Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent
This paper reports the MEPS-HPLC-DAD method for the simultaneous determination of 12 azole drugs (bifonazole, butoconazole, clotrimazole, econazole, itraconazole, ketoconazole, miconazole, posaconazole, ravuconazole, terconazole, tioconazole and voriconazole) administered to treat different systemic...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/14756366.2017.1354858 |
| _version_ | 1818216456985247744 |
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| author | Cristina Campestre Marcello Locatelli Paolo Guglielmi Elisa De Luca Giuseppe Bellagamba Sergio Menta Gokhan Zengin Christian Celia Luisa Di Marzio Simone Carradori |
| author_facet | Cristina Campestre Marcello Locatelli Paolo Guglielmi Elisa De Luca Giuseppe Bellagamba Sergio Menta Gokhan Zengin Christian Celia Luisa Di Marzio Simone Carradori |
| author_sort | Cristina Campestre |
| collection | DOAJ |
| description | This paper reports the MEPS-HPLC-DAD method for the simultaneous determination of 12 azole drugs (bifonazole, butoconazole, clotrimazole, econazole, itraconazole, ketoconazole, miconazole, posaconazole, ravuconazole, terconazole, tioconazole and voriconazole) administered to treat different systemic and topical fungal infections, in biological samples. Azole drugs separation was performed in 36 min. The analytical method was validated in the ranges as follows: 0.02–5 μg mL−1 for ravuconazole; 0.2–5 μg mL−1 for terconazole; 0.05–5 μg mL−1 for the other compounds. Human plasma and urine were used as biological samples during the analysis, while benzyl-4-hydroxybenzoate was used as an internal standard. The precision (RSD%) and trueness (Bias%) values fulfill with International Guidelines requirements. To the best of our knowledge, this is the first HPLC-DAD procedure coupled to MEPS, which provides the simultaneous analysis of 12 azole drugs, available in the market, in human plasma and urine. Moreover, the method was successfully applied for the quantitative determination of two model drugs (itraconazole and miconazole) after oral administration in real samples. |
| first_indexed | 2024-12-12T06:52:16Z |
| format | Article |
| id | doaj.art-640a894023644d0db4451d43710ddf8a |
| institution | Directory Open Access Journal |
| issn | 1475-6366 1475-6374 |
| language | English |
| last_indexed | 2024-12-12T06:52:16Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj.art-640a894023644d0db4451d43710ddf8a2022-12-22T00:34:03ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742017-01-013211053106310.1080/14756366.2017.13548581354858Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbentCristina Campestre0Marcello Locatelli1Paolo Guglielmi2Elisa De Luca3Giuseppe Bellagamba4Sergio Menta5Gokhan Zengin6Christian Celia7Luisa Di Marzio8Simone Carradori9University of Chieti – Pescara “G. d’Annunzio”University of Chieti – Pescara “G. d’Annunzio”Sapienza University of RomeUniversity of Chieti – Pescara “G. d’Annunzio”University of Chieti – Pescara “G. d’Annunzio”Sapienza University of RomeSelcuk UniversityUniversity of Chieti – Pescara “G. d’Annunzio”University of Chieti – Pescara “G. d’Annunzio”University of Chieti – Pescara “G. d’Annunzio”This paper reports the MEPS-HPLC-DAD method for the simultaneous determination of 12 azole drugs (bifonazole, butoconazole, clotrimazole, econazole, itraconazole, ketoconazole, miconazole, posaconazole, ravuconazole, terconazole, tioconazole and voriconazole) administered to treat different systemic and topical fungal infections, in biological samples. Azole drugs separation was performed in 36 min. The analytical method was validated in the ranges as follows: 0.02–5 μg mL−1 for ravuconazole; 0.2–5 μg mL−1 for terconazole; 0.05–5 μg mL−1 for the other compounds. Human plasma and urine were used as biological samples during the analysis, while benzyl-4-hydroxybenzoate was used as an internal standard. The precision (RSD%) and trueness (Bias%) values fulfill with International Guidelines requirements. To the best of our knowledge, this is the first HPLC-DAD procedure coupled to MEPS, which provides the simultaneous analysis of 12 azole drugs, available in the market, in human plasma and urine. Moreover, the method was successfully applied for the quantitative determination of two model drugs (itraconazole and miconazole) after oral administration in real samples.http://dx.doi.org/10.1080/14756366.2017.1354858MEPS-HPLC-DADmethod developmentplasma and urinesample preparationazole antifungal drugs |
| spellingShingle | Cristina Campestre Marcello Locatelli Paolo Guglielmi Elisa De Luca Giuseppe Bellagamba Sergio Menta Gokhan Zengin Christian Celia Luisa Di Marzio Simone Carradori Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent Journal of Enzyme Inhibition and Medicinal Chemistry MEPS-HPLC-DAD method development plasma and urine sample preparation azole antifungal drugs |
| title | Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent |
| title_full | Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent |
| title_fullStr | Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent |
| title_full_unstemmed | Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent |
| title_short | Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent |
| title_sort | analysis of imidazoles and triazoles in biological samples after microextraction by packed sorbent |
| topic | MEPS-HPLC-DAD method development plasma and urine sample preparation azole antifungal drugs |
| url | http://dx.doi.org/10.1080/14756366.2017.1354858 |
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