Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern Iran
Abstract Background Trichobilharzia regenti (T. regenti) is an avian schistosomatid fluke species that causes human cercarial dermatitis (HCD) in areas of aquaculture in northern Iran. Understanding the phylogenetic relationships and genetic diversity of this thread‐like fluke will deepen our though...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.1225 |
| _version_ | 1827813948118794240 |
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| author | Elham Kia Lashaki Arezoo Bozorgomid Shirzad Gholami Mehdi Karamian Mahdi Fakhar Samira Dodangeh |
| author_facet | Elham Kia Lashaki Arezoo Bozorgomid Shirzad Gholami Mehdi Karamian Mahdi Fakhar Samira Dodangeh |
| author_sort | Elham Kia Lashaki |
| collection | DOAJ |
| description | Abstract Background Trichobilharzia regenti (T. regenti) is an avian schistosomatid fluke species that causes human cercarial dermatitis (HCD) in areas of aquaculture in northern Iran. Understanding the phylogenetic relationships and genetic diversity of this thread‐like fluke will deepen our thoughtful of avian schistosomiasis epidemiology and lead to more effective HCD control in the region. Objectives To determine the life cycle of nasal Trichobilharzia in aquatic birds as well as aquatic snails and also identify the haplotype diversity of the isolates in Mazandaran Province, northern Iran. Methods In the present study, adult or egg of Trichobilharzia isolated from aquatic birds as well as schistosomes cercariae isolated from aquatic snails in Mazandaran Province, northern Iran, belonged to the authors' previous research, were examined. Molecular studies and phylogenetic analysis were carried out on these schistosomes samples. Results The phylogenetic analysis of the ITS1 and COX1 genes in isolated schistosomes revealed that all samples belong to the T. regenti clade. Remarkably, based on phylogenetic results, these schistosomes samples from Anas platyrhynchos domesticus, A. platyrhynchos, Spatula clypeata and Lymnaea stagnalis grouped together with previously sequenced samples from Iran (Trichobilharzia cf. regenti). Unlike the phylogenetic tree and haplotype network of COX1 gene, ITS1 did not show distinct clusters. Conclusion This study completed the puzzle of the disease in Mazandaran Province by isolating and genotyping furkocercariae from L. stagnalis that was consistent with the isolated new genotype from ducks. For the first time in Iran, this confirmed the potential role of L. stagnalis snails in the transmission of the disease. |
| first_indexed | 2024-03-11T23:38:52Z |
| format | Article |
| id | doaj.art-6427517d90834091b0f698c743d3112e |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-11T23:38:52Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-6427517d90834091b0f698c743d3112e2023-09-19T16:49:28ZengWileyVeterinary Medicine and Science2053-10952023-09-01952359236710.1002/vms3.1225Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern IranElham Kia Lashaki0Arezoo Bozorgomid1Shirzad Gholami2Mehdi Karamian3Mahdi Fakhar4Samira Dodangeh5Department of Parasitology and Mycology School of Medicine, Tonekabon Branch, Islamic Azad University TonekabonIranInfectious Diseases Research Center Health Institute Kermanshah University of Medical Sciences KermanshahIranToxoplasmosis Research Center Iranian National Registry Center for Lophomoniasis and Toxoplasmosis School of Medicine, Mazandaran University of Medical Sciences SariIranDepartment of Parasitology and Mycology School of Medicine, Zanjan University of Medical Sciences ZanjanIranToxoplasmosis Research Center Iranian National Registry Center for Lophomoniasis and Toxoplasmosis School of Medicine, Mazandaran University of Medical Sciences SariIranChildren Growth Research Center Research Institute for Prevention of Non‐Communicable Diseases, Qazvin University of Medical Sciences QazvinIranAbstract Background Trichobilharzia regenti (T. regenti) is an avian schistosomatid fluke species that causes human cercarial dermatitis (HCD) in areas of aquaculture in northern Iran. Understanding the phylogenetic relationships and genetic diversity of this thread‐like fluke will deepen our thoughtful of avian schistosomiasis epidemiology and lead to more effective HCD control in the region. Objectives To determine the life cycle of nasal Trichobilharzia in aquatic birds as well as aquatic snails and also identify the haplotype diversity of the isolates in Mazandaran Province, northern Iran. Methods In the present study, adult or egg of Trichobilharzia isolated from aquatic birds as well as schistosomes cercariae isolated from aquatic snails in Mazandaran Province, northern Iran, belonged to the authors' previous research, were examined. Molecular studies and phylogenetic analysis were carried out on these schistosomes samples. Results The phylogenetic analysis of the ITS1 and COX1 genes in isolated schistosomes revealed that all samples belong to the T. regenti clade. Remarkably, based on phylogenetic results, these schistosomes samples from Anas platyrhynchos domesticus, A. platyrhynchos, Spatula clypeata and Lymnaea stagnalis grouped together with previously sequenced samples from Iran (Trichobilharzia cf. regenti). Unlike the phylogenetic tree and haplotype network of COX1 gene, ITS1 did not show distinct clusters. Conclusion This study completed the puzzle of the disease in Mazandaran Province by isolating and genotyping furkocercariae from L. stagnalis that was consistent with the isolated new genotype from ducks. For the first time in Iran, this confirmed the potential role of L. stagnalis snails in the transmission of the disease.https://doi.org/10.1002/vms3.1225aquatic birdsaquatic snailsCOX1IranITS1phylogenetic analysis |
| spellingShingle | Elham Kia Lashaki Arezoo Bozorgomid Shirzad Gholami Mehdi Karamian Mahdi Fakhar Samira Dodangeh Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern Iran Veterinary Medicine and Science aquatic birds aquatic snails COX1 Iran ITS1 phylogenetic analysis |
| title | Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern Iran |
| title_full | Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern Iran |
| title_fullStr | Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern Iran |
| title_full_unstemmed | Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern Iran |
| title_short | Phylogenetic analysis of avian schistosome Trichobilharzia regenti (Schistosomatidae, Digenea) from naturally infected hosts in northern Iran |
| title_sort | phylogenetic analysis of avian schistosome trichobilharzia regenti schistosomatidae digenea from naturally infected hosts in northern iran |
| topic | aquatic birds aquatic snails COX1 Iran ITS1 phylogenetic analysis |
| url | https://doi.org/10.1002/vms3.1225 |
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