P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITION
Introduction: Endovascular trophoblasts (EVasT) of the rat express smooth muscle (SM) proteins and contract ex vivo upon exposure to endothelin-1 (ET1). Contraction is mediated via ET1 receptors A and B (ETA, ETB). In vascular SM ETB, in variance from ETA, exerts relaxation through activation of nit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2015-11-01
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| Series: | Artery Research |
| Online Access: | https://www.atlantis-press.com/article/125930604/view |
| _version_ | 1811335891762806784 |
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| author | Ilana Ariel Galina Skarzinski Vitali Belzer Wiessam Abu-Ahmad Zaid Abassi Michael Bursztyn* |
| author_facet | Ilana Ariel Galina Skarzinski Vitali Belzer Wiessam Abu-Ahmad Zaid Abassi Michael Bursztyn* |
| author_sort | Ilana Ariel |
| collection | DOAJ |
| description | Introduction: Endovascular trophoblasts (EVasT) of the rat express smooth muscle (SM) proteins and contract ex vivo upon exposure to endothelin-1 (ET1). Contraction is mediated via ET1 receptors A and B (ETA, ETB). In vascular SM ETB, in variance from ETA, exerts relaxation through activation of nitric oxide synthase (NOS). We investigated the role of NOS expressed by EVasT in reaction to ET1 exposure.
M&M: Cut surface area of remodeled spiral artery rings devoid of SM was measured ex vivo exposed to (a) L-NAME alone, (b) L-NAME and ET1 representing the combined contractile effect of both receptors, and (c) L-NAME with ET1 and ETA antagonist, representing the isolated contractile effect mediated by ETB. These curves were compared with ET1-induced contraction in the presence of receptor antagonists without L-NAME. Statistical analysis was performed 2-way mixed ANOVA.
Results: L-NAME alone reduced lumen cut surface area by 2.2±0.3.% (p=0.002). ET1+L-NAME, representing the sum of constrictive effect via ETA and ETB reduced vascular lumen area immediately, compared with a plateau at 60min by addition of ET1 alone, p=0.004. ET1 + ETA inhibitor + L-NAME, representing the isolated constrictive effect of ETB (5.9±0.6%), demonstrated similar vasoconstriction via ETA (5.3±0.5%) (p=0.018).
Conclusions: EVasT of the rat remodeled spiral artery react to ET1 exposure similar to vascular SM of non-modified arteries: contract via ETA and ETB and relax via ETB through NOS activation. This phenomenon may play a role in situations of dysregulation of the vasoactive systems in rat models of preeclampsia and IUGR. |
| first_indexed | 2024-04-13T17:30:37Z |
| format | Article |
| id | doaj.art-642491e24e4743d79d1c7382f9e0e76b |
| institution | Directory Open Access Journal |
| issn | 1876-4401 |
| language | English |
| last_indexed | 2024-04-13T17:30:37Z |
| publishDate | 2015-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Artery Research |
| spelling | doaj.art-642491e24e4743d79d1c7382f9e0e76b2022-12-22T02:37:35ZengBMCArtery Research1876-44012015-11-011210.1016/j.artres.2015.10.260P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITIONIlana ArielGalina SkarzinskiVitali BelzerWiessam Abu-AhmadZaid AbassiMichael Bursztyn*Introduction: Endovascular trophoblasts (EVasT) of the rat express smooth muscle (SM) proteins and contract ex vivo upon exposure to endothelin-1 (ET1). Contraction is mediated via ET1 receptors A and B (ETA, ETB). In vascular SM ETB, in variance from ETA, exerts relaxation through activation of nitric oxide synthase (NOS). We investigated the role of NOS expressed by EVasT in reaction to ET1 exposure. M&M: Cut surface area of remodeled spiral artery rings devoid of SM was measured ex vivo exposed to (a) L-NAME alone, (b) L-NAME and ET1 representing the combined contractile effect of both receptors, and (c) L-NAME with ET1 and ETA antagonist, representing the isolated contractile effect mediated by ETB. These curves were compared with ET1-induced contraction in the presence of receptor antagonists without L-NAME. Statistical analysis was performed 2-way mixed ANOVA. Results: L-NAME alone reduced lumen cut surface area by 2.2±0.3.% (p=0.002). ET1+L-NAME, representing the sum of constrictive effect via ETA and ETB reduced vascular lumen area immediately, compared with a plateau at 60min by addition of ET1 alone, p=0.004. ET1 + ETA inhibitor + L-NAME, representing the isolated constrictive effect of ETB (5.9±0.6%), demonstrated similar vasoconstriction via ETA (5.3±0.5%) (p=0.018). Conclusions: EVasT of the rat remodeled spiral artery react to ET1 exposure similar to vascular SM of non-modified arteries: contract via ETA and ETB and relax via ETB through NOS activation. This phenomenon may play a role in situations of dysregulation of the vasoactive systems in rat models of preeclampsia and IUGR.https://www.atlantis-press.com/article/125930604/view |
| spellingShingle | Ilana Ariel Galina Skarzinski Vitali Belzer Wiessam Abu-Ahmad Zaid Abassi Michael Bursztyn* P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITION Artery Research |
| title | P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITION |
| title_full | P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITION |
| title_fullStr | P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITION |
| title_full_unstemmed | P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITION |
| title_short | P4.16 THE MYOTROPHOBLAST OF THE RAT PLACENTA: EX VIVO STUDY OF NITRIC OXIDE SYNTHASE INHIBITION |
| title_sort | p4 16 the myotrophoblast of the rat placenta ex vivo study of nitric oxide synthase inhibition |
| url | https://www.atlantis-press.com/article/125930604/view |
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