Development of bipolar pulse generator for cell electroporation
BackgroundCell electroporation technology under pulsed electric field has been widely used in water treatment, food sterilization, tissue ablation and other applications. Different electroporation applications have different requirements for the pulse waveform.PurposeThis study aims to propose a new...
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| Format: | Article |
| Language: | zho |
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Science Press
2021-10-01
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| Series: | He jishu |
| Subjects: | |
| Online Access: | http://www.hjs.sinap.ac.cn/thesisDetails#10.11889/j.0253-3219.2021.hjs.44.100401&lang=zh |
| _version_ | 1811169622277226496 |
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| author | WU Zhonghang SHI Haozhi JIANG Song RAO Junfeng |
| author_facet | WU Zhonghang SHI Haozhi JIANG Song RAO Junfeng |
| author_sort | WU Zhonghang |
| collection | DOAJ |
| description | BackgroundCell electroporation technology under pulsed electric field has been widely used in water treatment, food sterilization, tissue ablation and other applications. Different electroporation applications have different requirements for the pulse waveform.PurposeThis study aims to propose a new pulse generator structure consisting of a special full bridge (FB) module and multiple half bridge (HB) modules to generate bipolar pulse voltages, stepped waves and combination of narrow and wide pulses can also be realized by only changing the switching sequence.MethodsFirst of all, the structure and working principle of novel circuit were elaborated. Then, a main circuit structure composed of 1 full-bridge module and 4 half-bridge modules composed of 12 switches were designed and implemented. Bipolar pulse voltages, stepped waves and combination of narrow and wide pulses were realized by only changing the switching sequence. Finally, verification of the power supply for cell electroporation experiments was carried out in the potato tuber treatment experiment. [ResultsandConclusions] When each level of capacitor is charged at 220 V, bipolar pulse voltage output with ±2 kV and adjustable pulse width of 1~10 μs is realized. Furthermore, the output of a unipolar 9-level step voltage and a combination of wide and narrow pulses are achieved by controlling the switching sequence. Experiment of potato tuber treatment verified the feasibility of this pulse generator. |
| first_indexed | 2024-04-10T16:46:30Z |
| format | Article |
| id | doaj.art-64246ada698f41fb8cdc0cf21ad3accd |
| institution | Directory Open Access Journal |
| issn | 0253-3219 |
| language | zho |
| last_indexed | 2024-04-10T16:46:30Z |
| publishDate | 2021-10-01 |
| publisher | Science Press |
| record_format | Article |
| series | He jishu |
| spelling | doaj.art-64246ada698f41fb8cdc0cf21ad3accd2023-02-08T00:42:07ZzhoScience PressHe jishu0253-32192021-10-014410172210.11889/j.0253-3219.2021.hjs.44.1004010253-3219（2021）10-0017-06Development of bipolar pulse generator for cell electroporationWU Zhonghang0SHI Haozhi1JIANG Song2RAO Junfeng3Technology Innovation Platform of Zhoupu Hospital Affiliated to Shanghai Medical College, Shanghai 201318, ChinaSchool of Mechanical Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Mechanical Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Mechanical Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaBackgroundCell electroporation technology under pulsed electric field has been widely used in water treatment, food sterilization, tissue ablation and other applications. Different electroporation applications have different requirements for the pulse waveform.PurposeThis study aims to propose a new pulse generator structure consisting of a special full bridge (FB) module and multiple half bridge (HB) modules to generate bipolar pulse voltages, stepped waves and combination of narrow and wide pulses can also be realized by only changing the switching sequence.MethodsFirst of all, the structure and working principle of novel circuit were elaborated. Then, a main circuit structure composed of 1 full-bridge module and 4 half-bridge modules composed of 12 switches were designed and implemented. Bipolar pulse voltages, stepped waves and combination of narrow and wide pulses were realized by only changing the switching sequence. Finally, verification of the power supply for cell electroporation experiments was carried out in the potato tuber treatment experiment. [ResultsandConclusions] When each level of capacitor is charged at 220 V, bipolar pulse voltage output with ±2 kV and adjustable pulse width of 1~10 μs is realized. Furthermore, the output of a unipolar 9-level step voltage and a combination of wide and narrow pulses are achieved by controlling the switching sequence. Experiment of potato tuber treatment verified the feasibility of this pulse generator.http://www.hjs.sinap.ac.cn/thesisDetails#10.11889/j.0253-3219.2021.hjs.44.100401&lang=zhpulse generatorbipolarstep waveelectroporation |
| spellingShingle | WU Zhonghang SHI Haozhi JIANG Song RAO Junfeng Development of bipolar pulse generator for cell electroporation He jishu pulse generator bipolar step wave electroporation |
| title | Development of bipolar pulse generator for cell electroporation |
| title_full | Development of bipolar pulse generator for cell electroporation |
| title_fullStr | Development of bipolar pulse generator for cell electroporation |
| title_full_unstemmed | Development of bipolar pulse generator for cell electroporation |
| title_short | Development of bipolar pulse generator for cell electroporation |
| title_sort | development of bipolar pulse generator for cell electroporation |
| topic | pulse generator bipolar step wave electroporation |
| url | http://www.hjs.sinap.ac.cn/thesisDetails#10.11889/j.0253-3219.2021.hjs.44.100401&lang=zh |
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