Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes
Insulin resistance (IR) plays a central role in the development of several metabolic diseases, which leads to increased morbidity and mortality rates, in addition to soaring health-care costs. Deep sea water (DSW) and fucoidans (FPS) have drawn much attention in recent years because of their potenti...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-02-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/16/2/48 |
| _version_ | 1811263948310183936 |
|---|---|
| author | Shan He Wei-Bing Peng Hong-Lei Zhou |
| author_facet | Shan He Wei-Bing Peng Hong-Lei Zhou |
| author_sort | Shan He |
| collection | DOAJ |
| description | Insulin resistance (IR) plays a central role in the development of several metabolic diseases, which leads to increased morbidity and mortality rates, in addition to soaring health-care costs. Deep sea water (DSW) and fucoidans (FPS) have drawn much attention in recent years because of their potential medical and pharmaceutical applications. This study investigated the effects and mechanisms of combination treatment of DSW and FPS in improving IR in HepG2 hepatocytes induced by a high glucose concentration. The results elucidated that co-treatment with DSW and FPS could synergistically repress hepatic glucose production and increase the glycogen level in IR-HepG2 cells. In addition, they stimulated the phosphorylation levels of the components of the insulin signaling pathway, including tyrosine phosphorylation of IRS-1, and serine phosphorylation of Akt and GSK-3β. Furthermore, they increased the phosphorylation of AMPK and ACC, which in turn decreased the intracellular triglyceride level. Taken together, these results suggested that co-treatment with DSW and FPS had a greater improving effect than DSW or FPS alone on IR. They might attenuate IR by targeting Akt/GSK-3β and AMPK pathways. These results may have some implications in the treatment of metabolic diseases. |
| first_indexed | 2024-04-12T19:55:37Z |
| format | Article |
| id | doaj.art-6415224d8c1949c1b68ab204f7a7547e |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-12T19:55:37Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6415224d8c1949c1b68ab204f7a7547e2022-12-22T03:18:41ZengMDPI AGMarine Drugs1660-33972018-02-011624810.3390/md16020048md16020048Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 HepatocytesShan He0Wei-Bing Peng1Hong-Lei Zhou2School of Pharmacology, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, ChinaSchool of Pharmacology, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaInsulin resistance (IR) plays a central role in the development of several metabolic diseases, which leads to increased morbidity and mortality rates, in addition to soaring health-care costs. Deep sea water (DSW) and fucoidans (FPS) have drawn much attention in recent years because of their potential medical and pharmaceutical applications. This study investigated the effects and mechanisms of combination treatment of DSW and FPS in improving IR in HepG2 hepatocytes induced by a high glucose concentration. The results elucidated that co-treatment with DSW and FPS could synergistically repress hepatic glucose production and increase the glycogen level in IR-HepG2 cells. In addition, they stimulated the phosphorylation levels of the components of the insulin signaling pathway, including tyrosine phosphorylation of IRS-1, and serine phosphorylation of Akt and GSK-3β. Furthermore, they increased the phosphorylation of AMPK and ACC, which in turn decreased the intracellular triglyceride level. Taken together, these results suggested that co-treatment with DSW and FPS had a greater improving effect than DSW or FPS alone on IR. They might attenuate IR by targeting Akt/GSK-3β and AMPK pathways. These results may have some implications in the treatment of metabolic diseases.http://www.mdpi.com/1660-3397/16/2/48deep-sea waterfucoidanIR-HepG2Akt/GSK-3β pathwayAMPK-ACC pathway |
| spellingShingle | Shan He Wei-Bing Peng Hong-Lei Zhou Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes Marine Drugs deep-sea water fucoidan IR-HepG2 Akt/GSK-3β pathway AMPK-ACC pathway |
| title | Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes |
| title_full | Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes |
| title_fullStr | Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes |
| title_full_unstemmed | Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes |
| title_short | Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes |
| title_sort | combination treatment of deep sea water and fucoidan attenuates high glucose induced insulin resistance in hepg2 hepatocytes |
| topic | deep-sea water fucoidan IR-HepG2 Akt/GSK-3β pathway AMPK-ACC pathway |
| url | http://www.mdpi.com/1660-3397/16/2/48 |
| work_keys_str_mv | AT shanhe combinationtreatmentofdeepseawaterandfucoidanattenuateshighglucoseinducedinsulinresistanceinhepg2hepatocytes AT weibingpeng combinationtreatmentofdeepseawaterandfucoidanattenuateshighglucoseinducedinsulinresistanceinhepg2hepatocytes AT hongleizhou combinationtreatmentofdeepseawaterandfucoidanattenuateshighglucoseinducedinsulinresistanceinhepg2hepatocytes |