Bioassay for total serum bioactivity of Atractylodes lancea
The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=1;spage=51;epage=55;aulast=Na-Bangchang |
| _version_ | 1797904843374854144 |
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| author | Kesara Na-Bangchang Anurak Cheoymang Nadda Muhamad Inthuon Kulma |
| author_facet | Kesara Na-Bangchang Anurak Cheoymang Nadda Muhamad Inthuon Kulma |
| author_sort | Kesara Na-Bangchang |
| collection | DOAJ |
| description | The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/ml) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL. |
| first_indexed | 2024-04-10T09:56:35Z |
| format | Article |
| id | doaj.art-63f53f714e8748158ca5e481067c5e04 |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-04-10T09:56:35Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-63f53f714e8748158ca5e481067c5e042023-02-16T12:25:43ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942023-01-01141515510.4103/japtr.japtr_431_22Bioassay for total serum bioactivity of Atractylodes lanceaKesara Na-BangchangAnurak CheoymangNadda MuhamadInthuon KulmaThe study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/ml) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL.http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=1;spage=51;epage=55;aulast=Na-Bangchangatractylodes lanceaatractylodinbioassaycholangiocarcinomapharmacokinetics staphylococcus aureus atcc 25923 |
| spellingShingle | Kesara Na-Bangchang Anurak Cheoymang Nadda Muhamad Inthuon Kulma Bioassay for total serum bioactivity of Atractylodes lancea Journal of Advanced Pharmaceutical Technology & Research atractylodes lancea atractylodin bioassay cholangiocarcinoma pharmacokinetics staphylococcus aureus atcc 25923 |
| title | Bioassay for total serum bioactivity of Atractylodes lancea |
| title_full | Bioassay for total serum bioactivity of Atractylodes lancea |
| title_fullStr | Bioassay for total serum bioactivity of Atractylodes lancea |
| title_full_unstemmed | Bioassay for total serum bioactivity of Atractylodes lancea |
| title_short | Bioassay for total serum bioactivity of Atractylodes lancea |
| title_sort | bioassay for total serum bioactivity of atractylodes lancea |
| topic | atractylodes lancea atractylodin bioassay cholangiocarcinoma pharmacokinetics staphylococcus aureus atcc 25923 |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2023;volume=14;issue=1;spage=51;epage=55;aulast=Na-Bangchang |
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