Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts
Ion doping has rendered mesoporous structures important materials in the field of tissue engineering, as apart from drug carriers, they can additionally serve as regenerative materials. The purpose of the present study was the synthesis, characterization and evaluation of the effect of artemisinin (...
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MDPI AG
2021-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/9/2189 |
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| author | Ioannis Tsamesidis Dimitrios Gkiliopoulos Georgia K. Pouroutzidou Evgenia Lymperaki Chrysanthi Papoulia Karine Reybier Pierre Perio Konstantinos M. Paraskevopoulos Eleana Kontonasaki Anna Theocharidou |
| author_facet | Ioannis Tsamesidis Dimitrios Gkiliopoulos Georgia K. Pouroutzidou Evgenia Lymperaki Chrysanthi Papoulia Karine Reybier Pierre Perio Konstantinos M. Paraskevopoulos Eleana Kontonasaki Anna Theocharidou |
| author_sort | Ioannis Tsamesidis |
| collection | DOAJ |
| description | Ion doping has rendered mesoporous structures important materials in the field of tissue engineering, as apart from drug carriers, they can additionally serve as regenerative materials. The purpose of the present study was the synthesis, characterization and evaluation of the effect of artemisinin (ART)-loaded cerium-doped mesoporous calcium silicate nanopowders (NPs) on the hemocompatibility and cell proliferation of human periodontal ligament fibroblasts (hPDLFs). Mesoporous NPs were synthesized in a basic environment via a surfactant assisted cooperative self-assembly process and were characterized using Scanning Electron Microscopy (SEM), X-ray Fluorescence Spectroscopy (XRF), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction Analysis (XRD) and N2 Porosimetry. The loading capacity of NPs was evaluated using Ultrahigh Performance Liquid Chromatography/High resolution Mass Spectrometry (UHPLC/HRMS). Their biocompatibility was evaluated with the MTT assay, and the analysis of reactive oxygen species was performed using the cell-permeable ROS-sensitive probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA). The synthesized NPs presented a mesoporous structure with a surface area ranging from 1312 m<sup>2</sup>/g for undoped silica to 495 m<sup>2</sup>/g for the Ce-doped NPs, excellent bioactivity after a 1-day immersion in c-SBF, hemocompatibility and a high loading capacity (around 80%). They presented ROS scavenging properties, and both the unloaded and ART-loaded NPs significantly promoted cell proliferation even at high concentrations of NPs (125 μg/mL). The ART-loaded Ce-doped NPs with the highest amount of cerium slightly restricted cell proliferation after 7 days of culture, but the difference was not significant compared with the control untreated cells. |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T07:23:11Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
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| series | Nanomaterials |
| spelling | doaj.art-63f8bd35775148c2a903780edc4d7cc42023-11-22T14:28:40ZengMDPI AGNanomaterials2079-49912021-08-01119218910.3390/nano11092189Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament FibroblastsIoannis Tsamesidis0Dimitrios Gkiliopoulos1Georgia K. Pouroutzidou2Evgenia Lymperaki3Chrysanthi Papoulia4Karine Reybier5Pierre Perio6Konstantinos M. Paraskevopoulos7Eleana Kontonasaki8Anna Theocharidou9Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceLaboratory of Chemical and Environmental Technology, School of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceLaboratory of Advanced Materials and Devices (AMDeLab), School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Biomedical Sciences, International Hellenic University, GR-57001 Nea Moudania, GreeceLaboratory of Advanced Materials and Devices (AMDeLab), School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreecePharmaDev, UMR 152, Université de Toulouse, IRD, UPS, 31000 Toulouse, FrancePharmaDev, UMR 152, Université de Toulouse, IRD, UPS, 31000 Toulouse, FranceLaboratory of Advanced Materials and Devices (AMDeLab), School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceDepartment of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, GreeceIon doping has rendered mesoporous structures important materials in the field of tissue engineering, as apart from drug carriers, they can additionally serve as regenerative materials. The purpose of the present study was the synthesis, characterization and evaluation of the effect of artemisinin (ART)-loaded cerium-doped mesoporous calcium silicate nanopowders (NPs) on the hemocompatibility and cell proliferation of human periodontal ligament fibroblasts (hPDLFs). Mesoporous NPs were synthesized in a basic environment via a surfactant assisted cooperative self-assembly process and were characterized using Scanning Electron Microscopy (SEM), X-ray Fluorescence Spectroscopy (XRF), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction Analysis (XRD) and N2 Porosimetry. The loading capacity of NPs was evaluated using Ultrahigh Performance Liquid Chromatography/High resolution Mass Spectrometry (UHPLC/HRMS). Their biocompatibility was evaluated with the MTT assay, and the analysis of reactive oxygen species was performed using the cell-permeable ROS-sensitive probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA). The synthesized NPs presented a mesoporous structure with a surface area ranging from 1312 m<sup>2</sup>/g for undoped silica to 495 m<sup>2</sup>/g for the Ce-doped NPs, excellent bioactivity after a 1-day immersion in c-SBF, hemocompatibility and a high loading capacity (around 80%). They presented ROS scavenging properties, and both the unloaded and ART-loaded NPs significantly promoted cell proliferation even at high concentrations of NPs (125 μg/mL). The ART-loaded Ce-doped NPs with the highest amount of cerium slightly restricted cell proliferation after 7 days of culture, but the difference was not significant compared with the control untreated cells.https://www.mdpi.com/2079-4991/11/9/2189mesoporous nanopowderscerium dopingartemisinin loadinghemocompatibilityhuman periodontal ligament fibroblasts |
| spellingShingle | Ioannis Tsamesidis Dimitrios Gkiliopoulos Georgia K. Pouroutzidou Evgenia Lymperaki Chrysanthi Papoulia Karine Reybier Pierre Perio Konstantinos M. Paraskevopoulos Eleana Kontonasaki Anna Theocharidou Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts Nanomaterials mesoporous nanopowders cerium doping artemisinin loading hemocompatibility human periodontal ligament fibroblasts |
| title | Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts |
| title_full | Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts |
| title_fullStr | Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts |
| title_full_unstemmed | Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts |
| title_short | Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts |
| title_sort | effect of artemisinin loaded mesoporous cerium doped calcium silicate nanopowder on cell proliferation of human periodontal ligament fibroblasts |
| topic | mesoporous nanopowders cerium doping artemisinin loading hemocompatibility human periodontal ligament fibroblasts |
| url | https://www.mdpi.com/2079-4991/11/9/2189 |
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