LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid Obesity
This study investigated the importance of a metabolomic analysis in a complex disease such as nonalcoholic steatohepatitis (NASH) associated with obesity. Using an untargeted metabolomics technique, we studied blood metabolites in 216 morbidly obese women with liver histological diagnosis. A total o...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/9789 |
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| author | Laia Bertran Jordi Capellades Sonia Abelló Joan Durán-Bertran Carmen Aguilar Salomé Martinez Fàtima Sabench Xavier Correig Oscar Yanes Teresa Auguet Cristóbal Richart |
| author_facet | Laia Bertran Jordi Capellades Sonia Abelló Joan Durán-Bertran Carmen Aguilar Salomé Martinez Fàtima Sabench Xavier Correig Oscar Yanes Teresa Auguet Cristóbal Richart |
| author_sort | Laia Bertran |
| collection | DOAJ |
| description | This study investigated the importance of a metabolomic analysis in a complex disease such as nonalcoholic steatohepatitis (NASH) associated with obesity. Using an untargeted metabolomics technique, we studied blood metabolites in 216 morbidly obese women with liver histological diagnosis. A total of 172 patients were diagnosed with nonalcoholic fatty liver disease (NAFLD), and 44 were diagnosed with normal liver (NL). Patients with NAFLD were classified into simple steatosis (<i>n</i> = 66) and NASH (<i>n</i> = 106) categories. A comparative analysis of metabolites levels between NASH and NL demonstrated significant differences in lipid metabolites and derivatives, mainly from the phospholipid group. In NASH, there were increased levels of several phosphatidylinositols and phosphatidylethanolamines, as well as isolated metabolites such as diacylglycerol 34:1, lyso-phosphatidylethanolamine 20:3 and sphingomyelin 38:1. By contrast, there were decreased levels of acylcarnitines, sphingomyelins and linoleic acid. These findings may facilitate identification studies of the main pathogenic metabolic pathways related to NASH and may also have a possible applicability in a panel of metabolites to be used as biomarkers in future algorithms of the disease diagnosis and its follow-up. Further confirmatory studies in groups with different ages and sexes are necessary. |
| first_indexed | 2024-03-11T02:22:29Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:22:29Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-64026be826af4fa58f9e93ab82c645fa2023-11-18T10:44:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-012412978910.3390/ijms24129789LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid ObesityLaia Bertran0Jordi Capellades1Sonia Abelló2Joan Durán-Bertran3Carmen Aguilar4Salomé Martinez5Fàtima Sabench6Xavier Correig7Oscar Yanes8Teresa Auguet9Cristóbal Richart10Grup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada (URV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43005 Tarragona, SpainDepartment of Electronic Engineering, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43007 Tarragona, SpainServei de Recursos Científics i Tècnics, Universitat Rovira i Virgili, 43007 Tarragona, SpainGrup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada (URV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43005 Tarragona, SpainGrup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada (URV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43005 Tarragona, SpainGrup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada (URV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43005 Tarragona, SpainGrup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada (URV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43005 Tarragona, SpainDepartment of Electronic Engineering, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43007 Tarragona, SpainDepartment of Electronic Engineering, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43007 Tarragona, SpainGrup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada (URV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43005 Tarragona, SpainGrup de Recerca GEMMAIR (AGAUR)-Medicina Aplicada (URV), Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, Institut d’Investigació Sanitària Pere Virgili, 43005 Tarragona, SpainThis study investigated the importance of a metabolomic analysis in a complex disease such as nonalcoholic steatohepatitis (NASH) associated with obesity. Using an untargeted metabolomics technique, we studied blood metabolites in 216 morbidly obese women with liver histological diagnosis. A total of 172 patients were diagnosed with nonalcoholic fatty liver disease (NAFLD), and 44 were diagnosed with normal liver (NL). Patients with NAFLD were classified into simple steatosis (<i>n</i> = 66) and NASH (<i>n</i> = 106) categories. A comparative analysis of metabolites levels between NASH and NL demonstrated significant differences in lipid metabolites and derivatives, mainly from the phospholipid group. In NASH, there were increased levels of several phosphatidylinositols and phosphatidylethanolamines, as well as isolated metabolites such as diacylglycerol 34:1, lyso-phosphatidylethanolamine 20:3 and sphingomyelin 38:1. By contrast, there were decreased levels of acylcarnitines, sphingomyelins and linoleic acid. These findings may facilitate identification studies of the main pathogenic metabolic pathways related to NASH and may also have a possible applicability in a panel of metabolites to be used as biomarkers in future algorithms of the disease diagnosis and its follow-up. Further confirmatory studies in groups with different ages and sexes are necessary.https://www.mdpi.com/1422-0067/24/12/9789nonalcoholic fatty liver diseasenonalcoholic steatohepatitismorbid obesitymetabolomicslipidomics |
| spellingShingle | Laia Bertran Jordi Capellades Sonia Abelló Joan Durán-Bertran Carmen Aguilar Salomé Martinez Fàtima Sabench Xavier Correig Oscar Yanes Teresa Auguet Cristóbal Richart LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid Obesity International Journal of Molecular Sciences nonalcoholic fatty liver disease nonalcoholic steatohepatitis morbid obesity metabolomics lipidomics |
| title | LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid Obesity |
| title_full | LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid Obesity |
| title_fullStr | LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid Obesity |
| title_full_unstemmed | LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid Obesity |
| title_short | LC/MS-Based Untargeted Metabolomics Study in Women with Nonalcoholic Steatohepatitis Associated with Morbid Obesity |
| title_sort | lc ms based untargeted metabolomics study in women with nonalcoholic steatohepatitis associated with morbid obesity |
| topic | nonalcoholic fatty liver disease nonalcoholic steatohepatitis morbid obesity metabolomics lipidomics |
| url | https://www.mdpi.com/1422-0067/24/12/9789 |
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