Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran
Introduction: Type 2 diabetes mellitus is a complex heterogeneous group of metabolic conditions characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The TCF7L2 gene is considered as one of the major genes susceptible to diabetes.The single...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2019-03-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-4100-1&slc_lang=en&sid=1 |
| _version_ | 1828475291535671296 |
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| author | Mohammad Shekarzadeh Abas Mohammad poor Mehdi Abasi ROSHAN Mehran Rajabi sahneh saraie Mostafa Daryaie |
| author_facet | Mohammad Shekarzadeh Abas Mohammad poor Mehdi Abasi ROSHAN Mehran Rajabi sahneh saraie Mostafa Daryaie |
| author_sort | Mohammad Shekarzadeh |
| collection | DOAJ |
| description | Introduction: Type 2 diabetes mellitus is a complex heterogeneous group of metabolic conditions characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The TCF7L2 gene is considered as one of the major genes susceptible to diabetes.The single nucleotide polymorphism rs7903146 is located in intron 3 of this gene, which is significantly effective in type 2 diabetes.
Materials and methods: In this case-control study, 141 patients and 86 healthy subjects were present. 5cc peripheral blood of diabetics was collected in EDTA-containing tubes. Genomic DNA was extracted using salting-out method and polymorphism genotype was investigated using PCR-RFLP method.
Findings: Genotype and allele frequencies of the rs7903146 polymorphism differed significantly between type 2 diabetic patients and non-diabetic subjects (P = 0.0468 and P = 0.0474 , respectively). The frequency of T allele was 54.5% in type 2 diabetes group and 39.5% in non-diabetic subjects, and this allele was significantly associated with type 2 diabetes risk (OR = 1.8333, 95% CI 1.0456 – 3.2144).
Discussion & Conclusion: Our results confirmed the association between the rs7903146 polymorphism of the TCF7L2 gene and the increased risk of type 2 diabetes in the west-Mazandaran, Iran. |
| first_indexed | 2024-12-11T06:11:44Z |
| format | Article |
| id | doaj.art-63fcb7a3fe3f426da396ae96ce6f7530 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-12-11T06:11:44Z |
| publishDate | 2019-03-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-63fcb7a3fe3f426da396ae96ce6f75302022-12-22T01:18:07ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352019-03-01266156163Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran MazandaranMohammad Shekarzadeh0Abas Mohammad poor1Mehdi Abasi ROSHAN2Mehran Rajabi sahneh saraie3Mostafa Daryaie4 Dept of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Dept of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Dept of Genetics, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran Dept of Genetics, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran Dept of Genetics, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran Introduction: Type 2 diabetes mellitus is a complex heterogeneous group of metabolic conditions characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The TCF7L2 gene is considered as one of the major genes susceptible to diabetes.The single nucleotide polymorphism rs7903146 is located in intron 3 of this gene, which is significantly effective in type 2 diabetes. Materials and methods: In this case-control study, 141 patients and 86 healthy subjects were present. 5cc peripheral blood of diabetics was collected in EDTA-containing tubes. Genomic DNA was extracted using salting-out method and polymorphism genotype was investigated using PCR-RFLP method. Findings: Genotype and allele frequencies of the rs7903146 polymorphism differed significantly between type 2 diabetic patients and non-diabetic subjects (P = 0.0468 and P = 0.0474 , respectively). The frequency of T allele was 54.5% in type 2 diabetes group and 39.5% in non-diabetic subjects, and this allele was significantly associated with type 2 diabetes risk (OR = 1.8333, 95% CI 1.0456 – 3.2144). Discussion & Conclusion: Our results confirmed the association between the rs7903146 polymorphism of the TCF7L2 gene and the increased risk of type 2 diabetes in the west-Mazandaran, Iran.http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-4100-1&slc_lang=en&sid=1Type 2 diabetes mellitusrs7903146 polymorphismTCF7L2 genePCR-RFLP |
| spellingShingle | Mohammad Shekarzadeh Abas Mohammad poor Mehdi Abasi ROSHAN Mehran Rajabi sahneh saraie Mostafa Daryaie Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām Type 2 diabetes mellitus rs7903146 polymorphism TCF7L2 gene PCR-RFLP |
| title | Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran |
| title_full | Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran |
| title_fullStr | Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran |
| title_full_unstemmed | Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran |
| title_short | Investigating the distribution of TCF7L2 (rs7903146) gene polymorphism in type 2 diabetic patients in West of Iran Mazandaran |
| title_sort | investigating the distribution of tcf7l2 rs7903146 gene polymorphism in type 2 diabetic patients in west of iran mazandaran |
| topic | Type 2 diabetes mellitus rs7903146 polymorphism TCF7L2 gene PCR-RFLP |
| url | http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-4100-1&slc_lang=en&sid=1 |
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