Crystal structure of mimivirus uracil-DNA glycosylase.

Cytosine deamination induced by stresses or enzymatic catalysis converts deoxycytidine into deoxyuridine, thereby introducing a G to A mutation after DNA replication. Base-excision repair to correct uracil to cytosine is initiated by uracil-DNA glycosylase (UDG), which recognizes and eliminates urac...

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Main Authors: Eunju Kwon, Deepak Pathak, Hyeun Wook Chang, Dong Young Kim
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5538708?pdf=render
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author Eunju Kwon
Deepak Pathak
Hyeun Wook Chang
Dong Young Kim
author_facet Eunju Kwon
Deepak Pathak
Hyeun Wook Chang
Dong Young Kim
author_sort Eunju Kwon
collection DOAJ
description Cytosine deamination induced by stresses or enzymatic catalysis converts deoxycytidine into deoxyuridine, thereby introducing a G to A mutation after DNA replication. Base-excision repair to correct uracil to cytosine is initiated by uracil-DNA glycosylase (UDG), which recognizes and eliminates uracil from DNA. Mimivirus, one of the largest known viruses, also encodes a distinctive UDG gene containing a long N-terminal domain (N-domain; residues 1-130) and a motif-I (residues 327-343), in addition to the canonical catalytic domain of family I UDGs (also called UNGs). To understand the structural and functional features of the additional segments, we have determined the crystal structure of UNG from Acanthamoeba polyphaga mimivirus (mvUNG). In the crystal structure of mvUNG, residues 95-130 in the N-domain bind to a hydrophobic groove in the catalytic domain, and motif-I forms a short β-sheet with a positively charged surface near the active site. Circular dichroism spectra showed that residues 1-94 are in a random coil conformation. Deletion of the three additional fragments reduced the activity and thermal stability, compared to full-length mvUNG. The results suggested that the mvUNG N-domain and motif-I are required for its structural and functional integrity.
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spelling doaj.art-63fa552315c54584bc5bd74a6f3d90f12022-12-21T19:27:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018238210.1371/journal.pone.0182382Crystal structure of mimivirus uracil-DNA glycosylase.Eunju KwonDeepak PathakHyeun Wook ChangDong Young KimCytosine deamination induced by stresses or enzymatic catalysis converts deoxycytidine into deoxyuridine, thereby introducing a G to A mutation after DNA replication. Base-excision repair to correct uracil to cytosine is initiated by uracil-DNA glycosylase (UDG), which recognizes and eliminates uracil from DNA. Mimivirus, one of the largest known viruses, also encodes a distinctive UDG gene containing a long N-terminal domain (N-domain; residues 1-130) and a motif-I (residues 327-343), in addition to the canonical catalytic domain of family I UDGs (also called UNGs). To understand the structural and functional features of the additional segments, we have determined the crystal structure of UNG from Acanthamoeba polyphaga mimivirus (mvUNG). In the crystal structure of mvUNG, residues 95-130 in the N-domain bind to a hydrophobic groove in the catalytic domain, and motif-I forms a short β-sheet with a positively charged surface near the active site. Circular dichroism spectra showed that residues 1-94 are in a random coil conformation. Deletion of the three additional fragments reduced the activity and thermal stability, compared to full-length mvUNG. The results suggested that the mvUNG N-domain and motif-I are required for its structural and functional integrity.http://europepmc.org/articles/PMC5538708?pdf=render
spellingShingle Eunju Kwon
Deepak Pathak
Hyeun Wook Chang
Dong Young Kim
Crystal structure of mimivirus uracil-DNA glycosylase.
PLoS ONE
title Crystal structure of mimivirus uracil-DNA glycosylase.
title_full Crystal structure of mimivirus uracil-DNA glycosylase.
title_fullStr Crystal structure of mimivirus uracil-DNA glycosylase.
title_full_unstemmed Crystal structure of mimivirus uracil-DNA glycosylase.
title_short Crystal structure of mimivirus uracil-DNA glycosylase.
title_sort crystal structure of mimivirus uracil dna glycosylase
url http://europepmc.org/articles/PMC5538708?pdf=render
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AT hyeunwookchang crystalstructureofmimivirusuracildnaglycosylase
AT dongyoungkim crystalstructureofmimivirusuracildnaglycosylase