Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis
Sour oranges (<i>Citrus aurantium</i> L.) are well known in the processing and cosmetics industries for the aromatic properties of their essential oils. Intercultivar genetic and aromatic diversity is not well documented. The objective of this study was to evaluate the impact of morpholo...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/11/6/1084 |
| _version_ | 1797532394280976384 |
|---|---|
| author | Vincent Ferrer Gilles Costantino Mathieu Paoli Noémie Paymal Carole Quinton Patrick Ollitrault Félix Tomi François Luro |
| author_facet | Vincent Ferrer Gilles Costantino Mathieu Paoli Noémie Paymal Carole Quinton Patrick Ollitrault Félix Tomi François Luro |
| author_sort | Vincent Ferrer |
| collection | DOAJ |
| description | Sour oranges (<i>Citrus aurantium</i> L.) are well known in the processing and cosmetics industries for the aromatic properties of their essential oils. Intercultivar genetic and aromatic diversity is not well documented. The objective of this study was to evaluate the impact of morphological selection and genetic mechanisms of varietal diversification (mutation or hybridization) on the aromatic and odor variability of sour orange essential oils. Forty-five sour orange accessions from INRAE-CIRAD citrus Biological Resources Center (France) were assessed for ten simple sequence repeat (SSR) and 54 single nucleotide polymorphism (SNP) markers, nine morphochemical fruit traits and with the aromatic components of leaf and peel essential oils. Thirty-nine sour oranges displayed no intercultivar molecular polymorphism and six genotypes originated from interspecific hybridizations involving sour orange, citron, pummelo or mandarin. The peel essential oil (PEO) diversity was low, in accordance with the genetic diversity. The predominance of limonene (>90%) prevents any possible correlation to be made between the composition and the variation in sensory profiles detected by panelists. Few compounds in the leaf essential oil (LEO), such as linalool, linalyl acetate, α-terpineol and geraniol were significantly different across sour oranges varieties. The morphological fruit attributes mainly used in varietal selection differed highly across the main genetically identical group of sour orange accessions. These results confirm that mutation can generate variability in aromatic compounds and aromas and that their exploitation requires an improvement in characterization processes. |
| first_indexed | 2024-03-10T10:59:27Z |
| format | Article |
| id | doaj.art-64133c4ec8814c1c8a78a5a1229023ef |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T10:59:27Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-64133c4ec8814c1c8a78a5a1229023ef2023-11-21T21:38:52ZengMDPI AGAgronomy2073-43952021-05-01116108410.3390/agronomy11061084Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial AnalysisVincent Ferrer0Gilles Costantino1Mathieu Paoli2Noémie Paymal3Carole Quinton4Patrick Ollitrault5Félix Tomi6François Luro7UMR AGAP Institut, CIRAD, INRAE, Institut Agro, University Montpellier, 20230 San Giuliano, FranceUMR AGAP Institut, CIRAD, INRAE, Institut Agro, University Montpellier, 20230 San Giuliano, FranceUMR SPE 6134, Equipe chimie et Biomasse, Université de Corse—CNRS, 20000 Ajaccio, FranceRémy Cointreau—Les Molières, 49124 Saint-Barthélemy-d’Anjou, FranceRémy Cointreau—Les Molières, 49124 Saint-Barthélemy-d’Anjou, FranceUMR AGAP Institut, CIRAD, INRAE, Institut Agro, University Montpellier, 20230 San Giuliano, FranceUMR SPE 6134, Equipe chimie et Biomasse, Université de Corse—CNRS, 20000 Ajaccio, FranceUMR AGAP Institut, CIRAD, INRAE, Institut Agro, University Montpellier, 20230 San Giuliano, FranceSour oranges (<i>Citrus aurantium</i> L.) are well known in the processing and cosmetics industries for the aromatic properties of their essential oils. Intercultivar genetic and aromatic diversity is not well documented. The objective of this study was to evaluate the impact of morphological selection and genetic mechanisms of varietal diversification (mutation or hybridization) on the aromatic and odor variability of sour orange essential oils. Forty-five sour orange accessions from INRAE-CIRAD citrus Biological Resources Center (France) were assessed for ten simple sequence repeat (SSR) and 54 single nucleotide polymorphism (SNP) markers, nine morphochemical fruit traits and with the aromatic components of leaf and peel essential oils. Thirty-nine sour oranges displayed no intercultivar molecular polymorphism and six genotypes originated from interspecific hybridizations involving sour orange, citron, pummelo or mandarin. The peel essential oil (PEO) diversity was low, in accordance with the genetic diversity. The predominance of limonene (>90%) prevents any possible correlation to be made between the composition and the variation in sensory profiles detected by panelists. Few compounds in the leaf essential oil (LEO), such as linalool, linalyl acetate, α-terpineol and geraniol were significantly different across sour oranges varieties. The morphological fruit attributes mainly used in varietal selection differed highly across the main genetically identical group of sour orange accessions. These results confirm that mutation can generate variability in aromatic compounds and aromas and that their exploitation requires an improvement in characterization processes.https://www.mdpi.com/2073-4395/11/6/1084peel and leaf essential oilsGC-MSSSRSNPPCAcitrus color index |
| spellingShingle | Vincent Ferrer Gilles Costantino Mathieu Paoli Noémie Paymal Carole Quinton Patrick Ollitrault Félix Tomi François Luro Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis Agronomy peel and leaf essential oils GC-MS SSR SNP PCA citrus color index |
| title | Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis |
| title_full | Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis |
| title_fullStr | Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis |
| title_full_unstemmed | Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis |
| title_short | Intercultivar Diversity of Sour Orange (<i>Citrus</i> <i>aurantium</i> L.) Based on Genetic Markers, Phenotypic Characteristics, Aromatic Compounds and Sensorial Analysis |
| title_sort | intercultivar diversity of sour orange i citrus i i aurantium i l based on genetic markers phenotypic characteristics aromatic compounds and sensorial analysis |
| topic | peel and leaf essential oils GC-MS SSR SNP PCA citrus color index |
| url | https://www.mdpi.com/2073-4395/11/6/1084 |
| work_keys_str_mv | AT vincentferrer intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis AT gillescostantino intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis AT mathieupaoli intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis AT noemiepaymal intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis AT carolequinton intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis AT patrickollitrault intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis AT felixtomi intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis AT francoisluro intercultivardiversityofsourorangeicitrusiiaurantiumilbasedongeneticmarkersphenotypiccharacteristicsaromaticcompoundsandsensorialanalysis |