Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation
Xiao Wu,1 Don Hayes Jr,2,3 Joseph B Zwischenberger,4 Robert J Kuhn,5 Heidi M Mansour1,61University of Kentucky, College of Pharmacy, Department of Pharmaceutical Sciences-Drug Development Division, Lexington, KY, USA; 2The Ohio State University College of Medicine, Departments of Pediatrics and Inte...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2013-02-01
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| Series: | Drug Design, Development and Therapy |
| Online Access: | http://www.dovepress.com/design-and-physicochemical-characterization-of-advanced-spray-dried-ta-a12115 |
| _version_ | 1818849759653265408 |
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| author | Wu X Hayes D Jr Zwischenberger JB Kuhn RJ Mansour HM |
| author_facet | Wu X Hayes D Jr Zwischenberger JB Kuhn RJ Mansour HM |
| author_sort | Wu X |
| collection | DOAJ |
| description | Xiao Wu,1 Don Hayes Jr,2,3 Joseph B Zwischenberger,4 Robert J Kuhn,5 Heidi M Mansour1,61University of Kentucky, College of Pharmacy, Department of Pharmaceutical Sciences-Drug Development Division, Lexington, KY, USA; 2The Ohio State University College of Medicine, Departments of Pediatrics and Internal Medicine, Lung and Heart-Lung Transplant Programs, Nationwide Children's Hospital, Columbus, OH, USA; 3The Ohio State University College of Medicine, The Davis Heart and Lung Research Institute, Columbus, OH, USA; 4University of Kentucky College of Medicine, Departments of Pediatrics, Biomedical Engineering, Diagnostic Radiology, and Surgery, Lexington, KY, USA; 5University of Kentucky, College of Pharmacy, Division of Pharmacy Practice and Science, Lexington, KY, USA; 6University of Kentucky, Center of Membrane Sciences, Lexington, KY, USAAbstract: The aim of this study was to design, develop, and optimize respirable tacrolimus microparticles and nanoparticles and multifunctional tacrolimus lung surfactant mimic particles for targeted dry powder inhalation delivery as a pulmonary nanomedicine. Particles were rationally designed and produced at different pump rates by advanced spray-drying particle engineering design from organic solution in closed mode. In addition, multifunctional tacrolimus lung surfactant mimic dry powder particles were prepared by co-dissolving tacrolimus and lung surfactant mimic phospholipids in methanol, followed by advanced co-spray-drying particle engineering design technology in closed mode. The lung surfactant mimic phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-[phosphor-rac-1-glycerol]. Laser diffraction particle sizing indicated that the particle size distributions were suitable for pulmonary delivery, whereas scanning electron microscopy imaging indicated that these particles had both optimal particle morphology and surface morphology. Increasing the pump rate percent of tacrolimus solution resulted in a larger particle size. X-ray powder diffraction patterns and differential scanning calorimetry thermograms indicated that spray drying produced particles with higher amounts of amorphous phase. X-ray powder diffraction and differential scanning calorimetry also confirmed the preservation of the phospholipid bilayer structure in the solid state for all engineered respirable particles. Furthermore, it was observed in hot-stage micrographs that raw tacrolimus displayed a liquid crystal transition following the main phase transition, which is consistent with its interfacial properties. Water vapor uptake and lyotropic phase transitions in the solid state at varying levels of relative humidity were determined by gravimetric vapor sorption technique. Water content in the various powders was very low and well within the levels necessary for dry powder inhalation, as quantified by Karl Fisher coulometric titration. Conclusively, advanced spray-drying particle engineering design from organic solution in closed mode was successfully used to design and optimize solid-state particles in the respirable size range necessary for targeted pulmonary delivery, particularly for the deep lung. These particles were dry, stable, and had optimal properties for dry powder inhalation as a novel pulmonary nanomedicine.Keywords: dry powder inhaler (DPI), pulmonary nanomedicine, lung transplant, immunosuppression, lung surfactant, phospholipid colloidal self-assemblies, solid-state particle engineering design, organic solution advanced spray drying |
| first_indexed | 2024-12-19T06:38:21Z |
| format | Article |
| id | doaj.art-6417c703fa9b4745aba9a9443cb17cb4 |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-19T06:38:21Z |
| publishDate | 2013-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-6417c703fa9b4745aba9a9443cb17cb42022-12-21T20:32:11ZengDove Medical PressDrug Design, Development and Therapy1177-88812013-02-012013default5972Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalationWu XHayes D JrZwischenberger JBKuhn RJMansour HMXiao Wu,1 Don Hayes Jr,2,3 Joseph B Zwischenberger,4 Robert J Kuhn,5 Heidi M Mansour1,61University of Kentucky, College of Pharmacy, Department of Pharmaceutical Sciences-Drug Development Division, Lexington, KY, USA; 2The Ohio State University College of Medicine, Departments of Pediatrics and Internal Medicine, Lung and Heart-Lung Transplant Programs, Nationwide Children's Hospital, Columbus, OH, USA; 3The Ohio State University College of Medicine, The Davis Heart and Lung Research Institute, Columbus, OH, USA; 4University of Kentucky College of Medicine, Departments of Pediatrics, Biomedical Engineering, Diagnostic Radiology, and Surgery, Lexington, KY, USA; 5University of Kentucky, College of Pharmacy, Division of Pharmacy Practice and Science, Lexington, KY, USA; 6University of Kentucky, Center of Membrane Sciences, Lexington, KY, USAAbstract: The aim of this study was to design, develop, and optimize respirable tacrolimus microparticles and nanoparticles and multifunctional tacrolimus lung surfactant mimic particles for targeted dry powder inhalation delivery as a pulmonary nanomedicine. Particles were rationally designed and produced at different pump rates by advanced spray-drying particle engineering design from organic solution in closed mode. In addition, multifunctional tacrolimus lung surfactant mimic dry powder particles were prepared by co-dissolving tacrolimus and lung surfactant mimic phospholipids in methanol, followed by advanced co-spray-drying particle engineering design technology in closed mode. The lung surfactant mimic phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-[phosphor-rac-1-glycerol]. Laser diffraction particle sizing indicated that the particle size distributions were suitable for pulmonary delivery, whereas scanning electron microscopy imaging indicated that these particles had both optimal particle morphology and surface morphology. Increasing the pump rate percent of tacrolimus solution resulted in a larger particle size. X-ray powder diffraction patterns and differential scanning calorimetry thermograms indicated that spray drying produced particles with higher amounts of amorphous phase. X-ray powder diffraction and differential scanning calorimetry also confirmed the preservation of the phospholipid bilayer structure in the solid state for all engineered respirable particles. Furthermore, it was observed in hot-stage micrographs that raw tacrolimus displayed a liquid crystal transition following the main phase transition, which is consistent with its interfacial properties. Water vapor uptake and lyotropic phase transitions in the solid state at varying levels of relative humidity were determined by gravimetric vapor sorption technique. Water content in the various powders was very low and well within the levels necessary for dry powder inhalation, as quantified by Karl Fisher coulometric titration. Conclusively, advanced spray-drying particle engineering design from organic solution in closed mode was successfully used to design and optimize solid-state particles in the respirable size range necessary for targeted pulmonary delivery, particularly for the deep lung. These particles were dry, stable, and had optimal properties for dry powder inhalation as a novel pulmonary nanomedicine.Keywords: dry powder inhaler (DPI), pulmonary nanomedicine, lung transplant, immunosuppression, lung surfactant, phospholipid colloidal self-assemblies, solid-state particle engineering design, organic solution advanced spray dryinghttp://www.dovepress.com/design-and-physicochemical-characterization-of-advanced-spray-dried-ta-a12115 |
| spellingShingle | Wu X Hayes D Jr Zwischenberger JB Kuhn RJ Mansour HM Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation Drug Design, Development and Therapy |
| title | Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation |
| title_full | Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation |
| title_fullStr | Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation |
| title_full_unstemmed | Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation |
| title_short | Design and physicochemical characterization of advanced spray-dried tacrolimus multifunctional particles for inhalation |
| title_sort | design and physicochemical characterization of advanced spray dried tacrolimus multifunctional particles for inhalation |
| url | http://www.dovepress.com/design-and-physicochemical-characterization-of-advanced-spray-dried-ta-a12115 |
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