Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran
Introduction: Type 2 diabetes mellitus is a complex heterogeneous group of metabolic conditions characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The TCF7L2 gene is considered as one of the major genes susceptible to diabetes.The single...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Ilam University of Medical Sciences
2019-03-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| Subjects: | |
| Online Access: | http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-4100-1&slc_lang=en&sid=1 |
| Summary: | Introduction: Type 2 diabetes mellitus is a complex heterogeneous group of metabolic conditions characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The TCF7L2 gene is considered as one of the major genes susceptible to diabetes.The single nucleotide polymorphism rs7903146 is located in intron 3 of this gene, which is significantly effective in type 2 diabetes.
Materials and methods: In this case-control study, 141 patients and 86 healthy subjects were present. 5cc peripheral blood of diabetics was collected in EDTA-containing tubes. Genomic DNA was extracted using salting-out method and polymorphism genotype was investigated using PCR-RFLP method.
Findings: Genotype and allele frequencies of the rs7903146 polymorphism differed significantly between type 2 diabetic patients and non-diabetic subjects (P = 0.0468 and P = 0.0474 , respectively). The frequency of T allele was 54.5% in type 2 diabetes group and 39.5% in non-diabetic subjects, and this allele was significantly associated with type 2 diabetes risk (OR = 1.8333, 95% CI 1.0456 – 3.2144).
Discussion & Conclusion: Our results confirmed the association between the rs7903146 polymorphism of the TCF7L2 gene and the increased risk of type 2 diabetes in the west-Mazandaran, Iran. |
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| ISSN: | 1563-4728 2588-3135 |