Isolation and characterisation of oil palm LEAFY transcripts
LEAFY (LFY) is an important regulator of plant's reproductive system, regulating the transition from vegetative growth to flowering, and also acts as an upstream regulator of floral homeotic MADS-box genes in Arabidopsis. Owing to its importance in flower development, oil palm LFY transcripts w...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-07-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2018.1464949 |
| _version_ | 1811196514670739456 |
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| author | Sharmilah Vetaryan Yen-Yen Kwan Parameswari Namasivayam Chai-Ling Ho Sharifah Shahrul Rabiah Syed Alwee |
| author_facet | Sharmilah Vetaryan Yen-Yen Kwan Parameswari Namasivayam Chai-Ling Ho Sharifah Shahrul Rabiah Syed Alwee |
| author_sort | Sharmilah Vetaryan |
| collection | DOAJ |
| description | LEAFY (LFY) is an important regulator of plant's reproductive system, regulating the transition from vegetative growth to flowering, and also acts as an upstream regulator of floral homeotic MADS-box genes in Arabidopsis. Owing to its importance in flower development, oil palm LFY transcripts were isolated and the expression pattern was characterised to elucidate the role of LFY in oil palm. In addition, the gene expression pattern was analysed to determine a possible correlation of LFY to a type of floral abnormality in clonal palms termed mantling. In this study, three LFY transcripts were isolated from oil palm inflorescence tissue. The isolated cDNAs, OpLFY1, OpLFY2 and its splice variant, OpLFY2v were predicted to encode proteins each consisting of 430, 451 and 181 amino acids, respectively. OpLFY1 and OpLFY2 share 90% and 94% amino acid sequence identity to two different LFYs from Phoenix dactylifera, respectively. OpLFY1 was localised in all floral organ primordia. Quantitative polymerase chain reaction (qPCR) analysis revealed that OpLFY genes are expressed in shoot apical meristem and inflorescence tissues with the highest expression level. The expression of the OpLFY1 gene was significantly lower in stage 1 of inflorescence development in mantled palms compared with that of normal inflorescences, suggesting possible association of the gene with this somaclonal variation. |
| first_indexed | 2024-04-12T01:00:05Z |
| format | Article |
| id | doaj.art-64151817507c4ceeb1e29bd7acce9406 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-04-12T01:00:05Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-64151817507c4ceeb1e29bd7acce94062022-12-22T03:54:29ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302018-07-0132488889810.1080/13102818.2018.14649491464949Isolation and characterisation of oil palm LEAFY transcriptsSharmilah Vetaryan0Yen-Yen Kwan1Parameswari Namasivayam2Chai-Ling Ho3Sharifah Shahrul Rabiah Syed Alwee4Lengkuk TeknologiLengkuk TeknologiUniversiti Putra MalaysiaUniversiti Putra MalaysiaLengkuk TeknologiLEAFY (LFY) is an important regulator of plant's reproductive system, regulating the transition from vegetative growth to flowering, and also acts as an upstream regulator of floral homeotic MADS-box genes in Arabidopsis. Owing to its importance in flower development, oil palm LFY transcripts were isolated and the expression pattern was characterised to elucidate the role of LFY in oil palm. In addition, the gene expression pattern was analysed to determine a possible correlation of LFY to a type of floral abnormality in clonal palms termed mantling. In this study, three LFY transcripts were isolated from oil palm inflorescence tissue. The isolated cDNAs, OpLFY1, OpLFY2 and its splice variant, OpLFY2v were predicted to encode proteins each consisting of 430, 451 and 181 amino acids, respectively. OpLFY1 and OpLFY2 share 90% and 94% amino acid sequence identity to two different LFYs from Phoenix dactylifera, respectively. OpLFY1 was localised in all floral organ primordia. Quantitative polymerase chain reaction (qPCR) analysis revealed that OpLFY genes are expressed in shoot apical meristem and inflorescence tissues with the highest expression level. The expression of the OpLFY1 gene was significantly lower in stage 1 of inflorescence development in mantled palms compared with that of normal inflorescences, suggesting possible association of the gene with this somaclonal variation.http://dx.doi.org/10.1080/13102818.2018.1464949Elaeis guineensisgene expressionflower developmentsomaclonal variationmantlinginflorescence |
| spellingShingle | Sharmilah Vetaryan Yen-Yen Kwan Parameswari Namasivayam Chai-Ling Ho Sharifah Shahrul Rabiah Syed Alwee Isolation and characterisation of oil palm LEAFY transcripts Biotechnology & Biotechnological Equipment Elaeis guineensis gene expression flower development somaclonal variation mantling inflorescence |
| title | Isolation and characterisation of oil palm LEAFY transcripts |
| title_full | Isolation and characterisation of oil palm LEAFY transcripts |
| title_fullStr | Isolation and characterisation of oil palm LEAFY transcripts |
| title_full_unstemmed | Isolation and characterisation of oil palm LEAFY transcripts |
| title_short | Isolation and characterisation of oil palm LEAFY transcripts |
| title_sort | isolation and characterisation of oil palm leafy transcripts |
| topic | Elaeis guineensis gene expression flower development somaclonal variation mantling inflorescence |
| url | http://dx.doi.org/10.1080/13102818.2018.1464949 |
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