High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject
BACKGROUND: Premature Rupture of Membranes (PROM) is significantly linked to the infections-related maternal deaths. In the inflammatory process, the influencing stressor will stimulate the activation of Nuclear Factor Kappa B (NF-κB) and Receptor of Advanced Gyclation End product (RAGE). Yet up to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Secretariat of The Indonesian Biomedical Journal
2022-09-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/1859 |
| _version_ | 1798001808961961984 |
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| author | I Ketut Edy Sudiarta Monicha Zalzabilla Aldinasyah Cindy Jennilyn Candra Supriyono Supriyono Annisa Ullya Rasyida |
| author_facet | I Ketut Edy Sudiarta Monicha Zalzabilla Aldinasyah Cindy Jennilyn Candra Supriyono Supriyono Annisa Ullya Rasyida |
| author_sort | I Ketut Edy Sudiarta |
| collection | DOAJ |
| description | BACKGROUND: Premature Rupture of Membranes (PROM) is significantly linked to the infections-related maternal deaths. In the inflammatory process, the influencing stressor will stimulate the activation of Nuclear Factor Kappa B (NF-κB) and Receptor of Advanced Gyclation End product (RAGE). Yet up to date, the expression of NF-κB and RAGE in pregnant women with PROM are still rarely studied. Therefore, this study aimed to observe the differences of NF-κB and RAGE expression from PROM and non-PROM subjects.
METHODS: This was a cross-sectional study involving 20 PROM subjects and 20 non-PROM subjects with infections and complications. Samples from the fetal membrane tissue of subjects were obtained and put into paraffin block preparation for the determination of NF-κB and RAGE expression. The detection of NF-κB and RAGE expression was conducted using immunohistochemical staining and observed under an upright light microscope. The expressions were later calculated using ImageJ software.
RESULTS: Both NF-κB and RAGE expression were found to be higher in PROM subjects compare to the non-PROM subjects. The median of NF-κB in PROM and non-PROM subjects were 32.47±1.22 and 5.59±1.09, respectively (p=0.000). While the median of RAGE in PROM subjects was 53.58±3.46, and in non-PROM subjects was 11.64±2.49 (p=0.013).
CONCLUSION: There is significant difference between NF-κB and RAGE expression in fetal membranes of PROM and non-PROM subjects. Therefore, the increased of NF-κB and RAGE expression can be used as a potential marker to detect complication of PROM.
KEYWORDS: premature ruptures of membrane, non-premature ruptures of membrane, expression of NF-κB |
| first_indexed | 2024-04-11T11:43:18Z |
| format | Article |
| id | doaj.art-6425e54a62d64e99ac39706a2ff3ba03 |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-04-11T11:43:18Z |
| publishDate | 2022-09-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj.art-6425e54a62d64e99ac39706a2ff3ba032022-12-22T04:25:46ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792022-09-011432899310.18585/inabj.v14i3.1859472High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) SubjectI Ketut Edy Sudiarta0Monicha Zalzabilla Aldinasyah1Cindy Jennilyn Candra2Supriyono Supriyono3Annisa Ullya Rasyida4Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Hang Tuah, Jl. Gadung No. 1, SurabayaFaculty of Medicine, Universitas Hang Tuah, Jl. Gadung No. 1, SurabayaFaculty of Medicine, Universitas Hang Tuah, Jl. Gadung No. 1, SurabayaMother and Child Hospital Pondok Tjandra, Jl. Mangga I No.E-225, SidoarjoDepartment of Public Health, Universitas Hang Tuah, Jl. Gadung No. 1, SurabayaBACKGROUND: Premature Rupture of Membranes (PROM) is significantly linked to the infections-related maternal deaths. In the inflammatory process, the influencing stressor will stimulate the activation of Nuclear Factor Kappa B (NF-κB) and Receptor of Advanced Gyclation End product (RAGE). Yet up to date, the expression of NF-κB and RAGE in pregnant women with PROM are still rarely studied. Therefore, this study aimed to observe the differences of NF-κB and RAGE expression from PROM and non-PROM subjects. METHODS: This was a cross-sectional study involving 20 PROM subjects and 20 non-PROM subjects with infections and complications. Samples from the fetal membrane tissue of subjects were obtained and put into paraffin block preparation for the determination of NF-κB and RAGE expression. The detection of NF-κB and RAGE expression was conducted using immunohistochemical staining and observed under an upright light microscope. The expressions were later calculated using ImageJ software. RESULTS: Both NF-κB and RAGE expression were found to be higher in PROM subjects compare to the non-PROM subjects. The median of NF-κB in PROM and non-PROM subjects were 32.47±1.22 and 5.59±1.09, respectively (p=0.000). While the median of RAGE in PROM subjects was 53.58±3.46, and in non-PROM subjects was 11.64±2.49 (p=0.013). CONCLUSION: There is significant difference between NF-κB and RAGE expression in fetal membranes of PROM and non-PROM subjects. Therefore, the increased of NF-κB and RAGE expression can be used as a potential marker to detect complication of PROM. KEYWORDS: premature ruptures of membrane, non-premature ruptures of membrane, expression of NF-κBhttps://inabj.org/index.php/ibj/article/view/1859 |
| spellingShingle | I Ketut Edy Sudiarta Monicha Zalzabilla Aldinasyah Cindy Jennilyn Candra Supriyono Supriyono Annisa Ullya Rasyida High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject Indonesian Biomedical Journal |
| title | High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject |
| title_full | High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject |
| title_fullStr | High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject |
| title_full_unstemmed | High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject |
| title_short | High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject |
| title_sort | high nf κb and rage expression in fetal membrane of premature rupture of membrane prom subject |
| url | https://inabj.org/index.php/ibj/article/view/1859 |
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