Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype
Abstract Objectives Rabbit adipose mesenchymal stem cells were used for the purpose of studying acquisition of the chondrogenic phenotype over time at 1, 14 and 28 days after in vitro incubation with differentiation media, using nano-liquid chromatography electrospray ionization tandem mass spectrom...
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3750-6 |
| _version_ | 1828345470406098944 |
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| author | Jeffrey R. Janus Stephen G. Voss Benjamin J. Madden Mary Cristine Charlesworth Michael S. Oldenburg Dale Ekbom Serban San-Marina |
| author_facet | Jeffrey R. Janus Stephen G. Voss Benjamin J. Madden Mary Cristine Charlesworth Michael S. Oldenburg Dale Ekbom Serban San-Marina |
| author_sort | Jeffrey R. Janus |
| collection | DOAJ |
| description | Abstract Objectives Rabbit adipose mesenchymal stem cells were used for the purpose of studying acquisition of the chondrogenic phenotype over time at 1, 14 and 28 days after in vitro incubation with differentiation media, using nano-liquid chromatography electrospray ionization tandem mass spectrometry analysis. This was part of a preliminary study of the behavior of differentiated adipose stem cells for use in a rabbit model of laryngoplasty. Data description The data comprise .MGF, .RAW, .MZID, and .XLSX, lists of peaks, peptides and proteins identified by nano-flow liquid chromatography electrospray ionization tandem mass spectrometry analysis upon incubation with non-differentiating (ND) or chondrogenic differentiating (CHD) media (ProteomeXchange ID PXD010236). XLSX files contain the following information: day 1 CT (control, N = 3499 proteins), day 14 ND (N = 3106 proteins), day 28 ND (N = 3116 proteins), day 14 CHD (N = 2901 proteins), and day 28 CHD (N = 2876 proteins). Proteins are characterized with respect to their − 10lgP value, percent coverage, number of total as well as unique peptides after trypsin digestion, derivatization method (carbamidomethylation, oxidation, or combined carbamidomethylation + oxidation), average mass, and include a full description. |
| first_indexed | 2024-04-14T00:12:19Z |
| format | Article |
| id | doaj.art-640099763f1a49eaaa1265892fe9aa7c |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-04-14T00:12:19Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-640099763f1a49eaaa1265892fe9aa7c2022-12-22T02:23:17ZengBMCBMC Research Notes1756-05002018-09-011111310.1186/s13104-018-3750-6Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotypeJeffrey R. Janus0Stephen G. Voss1Benjamin J. Madden2Mary Cristine Charlesworth3Michael S. Oldenburg4Dale Ekbom5Serban San-Marina6Department of Otorhinolaryngology, Mayo ClinicDepartment of Otorhinolaryngology, Mayo ClinicMedical Genome Facility-Proteomics, Mayo ClinicMedical Genome Facility-Proteomics, Mayo ClinicOtolaryngology-Head and Neck Surgery, Prevea Health ServicesDepartment of Otorhinolaryngology, Mayo ClinicDepartment of Otorhinolaryngology, Mayo ClinicAbstract Objectives Rabbit adipose mesenchymal stem cells were used for the purpose of studying acquisition of the chondrogenic phenotype over time at 1, 14 and 28 days after in vitro incubation with differentiation media, using nano-liquid chromatography electrospray ionization tandem mass spectrometry analysis. This was part of a preliminary study of the behavior of differentiated adipose stem cells for use in a rabbit model of laryngoplasty. Data description The data comprise .MGF, .RAW, .MZID, and .XLSX, lists of peaks, peptides and proteins identified by nano-flow liquid chromatography electrospray ionization tandem mass spectrometry analysis upon incubation with non-differentiating (ND) or chondrogenic differentiating (CHD) media (ProteomeXchange ID PXD010236). XLSX files contain the following information: day 1 CT (control, N = 3499 proteins), day 14 ND (N = 3106 proteins), day 28 ND (N = 3116 proteins), day 14 CHD (N = 2901 proteins), and day 28 CHD (N = 2876 proteins). Proteins are characterized with respect to their − 10lgP value, percent coverage, number of total as well as unique peptides after trypsin digestion, derivatization method (carbamidomethylation, oxidation, or combined carbamidomethylation + oxidation), average mass, and include a full description.http://link.springer.com/article/10.1186/s13104-018-3750-6ProteomicsMass spectrometryAdipose mesenchymal stem cellsRegenerative medicineChondrogenic differentiation time-course |
| spellingShingle | Jeffrey R. Janus Stephen G. Voss Benjamin J. Madden Mary Cristine Charlesworth Michael S. Oldenburg Dale Ekbom Serban San-Marina Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype BMC Research Notes Proteomics Mass spectrometry Adipose mesenchymal stem cells Regenerative medicine Chondrogenic differentiation time-course |
| title | Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype |
| title_full | Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype |
| title_fullStr | Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype |
| title_full_unstemmed | Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype |
| title_short | Time-course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype |
| title_sort | time course mass spectrometry data of adipose mesenchymal stem cells acquiring chondrogenic phenotype |
| topic | Proteomics Mass spectrometry Adipose mesenchymal stem cells Regenerative medicine Chondrogenic differentiation time-course |
| url | http://link.springer.com/article/10.1186/s13104-018-3750-6 |
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