Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides.
Identification and quantification of immunogenic peptides and tumor-derived epitopes presented on MHC-I molecules are essential for basic studies and vaccines generation. Although lymphocytes derived from transgenic mice can serve as sensitive detectors of processes of antigen presentation and recog...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3562179?pdf=render |
| _version_ | 1818437516476284928 |
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| author | Gal Cafri Adi Sharbi-Yunger Esther Tzehoval Lea Eisenbach |
| author_facet | Gal Cafri Adi Sharbi-Yunger Esther Tzehoval Lea Eisenbach |
| author_sort | Gal Cafri |
| collection | DOAJ |
| description | Identification and quantification of immunogenic peptides and tumor-derived epitopes presented on MHC-I molecules are essential for basic studies and vaccines generation. Although lymphocytes derived from transgenic mice can serve as sensitive detectors of processes of antigen presentation and recognition, they are not always available. The use of cell lines might be extremely useful. In this study, we generated a lacZ inducible CD8⁺ hybridoma (BUSA14) capable of recognizing both human and mouse gp100₂₅₋₃₃ melanoma antigens presented on dendritic and tumor cell lines. This hybridoma expresses a variety of membranal T cell markers and secretes IL-2 and TNFα. Thus, BUSA14 offers a quantifiable, cheap and straightforward tool for studying peptide presentation by MHC-I molecules on the cell surface. |
| first_indexed | 2024-12-14T17:25:55Z |
| format | Article |
| id | doaj.art-642142e78acf409595d13ca39fc69c83 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T17:25:55Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-642142e78acf409595d13ca39fc69c832022-12-21T22:53:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5558310.1371/journal.pone.0055583Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides.Gal CafriAdi Sharbi-YungerEsther TzehovalLea EisenbachIdentification and quantification of immunogenic peptides and tumor-derived epitopes presented on MHC-I molecules are essential for basic studies and vaccines generation. Although lymphocytes derived from transgenic mice can serve as sensitive detectors of processes of antigen presentation and recognition, they are not always available. The use of cell lines might be extremely useful. In this study, we generated a lacZ inducible CD8⁺ hybridoma (BUSA14) capable of recognizing both human and mouse gp100₂₅₋₃₃ melanoma antigens presented on dendritic and tumor cell lines. This hybridoma expresses a variety of membranal T cell markers and secretes IL-2 and TNFα. Thus, BUSA14 offers a quantifiable, cheap and straightforward tool for studying peptide presentation by MHC-I molecules on the cell surface.http://europepmc.org/articles/PMC3562179?pdf=render |
| spellingShingle | Gal Cafri Adi Sharbi-Yunger Esther Tzehoval Lea Eisenbach Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. PLoS ONE |
| title | Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. |
| title_full | Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. |
| title_fullStr | Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. |
| title_full_unstemmed | Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. |
| title_short | Production of LacZ inducible T cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides. |
| title_sort | production of lacz inducible t cell hybridoma specific for human and mouse gp100₂₅₋₃₃ peptides |
| url | http://europepmc.org/articles/PMC3562179?pdf=render |
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