Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline
Because of its excellent electrochemical properties, extreme redox performance, and ability to mediate the electron transfer between the electrode surface and the reaction site, polyaniline (PANI) is one of the most ideal and well-known conductive polymers for biosensor design. This research develop...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Düzce University
2022-04-01
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| Series: | Düzce Üniversitesi Bilim ve Teknoloji Dergisi |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/1460254 |
| _version_ | 1797300923155873792 |
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| author | Sedat Sürdem Soner Öztürk Nımet Yıldırım Tirgil Zeycan Kalkan Mehmet Fatih Oktem |
| author_facet | Sedat Sürdem Soner Öztürk Nımet Yıldırım Tirgil Zeycan Kalkan Mehmet Fatih Oktem |
| author_sort | Sedat Sürdem |
| collection | DOAJ |
| description | Because of its excellent electrochemical properties, extreme redox performance, and ability to mediate the electron transfer between the electrode surface and the reaction site, polyaniline (PANI) is one of the most ideal and well-known conductive polymers for biosensor design. This research developed an electrochemical enzymatic biosensor system with PANI film-coated screen printed electrodes (SPE) using the one-step direct electropolymerization process. PANI electropolymerization was performed in different acidic solutions, and the effects on the electrodeposition of the potential range, potential scan rate, and cycle number are discussed depending on these acidic solutions. The surface morphologies of films prepared with different processes were characterized by usıng the SEM (scanning electron microscopy) technique. A sensitive and selective catechol biosensor was developed by immobilizing the tyrosinase (Tyr) enzyme into PANI film combined with glutaraldehyde as a cross-linking agent. After optimizing the biosensor performance conditions, the developed biosensor measured catechol in green tea samples. |
| first_indexed | 2024-03-07T23:15:03Z |
| format | Article |
| id | doaj.art-640b9b4f0d024c33b5ede05d37e7c2c8 |
| institution | Directory Open Access Journal |
| issn | 2148-2446 |
| language | English |
| last_indexed | 2024-03-07T23:15:03Z |
| publishDate | 2022-04-01 |
| publisher | Düzce University |
| record_format | Article |
| series | Düzce Üniversitesi Bilim ve Teknoloji Dergisi |
| spelling | doaj.art-640b9b4f0d024c33b5ede05d37e7c2c82024-02-21T14:07:24ZengDüzce UniversityDüzce Üniversitesi Bilim ve Teknoloji Dergisi2148-24462022-04-0110292993910.29130/dubited.84433197Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of PolyanilineSedat Sürdem0Soner Öztürk1Nımet Yıldırım Tirgil2Zeycan Kalkan3Mehmet Fatih Oktem4TENMAK - National Boron Research InstituteTENMAK - National Boron Research InstituteANKARA YILDIRIM BEYAZIT UNIVERSITYANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİANKARA YILDIRIM BEYAZIT ÜNİVERSİTESİBecause of its excellent electrochemical properties, extreme redox performance, and ability to mediate the electron transfer between the electrode surface and the reaction site, polyaniline (PANI) is one of the most ideal and well-known conductive polymers for biosensor design. This research developed an electrochemical enzymatic biosensor system with PANI film-coated screen printed electrodes (SPE) using the one-step direct electropolymerization process. PANI electropolymerization was performed in different acidic solutions, and the effects on the electrodeposition of the potential range, potential scan rate, and cycle number are discussed depending on these acidic solutions. The surface morphologies of films prepared with different processes were characterized by usıng the SEM (scanning electron microscopy) technique. A sensitive and selective catechol biosensor was developed by immobilizing the tyrosinase (Tyr) enzyme into PANI film combined with glutaraldehyde as a cross-linking agent. After optimizing the biosensor performance conditions, the developed biosensor measured catechol in green tea samples.https://dergipark.org.tr/tr/download/article-file/1460254conducting polymerspolyanilineenzymatic biosensorscatechol analysisi̇letken polimerlerpolianilineenzimatik biyosensörlerkatekol aanalizi |
| spellingShingle | Sedat Sürdem Soner Öztürk Nımet Yıldırım Tirgil Zeycan Kalkan Mehmet Fatih Oktem Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline Düzce Üniversitesi Bilim ve Teknoloji Dergisi conducting polymers polyaniline enzymatic biosensors catechol analysis i̇letken polimerler polianilin eenzimatik biyosensörler katekol aanalizi |
| title | Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline |
| title_full | Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline |
| title_fullStr | Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline |
| title_full_unstemmed | Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline |
| title_short | Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline |
| title_sort | electrochemical enzymatic biosensor development by using different electropolymerization conditions of polyaniline |
| topic | conducting polymers polyaniline enzymatic biosensors catechol analysis i̇letken polimerler polianilin eenzimatik biyosensörler katekol aanalizi |
| url | https://dergipark.org.tr/tr/download/article-file/1460254 |
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