Preparation and Application of Plasmid DNA Reference Material in the Detection of Cronobacter in Infant Formula

Preparation and Application of Plasmid DNA Reference Material in the Detection of Cronobacter in Infant Formula

The housekeeping genes atpD, gyrB, infB, fusA and glnS in Cronobacter sakazakii were selected as target sequences to construct plasmid standards for rapidly detecting C. sakazakii. The constructed recombinant plasmids was verified by colony polymerase chain reaction (PCR) and sequencing and was chec...

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Bibliographic Details
Main Author:	MA Yueran, WANG Hui, FAN Huixia, ZHAO Hongyang, LU Xing’an, GAO Xiaofeng, KANG Wenyi
Format:	Article
Language:	English
Published:	China Food Publishing Company 2023-06-01
Series:	Shipin Kexue
Subjects:	cronobacter; house-keeping genes; plasmid standards; rapid detection
Online Access:	https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-12-039.pdf

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