Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease
We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/7/217 |
| _version_ | 1797527528410185728 |
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| author | Dingdong Li Rachel Odessey Dongfang Li Domenico Pacifici |
| author_facet | Dingdong Li Rachel Odessey Dongfang Li Domenico Pacifici |
| author_sort | Dingdong Li |
| collection | DOAJ |
| description | We report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization scheme to design, fabricate, and assess a biochemical sensing platform based on plasmonic interferometry that is able to detect physiological concentrations of TREM2 in solution. These findings open up opportunities for label-free biosensing of TREM2 in its soluble form in various bodily fluids as an early indicator of the onset of clinical dementia in AD. We also show that plasmonic interferometry can be a powerful tool to monitor and optimize surface immobilization schemes, which could be applied to develop other relevant antibody tests. |
| first_indexed | 2024-03-10T09:45:00Z |
| format | Article |
| id | doaj.art-63fd11e1aebc4578a24111c4c436e020 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T09:45:00Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-63fd11e1aebc4578a24111c4c436e0202023-11-22T03:19:42ZengMDPI AGBiosensors2079-63742021-07-0111721710.3390/bios11070217Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s DiseaseDingdong Li0Rachel Odessey1Dongfang Li2Domenico Pacifici3School of Engineering, Brown University, 184 Hope St, Providence, RI 02912, USASchool of Engineering, Brown University, 184 Hope St, Providence, RI 02912, USASchool of Engineering, Brown University, 184 Hope St, Providence, RI 02912, USASchool of Engineering, Brown University, 184 Hope St, Providence, RI 02912, USAWe report an effective surface immobilization protocol for capture of Triggering Receptor Expressed on Myeloid Cells 2 (TREM2), a receptor whose elevated concentration in cerebrospinal fluid has recently been associated with Alzheimer’s disease (AD). We employ the proposed surface functionalization scheme to design, fabricate, and assess a biochemical sensing platform based on plasmonic interferometry that is able to detect physiological concentrations of TREM2 in solution. These findings open up opportunities for label-free biosensing of TREM2 in its soluble form in various bodily fluids as an early indicator of the onset of clinical dementia in AD. We also show that plasmonic interferometry can be a powerful tool to monitor and optimize surface immobilization schemes, which could be applied to develop other relevant antibody tests.https://www.mdpi.com/2079-6374/11/7/217TREM2 sensorsAlzheimer’s diseaseplasmonic interferometryoptical biosensorsurface functionalization |
| spellingShingle | Dingdong Li Rachel Odessey Dongfang Li Domenico Pacifici Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease Biosensors TREM2 sensors Alzheimer’s disease plasmonic interferometry optical biosensor surface functionalization |
| title | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
| title_full | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
| title_fullStr | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
| title_full_unstemmed | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
| title_short | Plasmonic Interferometers as TREM2 Sensors for Alzheimer’s Disease |
| title_sort | plasmonic interferometers as trem2 sensors for alzheimer s disease |
| topic | TREM2 sensors Alzheimer’s disease plasmonic interferometry optical biosensor surface functionalization |
| url | https://www.mdpi.com/2079-6374/11/7/217 |
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