Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional study
Abstract Background Gram-negative bacilli are the most common etiological agents responsible for urinary tract infections. The prevalence of antibiotic resistance in Gram-negative bacilli is increasing at a rapid pace globally, which is constraining the available choices for UTI treatment. The objec...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-024-09023-7 |
| _version_ | 1797275683445014528 |
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| author | Manal Ismail Abdalla Mohammedkheir Elsheikh Mahgoub Gaafar Eltayeb GareebAlla Eltayeb AbdAlla |
| author_facet | Manal Ismail Abdalla Mohammedkheir Elsheikh Mahgoub Gaafar Eltayeb GareebAlla Eltayeb AbdAlla |
| author_sort | Manal Ismail Abdalla Mohammedkheir |
| collection | DOAJ |
| description | Abstract Background Gram-negative bacilli are the most common etiological agents responsible for urinary tract infections. The prevalence of antibiotic resistance in Gram-negative bacilli is increasing at a rapid pace globally, which is constraining the available choices for UTI treatment. The objectives of this study are to identify the most common causal organisms of urinary tract infections (UTIs), and to determine their drug resistance patterns. Materials and methods This was a cross-sectional hospital-based study conducted at El-Amal Hospital, Bahri Teaching Hospital, and Al-Baraha Hospital, Khartoum State, from March to October 2022. Urine samples from patients suspected to have UTI were collected, and patients with confirmed UTI by laboratory investigations and yielded culture growth were enrolled. Antibiotic sensitivity testing and PCR testing of the bla TEM, bla SHV, and bla CTX-M genes were done. Results This study included 50 patients with UTI out of 229 suspected patients (21.8%). The most prominent group of patients was older than 60 years (40%); the majority were females (70%). Escherichia coli was the most prevalent isolated organism (50%), followed by Klebsiella oxytoca (24%), Klebsiella pneumoniae (20%), Pseudomonas aeruginosa (4%), and Citrobacter freundii (2%). A small percentage of organisms were resistant to colistin (17%). However, 77% were resistant to amikacin, 97.6% to cefotaxime, 96.8% to ceftazidime, 97.6% to ceftriaxone, 96.8% to cefixime, 87.6% to ciprofloxacin, 88.4% to gentamycin, 62% to imipenem, 67.6% to meropenem, 87.6% to norfloxacin, and 95.6% to trimethoprim. The overall resistance of isolated gram-negative organisms was 81%. The most prevalent gene for the resistance was bla TEM (100%), followed by bla CTX-M (94%), and then bla SHV (84%). Conclusion Escherichia coli and Klebsiella species were the most commonly isolated uropathogens in this study, and the majority were highly resistant to most of the antimicrobial agents tested. Resistance genes bla TEM, bla CTX-M, and bla SHV are very common in uropathogens. |
| first_indexed | 2024-03-07T15:18:03Z |
| format | Article |
| id | doaj.art-641d71fd95ab482083d0d369c1095fdc |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-03-07T15:18:03Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-641d71fd95ab482083d0d369c1095fdc2024-03-05T17:49:53ZengBMCBMC Infectious Diseases1471-23342024-01-012411710.1186/s12879-024-09023-7Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional studyManal Ismail Abdalla Mohammedkheir0Elsheikh Mahgoub Gaafar1Eltayeb GareebAlla Eltayeb AbdAlla2Sudan International UniversityUniversity of KhartoumUniversity of KhartoumAbstract Background Gram-negative bacilli are the most common etiological agents responsible for urinary tract infections. The prevalence of antibiotic resistance in Gram-negative bacilli is increasing at a rapid pace globally, which is constraining the available choices for UTI treatment. The objectives of this study are to identify the most common causal organisms of urinary tract infections (UTIs), and to determine their drug resistance patterns. Materials and methods This was a cross-sectional hospital-based study conducted at El-Amal Hospital, Bahri Teaching Hospital, and Al-Baraha Hospital, Khartoum State, from March to October 2022. Urine samples from patients suspected to have UTI were collected, and patients with confirmed UTI by laboratory investigations and yielded culture growth were enrolled. Antibiotic sensitivity testing and PCR testing of the bla TEM, bla SHV, and bla CTX-M genes were done. Results This study included 50 patients with UTI out of 229 suspected patients (21.8%). The most prominent group of patients was older than 60 years (40%); the majority were females (70%). Escherichia coli was the most prevalent isolated organism (50%), followed by Klebsiella oxytoca (24%), Klebsiella pneumoniae (20%), Pseudomonas aeruginosa (4%), and Citrobacter freundii (2%). A small percentage of organisms were resistant to colistin (17%). However, 77% were resistant to amikacin, 97.6% to cefotaxime, 96.8% to ceftazidime, 97.6% to ceftriaxone, 96.8% to cefixime, 87.6% to ciprofloxacin, 88.4% to gentamycin, 62% to imipenem, 67.6% to meropenem, 87.6% to norfloxacin, and 95.6% to trimethoprim. The overall resistance of isolated gram-negative organisms was 81%. The most prevalent gene for the resistance was bla TEM (100%), followed by bla CTX-M (94%), and then bla SHV (84%). Conclusion Escherichia coli and Klebsiella species were the most commonly isolated uropathogens in this study, and the majority were highly resistant to most of the antimicrobial agents tested. Resistance genes bla TEM, bla CTX-M, and bla SHV are very common in uropathogens.https://doi.org/10.1186/s12879-024-09023-7Urinary tract infectionsAntibiotic resistanceResistance genes |
| spellingShingle | Manal Ismail Abdalla Mohammedkheir Elsheikh Mahgoub Gaafar Eltayeb GareebAlla Eltayeb AbdAlla Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional study BMC Infectious Diseases Urinary tract infections Antibiotic resistance Resistance genes |
| title | Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional study |
| title_full | Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional study |
| title_fullStr | Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional study |
| title_full_unstemmed | Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional study |
| title_short | Assessment of Bla TEM, Bla SHV, and Bla CTX-M genes of antibiotic resistance in Gram-negative bacilli causing urinary tract infections in Khartoum State: a cross-sectional study |
| title_sort | assessment of bla tem bla shv and bla ctx m genes of antibiotic resistance in gram negative bacilli causing urinary tract infections in khartoum state a cross sectional study |
| topic | Urinary tract infections Antibiotic resistance Resistance genes |
| url | https://doi.org/10.1186/s12879-024-09023-7 |
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