PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC
Abstract Chronic wasting disease (CWD) is a prion disease affecting cervids. CWD diagnosis is conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47105-9 |
| _version_ | 1797576835401252864 |
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| author | Rebeca Benavente J. Hunter Reed Mitch Lockwood Rodrigo Morales |
| author_facet | Rebeca Benavente J. Hunter Reed Mitch Lockwood Rodrigo Morales |
| author_sort | Rebeca Benavente |
| collection | DOAJ |
| description | Abstract Chronic wasting disease (CWD) is a prion disease affecting cervids. CWD diagnosis is conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD-prions). Two in vitro prion amplification techniques, real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA), have shown promise in detecting CWD-prions in tissues and bodily fluids. Recent studies have demonstrated that RT-QuIC yields similar results compared to ELISA and IHC. Here, we analyzed 1003 retropharyngeal lymph nodes (RPLNs) from Texas white-tailed deer. PMCA detected CWD at a higher rate compared to ELISA/IHC, identified different prion strains, and revealed the presence of CWD-prions in places with no previous history. These findings suggest that PMCA exhibits greater sensitivity than current standard techniques and could be valuable for rapid and strain-specific CWD detection. |
| first_indexed | 2024-03-10T21:59:20Z |
| format | Article |
| id | doaj.art-6420fafb48a542b9871ef1bd4cc0d5e6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:59:20Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6420fafb48a542b9871ef1bd4cc0d5e62023-11-19T12:59:40ZengNature PortfolioScientific Reports2045-23222023-11-0113111110.1038/s41598-023-47105-9PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHCRebeca Benavente0J. Hunter Reed1Mitch Lockwood2Rodrigo Morales3Department of Neurology, McGovern Medical School, The University of Texas Health Science Center at HoustonTexas Parks and Wildlife DepartmentTexas Parks and Wildlife DepartmentDepartment of Neurology, McGovern Medical School, The University of Texas Health Science Center at HoustonAbstract Chronic wasting disease (CWD) is a prion disease affecting cervids. CWD diagnosis is conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD-prions). Two in vitro prion amplification techniques, real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA), have shown promise in detecting CWD-prions in tissues and bodily fluids. Recent studies have demonstrated that RT-QuIC yields similar results compared to ELISA and IHC. Here, we analyzed 1003 retropharyngeal lymph nodes (RPLNs) from Texas white-tailed deer. PMCA detected CWD at a higher rate compared to ELISA/IHC, identified different prion strains, and revealed the presence of CWD-prions in places with no previous history. These findings suggest that PMCA exhibits greater sensitivity than current standard techniques and could be valuable for rapid and strain-specific CWD detection.https://doi.org/10.1038/s41598-023-47105-9 |
| spellingShingle | Rebeca Benavente J. Hunter Reed Mitch Lockwood Rodrigo Morales PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC Scientific Reports |
| title | PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC |
| title_full | PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC |
| title_fullStr | PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC |
| title_full_unstemmed | PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC |
| title_short | PMCA screening of retropharyngeal lymph nodes in white-tailed deer and comparisons with ELISA and IHC |
| title_sort | pmca screening of retropharyngeal lymph nodes in white tailed deer and comparisons with elisa and ihc |
| url | https://doi.org/10.1038/s41598-023-47105-9 |
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