overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheep
Saturated fatty acids (SFAs) can directly stimulate innate immune responses, thereby exacerbating inflammatory aspects of metabolic syndrome. Dietary SFAs act as ligands of Toll-like receptor 4 (TLR4), triggering associated signaling pathways. In this study, we investigated the role of TLR4 in palm...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2018-01-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/2058739218792976 |
| _version_ | 1818789572385964032 |
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| author | Xue Xu Mei-Yu Qi Shuang Liu Xu-Ting Song Jia-Nan Zhang Yu-Fei Zhai Ming-Hai Lu Hong-Bing Han Zheng-Xing Lian Yu-Chang Yao |
| author_facet | Xue Xu Mei-Yu Qi Shuang Liu Xu-Ting Song Jia-Nan Zhang Yu-Fei Zhai Ming-Hai Lu Hong-Bing Han Zheng-Xing Lian Yu-Chang Yao |
| author_sort | Xue Xu |
| collection | DOAJ |
| description | Saturated fatty acids (SFAs) can directly stimulate innate immune responses, thereby exacerbating inflammatory aspects of metabolic syndrome. Dietary SFAs act as ligands of Toll-like receptor 4 (TLR4), triggering associated signaling pathways. In this study, we investigated the role of TLR4 in palm oil SFA-associated inflammatory cytokine gene expression in monocytes/macrophages and adipose tissue using TLR4 -overexpressing genetically modified sheep. SFA stimulation resulted in upregulation of interleukin-6 ( IL-6 ), tumor necrosis factor-α ( TNF-α ), interleukin-8 ( IL-8 ), interferon-γ ( IFN-γ ), and interleukin-10 ( IL-10 ), and TLR4 overexpression enhanced such SFA-induced inflammatory cytokine expression. Moreover, SFAs markedly activated MyD88-dependent signaling, including IL-1 receptor–associated kinase 4 ( IRAK4 ), TNF receptor–associated factor 6 ( TRAF6 ), and nuclear factor-κB ( NF-κB ). Taken together, our results indicate that TLR4 overexpression enhances the SFA-induced inflammatory response through MyD88-dependent signaling in monocytes/macrophages and adipose tissue. |
| first_indexed | 2024-12-18T14:41:42Z |
| format | Article |
| id | doaj.art-64135cb0c88a4928a00ab8265b4becc7 |
| institution | Directory Open Access Journal |
| issn | 2058-7392 |
| language | English |
| last_indexed | 2024-12-18T14:41:42Z |
| publishDate | 2018-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-64135cb0c88a4928a00ab8265b4becc72022-12-21T21:04:23ZengSAGE PublishingEuropean Journal of Inflammation2058-73922018-01-011610.1177/2058739218792976overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheepXue Xu0Mei-Yu Qi1Shuang Liu2Xu-Ting Song3Jia-Nan Zhang4Yu-Fei Zhai5Ming-Hai Lu6Hong-Bing Han7Zheng-Xing Lian8Yu-Chang Yao9College of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaInstitute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaDepartment of Animal Science, Heilongjiang State Farms Science Technology Vocational College, Harbin, ChinaBeijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, ChinaBeijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, ChinaSaturated fatty acids (SFAs) can directly stimulate innate immune responses, thereby exacerbating inflammatory aspects of metabolic syndrome. Dietary SFAs act as ligands of Toll-like receptor 4 (TLR4), triggering associated signaling pathways. In this study, we investigated the role of TLR4 in palm oil SFA-associated inflammatory cytokine gene expression in monocytes/macrophages and adipose tissue using TLR4 -overexpressing genetically modified sheep. SFA stimulation resulted in upregulation of interleukin-6 ( IL-6 ), tumor necrosis factor-α ( TNF-α ), interleukin-8 ( IL-8 ), interferon-γ ( IFN-γ ), and interleukin-10 ( IL-10 ), and TLR4 overexpression enhanced such SFA-induced inflammatory cytokine expression. Moreover, SFAs markedly activated MyD88-dependent signaling, including IL-1 receptor–associated kinase 4 ( IRAK4 ), TNF receptor–associated factor 6 ( TRAF6 ), and nuclear factor-κB ( NF-κB ). Taken together, our results indicate that TLR4 overexpression enhances the SFA-induced inflammatory response through MyD88-dependent signaling in monocytes/macrophages and adipose tissue.https://doi.org/10.1177/2058739218792976 |
| spellingShingle | Xue Xu Mei-Yu Qi Shuang Liu Xu-Ting Song Jia-Nan Zhang Yu-Fei Zhai Ming-Hai Lu Hong-Bing Han Zheng-Xing Lian Yu-Chang Yao overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheep European Journal of Inflammation |
| title | overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheep |
| title_full | overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheep |
| title_fullStr | overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheep |
| title_full_unstemmed | overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheep |
| title_short | overexpression enhances saturated fatty acid–induced inflammatory cytokine gene expression in sheep |
| title_sort | overexpression enhances saturated fatty acid induced inflammatory cytokine gene expression in sheep |
| url | https://doi.org/10.1177/2058739218792976 |
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