Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt.
B. fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00498/full |
| _version_ | 1817986159707422720 |
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| author | Waheed eAkram Tehmina eAnjum Basharat eAli |
| author_facet | Waheed eAkram Tehmina eAnjum Basharat eAli |
| author_sort | Waheed eAkram |
| collection | DOAJ |
| description | B. fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were again treated with PAA and fungal pathogen in either combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contribute to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses. |
| first_indexed | 2024-04-14T00:06:42Z |
| format | Article |
| id | doaj.art-6403f6dd6a774101881b46c61883406c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T00:06:42Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-6403f6dd6a774101881b46c61883406c2022-12-22T02:23:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-04-01710.3389/fpls.2016.00498184268Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt.Waheed eAkram0Tehmina eAnjum1Basharat eAli2Department of Microbiology and Molecular Genetics. University of the Punjab. Department of Microbiology and Molecular Genetics. University of the Punjab. Department of Microbiology and Molecular Genetics. University of the Punjab. B. fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were again treated with PAA and fungal pathogen in either combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contribute to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00498/fullBacillusTomatophenylacetic acidinduced systemic resistance (ISR)Fusarium Wilt |
| spellingShingle | Waheed eAkram Tehmina eAnjum Basharat eAli Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt. Frontiers in Plant Science Bacillus Tomato phenylacetic acid induced systemic resistance (ISR) Fusarium Wilt |
| title | Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt. |
| title_full | Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt. |
| title_fullStr | Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt. |
| title_full_unstemmed | Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt. |
| title_short | Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against fusarium wilt. |
| title_sort | phenylacetic acid is isr determinant produced by bacillus fortis iags162 which involves extensive re modulation in metabolomics of tomato to protect against fusarium wilt |
| topic | Bacillus Tomato phenylacetic acid induced systemic resistance (ISR) Fusarium Wilt |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00498/full |
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