tehran Medical Sciences Branch, Islamic Azad University, Tehran Iran.Background and purpose: Mutation of FMS like tyrosine kinase (flt3) gene causes uncontrolled proliferation of leukemic cells and a bad prognosis. The present study is aimed at implementing molecular tests to diagnose and screen the mutations in acute leukemia patients. Methodology: Totally, 91 children with acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) were examined as to flt3 mutation, internal tandem duplication (ITD) mutation, and point mutation in exon 17 (e17). ITD mutation in flt3 receptor was carried out in exon 12&12 and intron 11. As to point mutation in e17, PCR products of the subjects after PCR on genomic DNA of them were examined using restriction enzyme (ECORV) and restriction fragment length polymorphism (RFLP). As to ITD positive, sequencing method was used. Findings: ITD mutation was observed in seven (7.7%) of acute leukemia patients and two (2.2%) patients were diagnosed with point mutation D835. Distribution in different subgroups of FAB was not identical. Conclusion: FLT3 mutation was highly prevalent in children with acute leukemia. Therefore, molecular diagnosis of these mutation, regardless of FAB categorization and before initiation of intervention, can be used to make better decision about therapeutic protocol.http://www.waocp.com/journal/index.php/apjcc/article/view/11acute leukemia, flt4 gene, itd mutation, point mutation d835 |
| spellingShingle | Maryam Sheikhi Farhad Zaker Gholamreza Javadi Mehrdad Hashemi Farnaz Razmkhah Abolfazl Movafagh Surveying Mutation of FLT3 Genes in Children with Acute Leukemia Asian Pacific Journal of Cancer Care acute leukemia, flt4 gene, itd mutation, point mutation d835 |
| title | Surveying Mutation of FLT3 Genes in Children with Acute Leukemia |
| title_full | Surveying Mutation of FLT3 Genes in Children with Acute Leukemia |
| title_fullStr | Surveying Mutation of FLT3 Genes in Children with Acute Leukemia |
| title_full_unstemmed | Surveying Mutation of FLT3 Genes in Children with Acute Leukemia |
| title_short | Surveying Mutation of FLT3 Genes in Children with Acute Leukemia |
| title_sort | surveying mutation of flt3 genes in children with acute leukemia |
| topic | acute leukemia, flt4 gene, itd mutation, point mutation d835 |
| url | http://www.waocp.com/journal/index.php/apjcc/article/view/11 |
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