Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms
Objectives: The goal of this study was to isolate, molecularly identify, and antibiogram of Staphylococcus aureus, as well as to investigate risk factors for subclinical mastitis in the Jhenaidah and Chuadanga districts of Bangladesh. Materials and Methods: A total of 100 milk samples were collecte...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2021-02-01
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| Series: | Veterinary Research Notes |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=116426 |
| _version_ | 1811189907558760448 |
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| author | Md. Abu Zihad Md. Ahosanul Haque Shahid Md. Muket Mahmud Ajran Kabir Md. Shahriar Kamal Jannatun Naim Md. Liakot Hossen KHM Nazmul Hussain Nazir |
| author_facet | Md. Abu Zihad Md. Ahosanul Haque Shahid Md. Muket Mahmud Ajran Kabir Md. Shahriar Kamal Jannatun Naim Md. Liakot Hossen KHM Nazmul Hussain Nazir |
| author_sort | Md. Abu Zihad |
| collection | DOAJ |
| description | Objectives: The goal of this study was to isolate, molecularly identify, and antibiogram of Staphylococcus aureus, as well as to investigate risk factors for subclinical mastitis in the Jhenaidah and Chuadanga districts of Bangladesh.
Materials and Methods: A total of 100 milk samples were collected from apparently healthy goats at various farms in the Jhenaidah and Chuadanga districts. To detect samples with subclinical mastitis (SCM), the California Mastitis Test (CMT) was done. S. aureus was isolated and identified from milk samples that tested positive for CMT using a combination of standard bacteriological examinations, biochemical tests, and PCR confirmation. The disc diffusion test was used to determine the antibiogram status of the isolates. The risk factors for SCM in goats were analyzed using a semi-structured questionnaire with thirteen variables.
Results: Some characteristics were significantly associated with goat mastitis, including farm, breed, shed construction, floor surface condition, and farm adviser. The CMT identified 52 positive samples for SCM, with 73.07% (n = 38/52) suggesting the presence of the nuc gene. Antibiotic susceptibility testing revealed that the isolated S. aureus was totally resistant to the antibiotics Penicillin, Oxacillin, and Vancomycin (21%). On the other hand, Tetracycline, Gentamycin, Norfloxacin, and Levofloxacin were all susceptible to all isolates.
Conclusion: SCM is a severe problem in goats in Jhenaidah and Chuadanga districts. To minimize the risk of SCM infection in goats, hygiene precautions can be followed, and education among goat farm owners and farmers can be improved. [Vet. Res. Notes 2021; 1(2.000): 17-22] |
| first_indexed | 2024-04-11T14:41:41Z |
| format | Article |
| id | doaj.art-6408340e531c483c9503b14a943b8d7e |
| institution | Directory Open Access Journal |
| issn | 2790-2218 |
| language | English |
| last_indexed | 2024-04-11T14:41:41Z |
| publishDate | 2021-02-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Veterinary Research Notes |
| spelling | doaj.art-6408340e531c483c9503b14a943b8d7e2022-12-22T04:17:50ZengNetwork for the Veterinarians of BangladeshVeterinary Research Notes2790-22182021-02-0112172210.5455/vrn.2021.a4116426Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farmsMd. Abu Zihad0Md. Ahosanul Haque Shahid1Md. Muket Mahmud2Ajran Kabir3Md. Shahriar Kamal4Jannatun Naim5Md. Liakot Hossen6KHM Nazmul Hussain Nazir7Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. & Department of Microbiology, School of Biological Science, Primeasia University, Dhaka 1213, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh Livestock Officer, Proyas Manobik Unnayan Society, Chapainawabganj 6300, Bangladesh Department of Microbiology, Jhenaidah Government Veterinary College, Jhenaidah 7300, Bangladesh Department of Physiology, Biochemistry and Pharmacology, Jhenaidah Government Veterinary College, Jhenaidah 7300, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.Objectives: The goal of this study was to isolate, molecularly identify, and antibiogram of Staphylococcus aureus, as well as to investigate risk factors for subclinical mastitis in the Jhenaidah and Chuadanga districts of Bangladesh. Materials and Methods: A total of 100 milk samples were collected from apparently healthy goats at various farms in the Jhenaidah and Chuadanga districts. To detect samples with subclinical mastitis (SCM), the California Mastitis Test (CMT) was done. S. aureus was isolated and identified from milk samples that tested positive for CMT using a combination of standard bacteriological examinations, biochemical tests, and PCR confirmation. The disc diffusion test was used to determine the antibiogram status of the isolates. The risk factors for SCM in goats were analyzed using a semi-structured questionnaire with thirteen variables. Results: Some characteristics were significantly associated with goat mastitis, including farm, breed, shed construction, floor surface condition, and farm adviser. The CMT identified 52 positive samples for SCM, with 73.07% (n = 38/52) suggesting the presence of the nuc gene. Antibiotic susceptibility testing revealed that the isolated S. aureus was totally resistant to the antibiotics Penicillin, Oxacillin, and Vancomycin (21%). On the other hand, Tetracycline, Gentamycin, Norfloxacin, and Levofloxacin were all susceptible to all isolates. Conclusion: SCM is a severe problem in goats in Jhenaidah and Chuadanga districts. To minimize the risk of SCM infection in goats, hygiene precautions can be followed, and education among goat farm owners and farmers can be improved. [Vet. Res. Notes 2021; 1(2.000): 17-22]http://www.ejmanager.com/fulltextpdf.php?mno=116426antibiogram; nuc gene; pcr; subclinical mastitis; s. aureus |
| spellingShingle | Md. Abu Zihad Md. Ahosanul Haque Shahid Md. Muket Mahmud Ajran Kabir Md. Shahriar Kamal Jannatun Naim Md. Liakot Hossen KHM Nazmul Hussain Nazir Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms Veterinary Research Notes antibiogram; nuc gene; pcr; subclinical mastitis; s. aureus |
| title | Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms |
| title_full | Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms |
| title_fullStr | Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms |
| title_full_unstemmed | Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms |
| title_short | Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms |
| title_sort | molecular detection antibiogram and risk factor analysis of staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms |
| topic | antibiogram; nuc gene; pcr; subclinical mastitis; s. aureus |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=116426 |
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