The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formation
Introduction and Objectives: The incidence of gallstone-related disease steadily increased in the last few years. Here, we aimed to investigate the effect of tauroursodeoxycholic acid11 TUDCA: tauroursodeoxycholic acid (TUDCA) on preventing cholesterol gallstones formation in high-fat fed (HFD) mice...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268120302143 |
| _version_ | 1818892546292580352 |
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| author | Qifan Lu Zhaoyan Jiang Qihan Wang Hai Hu Gang Zhao |
| author_facet | Qifan Lu Zhaoyan Jiang Qihan Wang Hai Hu Gang Zhao |
| author_sort | Qifan Lu |
| collection | DOAJ |
| description | Introduction and Objectives: The incidence of gallstone-related disease steadily increased in the last few years. Here, we aimed to investigate the effect of tauroursodeoxycholic acid11 TUDCA: tauroursodeoxycholic acid (TUDCA) on preventing cholesterol gallstones formation in high-fat fed (HFD) mice. Material and Methods: Specific pathogen-free male C57Bl/6 mice were fed a lithogenic diet22 LD: lithogenic diet (LD group) alone or in combination with TUDCA (5 g/kg diet) for 8 weeks. Upon sacrifice, serum, gallbladder, liver and small intestine were collected and the formation of gallstones or crystals in the gallbladder was analyzed. Additionally, the intestinal microbiota, and bile acid composition, serum lipids and hepatic lipids were studied. Results: Cholesterol gallstones with cholesterol crystals formed in mice of the LD-fed group (15/15, 100%). However, only cholesterol crystals were found in three mice without the presence of any gallstone in the TUDCA-treated group. Both serum and hepatic total cholesterol levels in the TUDCA group were significantly decreased compared with the LD group. Concomitantly, mRNA expression of Abcg5 and Abcg8 was significantly lower in the liver of the TUDCA group whilst mRNA transcripts for Abcb11, Acat2, and Cyp27 were significantly increased compared with the LD group. Additionally, the gallbladder cholesterol saturation index (1.06 ± 0.15) in the TUDCA group was significantly decreased compared with the LD group. Interestingly, the ratio of Firmicutes/Bacteroides in the TUDCA group was increased 3x fold. Conclusions: TUDCA can inhibit the absorption and synthesis of lipids in the small intestine by improving the intestinal microbiota in HFD-fed mice, thus reducing gallstone formation. |
| first_indexed | 2024-12-19T17:58:25Z |
| format | Article |
| id | doaj.art-641e6fce769a4b688277d81c80333ff5 |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-19T17:58:25Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-641e6fce769a4b688277d81c80333ff52022-12-21T20:11:44ZengElsevierAnnals of Hepatology1665-26812021-07-0123100289The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formationQifan Lu0Zhaoyan Jiang1Qihan Wang2Hai Hu3Gang Zhao4Tongji University School of Medicine, Shanghai 200092, ChinaCholelithiasis Center, Tongji University Affiliated Shanghai East Hospital, Shanghai 200120, ChinaCholelithiasis Center, Tongji University Affiliated Shanghai East Hospital, Shanghai 200120, ChinaCholelithiasis Center, Tongji University Affiliated Shanghai East Hospital, Shanghai 200120, China; Corresponding author at: Center of Gallbladder Disease, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 20120, ChinaCholelithiasis Center, Tongji University Affiliated Shanghai East Hospital, Shanghai 200120, China; Corresponding author at: Center of Gallbladder Disease, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 20120, ChinaIntroduction and Objectives: The incidence of gallstone-related disease steadily increased in the last few years. Here, we aimed to investigate the effect of tauroursodeoxycholic acid11 TUDCA: tauroursodeoxycholic acid (TUDCA) on preventing cholesterol gallstones formation in high-fat fed (HFD) mice. Material and Methods: Specific pathogen-free male C57Bl/6 mice were fed a lithogenic diet22 LD: lithogenic diet (LD group) alone or in combination with TUDCA (5 g/kg diet) for 8 weeks. Upon sacrifice, serum, gallbladder, liver and small intestine were collected and the formation of gallstones or crystals in the gallbladder was analyzed. Additionally, the intestinal microbiota, and bile acid composition, serum lipids and hepatic lipids were studied. Results: Cholesterol gallstones with cholesterol crystals formed in mice of the LD-fed group (15/15, 100%). However, only cholesterol crystals were found in three mice without the presence of any gallstone in the TUDCA-treated group. Both serum and hepatic total cholesterol levels in the TUDCA group were significantly decreased compared with the LD group. Concomitantly, mRNA expression of Abcg5 and Abcg8 was significantly lower in the liver of the TUDCA group whilst mRNA transcripts for Abcb11, Acat2, and Cyp27 were significantly increased compared with the LD group. Additionally, the gallbladder cholesterol saturation index (1.06 ± 0.15) in the TUDCA group was significantly decreased compared with the LD group. Interestingly, the ratio of Firmicutes/Bacteroides in the TUDCA group was increased 3x fold. Conclusions: TUDCA can inhibit the absorption and synthesis of lipids in the small intestine by improving the intestinal microbiota in HFD-fed mice, thus reducing gallstone formation.http://www.sciencedirect.com/science/article/pii/S1665268120302143Tauroursodeoxycholic acid (TUDCA)High-fat diet (HFD)Intestinal microbiotaCholesterol stonesLipids |
| spellingShingle | Qifan Lu Zhaoyan Jiang Qihan Wang Hai Hu Gang Zhao The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formation Annals of Hepatology Tauroursodeoxycholic acid (TUDCA) High-fat diet (HFD) Intestinal microbiota Cholesterol stones Lipids |
| title | The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formation |
| title_full | The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formation |
| title_fullStr | The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formation |
| title_full_unstemmed | The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formation |
| title_short | The effect of Tauroursodeoxycholic acid (TUDCA) and gut microbiota on murine gallbladder stone formation |
| title_sort | effect of tauroursodeoxycholic acid tudca and gut microbiota on murine gallbladder stone formation |
| topic | Tauroursodeoxycholic acid (TUDCA) High-fat diet (HFD) Intestinal microbiota Cholesterol stones Lipids |
| url | http://www.sciencedirect.com/science/article/pii/S1665268120302143 |
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