The Role of PEMFs on Bone Healing: An In Vitro Study
Bone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/14298 |
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| author | Laura Caliogna Valentina Bina Alice Maria Brancato Giulia Gastaldi Salvatore Annunziata Mario Mosconi Federico Alberto Grassi Francesco Benazzo Gianluigi Pasta |
| author_facet | Laura Caliogna Valentina Bina Alice Maria Brancato Giulia Gastaldi Salvatore Annunziata Mario Mosconi Federico Alberto Grassi Francesco Benazzo Gianluigi Pasta |
| author_sort | Laura Caliogna |
| collection | DOAJ |
| description | Bone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their action. Specifically, we evaluated the effects of PEMFs both on human adipose mesenchymal stem cells (hASCs) and on primary human osteoblasts (hOBs) by testing gene and protein expression of early bone markers (on hASCs) and the synthesis of late bone-specific proteins (on hOBs) as markers of bone remodeling. Our results indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells. Understanding the role of PEMFs on bone regenerative processes provides important details for their clinical application. |
| first_indexed | 2024-03-09T18:15:41Z |
| format | Article |
| id | doaj.art-641f0c3d1cfa4c09b42368e514f920f9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:15:41Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-641f0c3d1cfa4c09b42368e514f920f92023-11-24T08:41:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221429810.3390/ijms232214298The Role of PEMFs on Bone Healing: An In Vitro StudyLaura Caliogna0Valentina Bina1Alice Maria Brancato2Giulia Gastaldi3Salvatore Annunziata4Mario Mosconi5Federico Alberto Grassi6Francesco Benazzo7Gianluigi Pasta8Orthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, 27100 Pavia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyDepartment of Molecular Medicine, University of Pavia, 27100 Pavia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalySezione di Chirurgia Protesica ad Indirizzo Robotico-Unità di Traumatologia dello Sport, U.O. Ortopedia e Traumatologia Fondazione Poliambulanza, 25124 Brescia, ItalyOrthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, ItalyBone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their action. Specifically, we evaluated the effects of PEMFs both on human adipose mesenchymal stem cells (hASCs) and on primary human osteoblasts (hOBs) by testing gene and protein expression of early bone markers (on hASCs) and the synthesis of late bone-specific proteins (on hOBs) as markers of bone remodeling. Our results indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells. Understanding the role of PEMFs on bone regenerative processes provides important details for their clinical application.https://www.mdpi.com/1422-0067/23/22/14298pulsed electromagnetic fields (PEMFs)human adipose mesenchymal stem cells (hASCs)human osteoblasts (hOBs)osteogenic differentiationfracture repairfracture healing |
| spellingShingle | Laura Caliogna Valentina Bina Alice Maria Brancato Giulia Gastaldi Salvatore Annunziata Mario Mosconi Federico Alberto Grassi Francesco Benazzo Gianluigi Pasta The Role of PEMFs on Bone Healing: An In Vitro Study International Journal of Molecular Sciences pulsed electromagnetic fields (PEMFs) human adipose mesenchymal stem cells (hASCs) human osteoblasts (hOBs) osteogenic differentiation fracture repair fracture healing |
| title | The Role of PEMFs on Bone Healing: An In Vitro Study |
| title_full | The Role of PEMFs on Bone Healing: An In Vitro Study |
| title_fullStr | The Role of PEMFs on Bone Healing: An In Vitro Study |
| title_full_unstemmed | The Role of PEMFs on Bone Healing: An In Vitro Study |
| title_short | The Role of PEMFs on Bone Healing: An In Vitro Study |
| title_sort | role of pemfs on bone healing an in vitro study |
| topic | pulsed electromagnetic fields (PEMFs) human adipose mesenchymal stem cells (hASCs) human osteoblasts (hOBs) osteogenic differentiation fracture repair fracture healing |
| url | https://www.mdpi.com/1422-0067/23/22/14298 |
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