Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers
Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided int...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2019-08-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/1091 |
| _version_ | 1797394204104589312 |
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| author | Ilaria Marcotuli Andrea Mazzeo Domenica Nigro Stefania Lucia Giove Angelica Giancaspro Pasqualina Colasuonno Željko Prgomet Iva Prgomet Annalisa Tarantino Giuseppe Ferrara Agata Gadaleta |
| author_facet | Ilaria Marcotuli Andrea Mazzeo Domenica Nigro Stefania Lucia Giove Angelica Giancaspro Pasqualina Colasuonno Željko Prgomet Iva Prgomet Annalisa Tarantino Giuseppe Ferrara Agata Gadaleta |
| author_sort | Ilaria Marcotuli |
| collection | DOAJ |
| description |
Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided into two subgroups: uniferous (only main crop) and biferous (breba and main crop), was investigated using 49 microsatellite markers.
A total of 70 alleles were generated, of which 64 (91.4%) showed a polymorphic pattern indicating high level of genetic diversity within the studied collection. The mean heterozygosity over the 64 single locus microsatellites was 0.33 and the expected and observed averaged variance were 16.50 and 184.08, respectively.
The 139 fig genotypes formed two clusters in the PCoA analysis, suggesting a division between Italian and Croatian genotypes. Moreover, the fig accessions could be divided into two main clusters based on the STRUCTURE analysis according to the biological type, uniferous or biferous, with partly overlapping varieties. In conclusion, our results demonstrated that molecular markers were able to discriminate among genotypes and useful for the authentication of fig tree varieties (homonymies and synonymies).
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| first_indexed | 2024-03-09T00:16:06Z |
| format | Article |
| id | doaj.art-6401163894ac41cabdb964dd2547db25 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:16:06Z |
| publishDate | 2019-08-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-6401163894ac41cabdb964dd2547db252023-12-12T07:54:13ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052019-08-0118410.24326/asphc.2019.4.9Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markersIlaria Marcotuli0Andrea Mazzeo1Domenica Nigro2Stefania Lucia Giove3Angelica Giancaspro4Pasqualina Colasuonno5Željko Prgomet6Iva Prgomet7Annalisa Tarantino8Giuseppe Ferrara9Agata Gadaleta10Department of Environmental and Territorial Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, ItalyDepartment of Environmental and Territorial Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, ItalyDepartment of Environmental and Territorial Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, ItalyDepartment of Environmental and Territorial Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, ItalyCollegium Fluminense Polytechnic of Rijeka, Trpimirova 2/V, 51000 – Rijeka, CroatiaSkink, Valalta bb, 52210 – Rovinj, CroatiaDepartment of Science Agriculture, Food and Environment, University of Foggia, via Napoli 25, 71122 – Foggia, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, ItalyDepartment of Environmental and Territorial Sciences, University of Bari “Aldo Moro”, via G. Amendola 165/A, 70126 – Bari, Italy Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided into two subgroups: uniferous (only main crop) and biferous (breba and main crop), was investigated using 49 microsatellite markers. A total of 70 alleles were generated, of which 64 (91.4%) showed a polymorphic pattern indicating high level of genetic diversity within the studied collection. The mean heterozygosity over the 64 single locus microsatellites was 0.33 and the expected and observed averaged variance were 16.50 and 184.08, respectively. The 139 fig genotypes formed two clusters in the PCoA analysis, suggesting a division between Italian and Croatian genotypes. Moreover, the fig accessions could be divided into two main clusters based on the STRUCTURE analysis according to the biological type, uniferous or biferous, with partly overlapping varieties. In conclusion, our results demonstrated that molecular markers were able to discriminate among genotypes and useful for the authentication of fig tree varieties (homonymies and synonymies). https://czasopisma.up.lublin.pl/index.php/asphc/article/view/1091figbrebaSSRsgenetic diversitypopulation structure |
| spellingShingle | Ilaria Marcotuli Andrea Mazzeo Domenica Nigro Stefania Lucia Giove Angelica Giancaspro Pasqualina Colasuonno Željko Prgomet Iva Prgomet Annalisa Tarantino Giuseppe Ferrara Agata Gadaleta Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers Acta Scientiarum Polonorum: Hortorum Cultus fig breba SSRs genetic diversity population structure |
| title | Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers |
| title_full | Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers |
| title_fullStr | Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers |
| title_full_unstemmed | Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers |
| title_short | Analysis of genetic diversity of Ficus carica L. (Moraceae) collection using simple sequence repeat (SSR) markers |
| title_sort | analysis of genetic diversity of ficus carica l moraceae collection using simple sequence repeat ssr markers |
| topic | fig breba SSRs genetic diversity population structure |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/1091 |
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