Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein Adsorption
The abnormal increase in low-density lipoprotein (LDL) in human blood is a main independent risk factor for the pathogenesis of atherosclerosis, whereas a reduced LDL level effectively lowers morbidity. It is important to develop LDL adsorption materials with high efficiency and selectivity, as well...
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2023-06-01
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| author | Fei Fang Hai-Yang Zhao Rui Wang Qi Chen Qiong-Yan Wang Qing-Hua Zhang |
| author_facet | Fei Fang Hai-Yang Zhao Rui Wang Qi Chen Qiong-Yan Wang Qing-Hua Zhang |
| author_sort | Fei Fang |
| collection | DOAJ |
| description | The abnormal increase in low-density lipoprotein (LDL) in human blood is a main independent risk factor for the pathogenesis of atherosclerosis, whereas a reduced LDL level effectively lowers morbidity. It is important to develop LDL adsorption materials with high efficiency and selectivity, as well as to simplify their fabrication processes. In this paper, polysulfone (PSF), sulfonated polysulfone (SPSF), and sulfonated polysulfone/dextran (SPSF/GLU) membranes were successfully fabricated for LDL adsorption using a solution casting technique. Attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy measurements confirmed the success of the preparation. The water contact angle decreased from 89.7 ± 3.4° (PSF) to 76.4 ± 3.2° (SPSF) and to 71.2 ± 1.9° (SPSF/GLU), respectively. BSA adsorption testing showed that the SPSF/GLU with surface enrichment of sulfonate groups and glycosyl groups possessed higher resistance to protein solution. The adsorption and desorption behaviors of the studied samples in single-protein or binary-protein solutions were systematically investigated by enzyme-linked immunosorbent assay (ELISA), The results showed that SPSF/GLU, which had excellent resistance to protein adsorption, possessed a similar adsorption capacity to that of PSF. SPSF membrane exhibited excellent selective affinity for LDL in single and binary protein solutions, suggesting potential applications in LDL removal. |
| first_indexed | 2024-03-11T01:36:09Z |
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| id | doaj.art-6401acffe38e4152ac693213f0606910 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T01:36:09Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-6401acffe38e4152ac693213f06069102023-11-18T16:57:43ZengMDPI AGMaterials1996-19442023-06-011613464110.3390/ma16134641Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein AdsorptionFei Fang0Hai-Yang Zhao1Rui Wang2Qi Chen3Qiong-Yan Wang4Qing-Hua Zhang5College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaResearch and Development Center, Zhejiang Sucon Silicone Co., Ltd., Shaoxing 312088, ChinaResearch and Development Center, Zhejiang Sucon Silicone Co., Ltd., Shaoxing 312088, ChinaResearch and Development Center, Zhejiang Sucon Silicone Co., Ltd., Shaoxing 312088, ChinaResearch and Development Center, Zhejiang Sucon Silicone Co., Ltd., Shaoxing 312088, ChinaCollege of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaThe abnormal increase in low-density lipoprotein (LDL) in human blood is a main independent risk factor for the pathogenesis of atherosclerosis, whereas a reduced LDL level effectively lowers morbidity. It is important to develop LDL adsorption materials with high efficiency and selectivity, as well as to simplify their fabrication processes. In this paper, polysulfone (PSF), sulfonated polysulfone (SPSF), and sulfonated polysulfone/dextran (SPSF/GLU) membranes were successfully fabricated for LDL adsorption using a solution casting technique. Attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy measurements confirmed the success of the preparation. The water contact angle decreased from 89.7 ± 3.4° (PSF) to 76.4 ± 3.2° (SPSF) and to 71.2 ± 1.9° (SPSF/GLU), respectively. BSA adsorption testing showed that the SPSF/GLU with surface enrichment of sulfonate groups and glycosyl groups possessed higher resistance to protein solution. The adsorption and desorption behaviors of the studied samples in single-protein or binary-protein solutions were systematically investigated by enzyme-linked immunosorbent assay (ELISA), The results showed that SPSF/GLU, which had excellent resistance to protein adsorption, possessed a similar adsorption capacity to that of PSF. SPSF membrane exhibited excellent selective affinity for LDL in single and binary protein solutions, suggesting potential applications in LDL removal.https://www.mdpi.com/1996-1944/16/13/4641atherosclerosispolysulfonelow-density lipoproteinenzyme-linked immunosorbent assay |
| spellingShingle | Fei Fang Hai-Yang Zhao Rui Wang Qi Chen Qiong-Yan Wang Qing-Hua Zhang Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein Adsorption Materials atherosclerosis polysulfone low-density lipoprotein enzyme-linked immunosorbent assay |
| title | Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein Adsorption |
| title_full | Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein Adsorption |
| title_fullStr | Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein Adsorption |
| title_full_unstemmed | Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein Adsorption |
| title_short | Fabrication and Study of Dextran/Sulfonated Polysulfone Blend Membranes for Low-Density Lipoprotein Adsorption |
| title_sort | fabrication and study of dextran sulfonated polysulfone blend membranes for low density lipoprotein adsorption |
| topic | atherosclerosis polysulfone low-density lipoprotein enzyme-linked immunosorbent assay |
| url | https://www.mdpi.com/1996-1944/16/13/4641 |
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