Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease
Thirteen communities from 7 Argentinian provinces were selected for the evaluation of serology as an indicator of transmission of Chagas disease. Of the communities appraised, 6 did not have a history of previous treatment with insecticides and 7 had received sporadic or continuous insecticide treat...
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Sociedade Brasileira de Medicina Tropical (SBMT)
1989-09-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000300002 |
| _version_ | 1817979068673425408 |
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| author | Roberto Chuit Elisabet Subias Analia C. Pérez Irene Paulone Cristina Wisnivesky-Colli Elsa L. Segura |
| author_facet | Roberto Chuit Elisabet Subias Analia C. Pérez Irene Paulone Cristina Wisnivesky-Colli Elsa L. Segura |
| author_sort | Roberto Chuit |
| collection | DOAJ |
| description | Thirteen communities from 7 Argentinian provinces were selected for the evaluation of serology as an indicator of transmission of Chagas disease. Of the communities appraised, 6 did not have a history of previous treatment with insecticides and 7 had received sporadic or continuous insecticide treatment. The inhabitants of 20% of the houses of each locality were studied by serology. The samples were obtained byfinger pricking and 50 fil of blood were mixed with 150μl of 50% glycerine solution in tissue culture media to be assayed by Indirect Hemagglutination and Indirect Immunofluorescence tests. In untreated areas, the prevalence of infection in infants 0-4 years old was 17.5%, reaching to over 22% for the 5-9 year old group, and to 33.3% in 10-14 year old individuals. The prevalence in treated and surveyed areas was 2.6% in 0-4 year old children, 5.4% in 5-9 year old and 6,2% in 10-14 year old youngsters. The differences between both areas were statistically significant (p < 0.005). This study favors serology as a valid indicator for the evaluation of transmission of Chagas disease in rural areas.<br>Treze comunidades de 7 províncias argentinas foram escolhidas para avaliação de sorologia como indicador da transmissão da Doença de Chagas. Das comunidades mencionadas, seis não tinham história prévia de tratamento com inseticidas e sete tinham recebido tratamento esporádico ou continuado. Vinte por cento dos moradores das casas de cada localidade foram estudados por sorologia. As amostras foram obtidas por punção da ponta do dedo e 50 microlitros de sangue foram misturadas com 150 microlitros de uma solução conservadora de glicerina a 50% em meio de cultivo, para serem estudados por hemaglutinação indireta, e imunofluorescência indireta. Nas áreas não tratadas a prevalência da infecção em crianças de 0-4 anos foi de 17,5% chegando a mais de 22% para as de 5-9 anos e a 33,3% no grupo 10-14 anos. A prevalência nas áreas tratadas e sob vigilância foi de 2.6% em crianças de 0-4 anos, 5,4% anos de 5-9 anos e de 6,2% em jovens de 10-14 sendo as diferenças entre os dois tipos de áreas estatisticamente significativas (< 0,005). Este estudo propõe a sorologia como um indicador válido para a avaliação da transmissão da Doença de Chagas em áreas rurais. |
| first_indexed | 2024-04-13T22:38:11Z |
| format | Article |
| id | doaj.art-64182e1a4efb4a61ae513a8f3a309b96 |
| institution | Directory Open Access Journal |
| issn | 0037-8682 1678-9849 |
| language | English |
| last_indexed | 2024-04-13T22:38:11Z |
| publishDate | 1989-09-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-64182e1a4efb4a61ae513a8f3a309b962022-12-22T02:26:42ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical0037-86821678-98491989-09-01223119124Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' diseaseRoberto ChuitElisabet SubiasAnalia C. PérezIrene PauloneCristina Wisnivesky-ColliElsa L. SeguraThirteen communities from 7 Argentinian provinces were selected for the evaluation of serology as an indicator of transmission of Chagas disease. Of the communities appraised, 6 did not have a history of previous treatment with insecticides and 7 had received sporadic or continuous insecticide treatment. The inhabitants of 20% of the houses of each locality were studied by serology. The samples were obtained byfinger pricking and 50 fil of blood were mixed with 150μl of 50% glycerine solution in tissue culture media to be assayed by Indirect Hemagglutination and Indirect Immunofluorescence tests. In untreated areas, the prevalence of infection in infants 0-4 years old was 17.5%, reaching to over 22% for the 5-9 year old group, and to 33.3% in 10-14 year old individuals. The prevalence in treated and surveyed areas was 2.6% in 0-4 year old children, 5.4% in 5-9 year old and 6,2% in 10-14 year old youngsters. The differences between both areas were statistically significant (p < 0.005). This study favors serology as a valid indicator for the evaluation of transmission of Chagas disease in rural areas.<br>Treze comunidades de 7 províncias argentinas foram escolhidas para avaliação de sorologia como indicador da transmissão da Doença de Chagas. Das comunidades mencionadas, seis não tinham história prévia de tratamento com inseticidas e sete tinham recebido tratamento esporádico ou continuado. Vinte por cento dos moradores das casas de cada localidade foram estudados por sorologia. As amostras foram obtidas por punção da ponta do dedo e 50 microlitros de sangue foram misturadas com 150 microlitros de uma solução conservadora de glicerina a 50% em meio de cultivo, para serem estudados por hemaglutinação indireta, e imunofluorescência indireta. Nas áreas não tratadas a prevalência da infecção em crianças de 0-4 anos foi de 17,5% chegando a mais de 22% para as de 5-9 anos e a 33,3% no grupo 10-14 anos. A prevalência nas áreas tratadas e sob vigilância foi de 2.6% em crianças de 0-4 anos, 5,4% anos de 5-9 anos e de 6,2% em jovens de 10-14 sendo as diferenças entre os dois tipos de áreas estatisticamente significativas (< 0,005). Este estudo propõe a sorologia como um indicador válido para a avaliação da transmissão da Doença de Chagas em áreas rurais.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000300002Transmissão de T. cruziPrevenção e controleEstudo de campoSorodiagnósticoT. cruzi transmissionPrevention and controlField studySerodiagnosis |
| spellingShingle | Roberto Chuit Elisabet Subias Analia C. Pérez Irene Paulone Cristina Wisnivesky-Colli Elsa L. Segura Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease Revista da Sociedade Brasileira de Medicina Tropical Transmissão de T. cruzi Prevenção e controle Estudo de campo Sorodiagnóstico T. cruzi transmission Prevention and control Field study Serodiagnosis |
| title | Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease |
| title_full | Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease |
| title_fullStr | Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease |
| title_full_unstemmed | Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease |
| title_short | Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease |
| title_sort | usefulness of serology for the evaluation of trypanosoma cruzi transmission in endemic areas of chagas disease |
| topic | Transmissão de T. cruzi Prevenção e controle Estudo de campo Sorodiagnóstico T. cruzi transmission Prevention and control Field study Serodiagnosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000300002 |
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