Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle
ABSTRACT: Propolis is a bee product, which has long been used in folk medicine for the management of different diseases. In this study we evaluated the analgesic and anti-inflammatory effects of a standard ethanolic extract of Bulgarian propolis (Et-Blg) in mice and its in vitro effect on airway smo...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2003-01-01
|
| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319325800 |
| _version_ | 1818311604502003712 |
|---|---|
| author | Niraldo Paulino Andreia Pires Dantas Vassya Bankova Daniela Taggliari Longhi Amarilis Scremin Solange Lisboa de Castro João Batista Calixto |
| author_facet | Niraldo Paulino Andreia Pires Dantas Vassya Bankova Daniela Taggliari Longhi Amarilis Scremin Solange Lisboa de Castro João Batista Calixto |
| author_sort | Niraldo Paulino |
| collection | DOAJ |
| description | ABSTRACT: Propolis is a bee product, which has long been used in folk medicine for the management of different diseases. In this study we evaluated the analgesic and anti-inflammatory effects of a standard ethanolic extract of Bulgarian propolis (Et-Blg) in mice and its in vitro effect on airway smooth muscle. Et-Blg inhibited acetic acid-induced abdominal contortions with an ID50 = 7.4 ± 0.7 mg · kg-1. In the formalin test, the extract caused a significant reduction in pain in mice treated with 100 mg · kg-1 Et-Blg during the neurogenic phase and for the inflammatory phase with all doses of the extract, with an ID50 = 2.5 ± 0.4 mg · kg-1. Et-Blg inhibited also the capsaicin-induced ear edema in mice; however, this extract was ineffective when assessed in the tail-flick and hot-plate thermal assays. The analgesic effect of Et-Blg was associated with the inhibition of inflammatory responses and not to a simple irritation of nervous terminals. In vitro, this extract inhibited the contraction of trachea smooth muscle induced by histamine (IC50 = 50 ± 5 μg · mL-1), capsaicin (IC50 = 26.8 ± 3 μg · mL-1), 80 mM KCl (IC50 = 27.8 ± 3 μg · mL-1), and carbachol (IC50 = 54 ± 2 μg · mL-1). |
| first_indexed | 2024-12-13T08:04:36Z |
| format | Article |
| id | doaj.art-640b580be0e24394bd4b8e2b06ad1df2 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-13T08:04:36Z |
| publishDate | 2003-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-640b580be0e24394bd4b8e2b06ad1df22022-12-21T23:54:21ZengElsevierJournal of Pharmacological Sciences1347-86132003-01-01933307313Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth MuscleNiraldo Paulino0Andreia Pires Dantas1Vassya Bankova2Daniela Taggliari Longhi3Amarilis Scremin4Solange Lisboa de Castro5João Batista Calixto6Grupo de Pesquisa e Desenvolvimento de Biofármacos (BIOFAR), Universidade do Sul de Santa Catarina, Tubarão, 88704-900, SC, BrazilDepartamento de Ultra-estrutura e Biologia Celular, Fundação Oswaldo Cruz, Rio de Janeiro, 21045-900, RJ, BrazilInstitute of Organic Chemistry with Centre of Phytochemistry, Sofia, BulgariaGrupo de Pesquisa e Desenvolvimento de Biofármacos (BIOFAR), Universidade do Sul de Santa Catarina, Tubarão, 88704-900, SC, BrazilGrupo de Pesquisa e Desenvolvimento de Biofármacos (BIOFAR), Universidade do Sul de Santa Catarina, Tubarão, 88704-900, SC, BrazilDepartamento de Ultra-estrutura e Biologia Celular, Fundação Oswaldo Cruz, Rio de Janeiro, 21045-900, RJ, BrazilDepartamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, 88015-420, SC, BrazilABSTRACT: Propolis is a bee product, which has long been used in folk medicine for the management of different diseases. In this study we evaluated the analgesic and anti-inflammatory effects of a standard ethanolic extract of Bulgarian propolis (Et-Blg) in mice and its in vitro effect on airway smooth muscle. Et-Blg inhibited acetic acid-induced abdominal contortions with an ID50 = 7.4 ± 0.7 mg · kg-1. In the formalin test, the extract caused a significant reduction in pain in mice treated with 100 mg · kg-1 Et-Blg during the neurogenic phase and for the inflammatory phase with all doses of the extract, with an ID50 = 2.5 ± 0.4 mg · kg-1. Et-Blg inhibited also the capsaicin-induced ear edema in mice; however, this extract was ineffective when assessed in the tail-flick and hot-plate thermal assays. The analgesic effect of Et-Blg was associated with the inhibition of inflammatory responses and not to a simple irritation of nervous terminals. In vitro, this extract inhibited the contraction of trachea smooth muscle induced by histamine (IC50 = 50 ± 5 μg · mL-1), capsaicin (IC50 = 26.8 ± 3 μg · mL-1), 80 mM KCl (IC50 = 27.8 ± 3 μg · mL-1), and carbachol (IC50 = 54 ± 2 μg · mL-1).http://www.sciencedirect.com/science/article/pii/S1347861319325800 |
| spellingShingle | Niraldo Paulino Andreia Pires Dantas Vassya Bankova Daniela Taggliari Longhi Amarilis Scremin Solange Lisboa de Castro João Batista Calixto Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle Journal of Pharmacological Sciences |
| title | Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle |
| title_full | Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle |
| title_fullStr | Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle |
| title_full_unstemmed | Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle |
| title_short | Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle |
| title_sort | bulgarian propolis induces analgesic and anti inflammatory effects in mice and inhibits in vitro contraction of airway smooth muscle |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319325800 |
| work_keys_str_mv | AT niraldopaulino bulgarianpropolisinducesanalgesicandantiinflammatoryeffectsinmiceandinhibitsinvitrocontractionofairwaysmoothmuscle AT andreiapiresdantas bulgarianpropolisinducesanalgesicandantiinflammatoryeffectsinmiceandinhibitsinvitrocontractionofairwaysmoothmuscle AT vassyabankova bulgarianpropolisinducesanalgesicandantiinflammatoryeffectsinmiceandinhibitsinvitrocontractionofairwaysmoothmuscle AT danielataggliarilonghi bulgarianpropolisinducesanalgesicandantiinflammatoryeffectsinmiceandinhibitsinvitrocontractionofairwaysmoothmuscle AT amarilisscremin bulgarianpropolisinducesanalgesicandantiinflammatoryeffectsinmiceandinhibitsinvitrocontractionofairwaysmoothmuscle AT solangelisboadecastro bulgarianpropolisinducesanalgesicandantiinflammatoryeffectsinmiceandinhibitsinvitrocontractionofairwaysmoothmuscle AT joaobatistacalixto bulgarianpropolisinducesanalgesicandantiinflammatoryeffectsinmiceandinhibitsinvitrocontractionofairwaysmoothmuscle |