The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose.
The spotted wing drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae), a pest of berries stone fruits, invaded North America and Europe in 2008. Current control methods rely mainly on insecticides. The sterile insect technique (SIT) has potential as an additional control tactic for the i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5619704?pdf=render |
| _version_ | 1819122844996468736 |
|---|---|
| author | Geneviève Lanouette Jacques Brodeur François Fournier Véronique Martel Marc Vreysen Carlos Cáceres Annabelle Firlej |
| author_facet | Geneviève Lanouette Jacques Brodeur François Fournier Véronique Martel Marc Vreysen Carlos Cáceres Annabelle Firlej |
| author_sort | Geneviève Lanouette |
| collection | DOAJ |
| description | The spotted wing drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae), a pest of berries stone fruits, invaded North America and Europe in 2008. Current control methods rely mainly on insecticides. The sterile insect technique (SIT) has potential as an additional control tactic for the integrated management of D. suzukii. As a step towards the development of the SIT, this study aimed at finding the optimum irradiation dose to sterilize D. suzukii under controlled laboratory conditions. Four-day-old D. suzukii pupae were irradiated 12 to 24 hours prior to adult emergence in a 60Co Gamma Cell 220 and in a 137Cs Gamma Cell 3000 with doses of 30, 50, 70, 80, 90, 100 or 120 Gy. Emergence rate (88.1%), percent of deformed flies (4.0%) and survival curves were not affected by the tested irradiation doses. However, some reproductive parameters of the flies were affected by irradiation. Females irradiated with a dose of 50 Gy or more had almost no fecundity. When non-irradiated females were mated with irradiated males, egg hatch decreased exponentially with irradiation dose from 82.6% for the untreated control males to 4.0% for males irradiated with 120 Gy. Mortality of F1 individuals from the irradiated treatment also occurred during larval and pupal stages, with an egg to adult survival of 0.2%. However, descendants produced by the irradiated generation were fertile. These results are an encouraging first experimental step towards the development of the SIT for the management of D. suzukii populations. |
| first_indexed | 2024-12-22T06:58:55Z |
| format | Article |
| id | doaj.art-642c957ea32d488b83d69d029b52acf7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T06:58:55Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-642c957ea32d488b83d69d029b52acf72022-12-21T18:34:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018082110.1371/journal.pone.0180821The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose.Geneviève LanouetteJacques BrodeurFrançois FournierVéronique MartelMarc VreysenCarlos CáceresAnnabelle FirlejThe spotted wing drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae), a pest of berries stone fruits, invaded North America and Europe in 2008. Current control methods rely mainly on insecticides. The sterile insect technique (SIT) has potential as an additional control tactic for the integrated management of D. suzukii. As a step towards the development of the SIT, this study aimed at finding the optimum irradiation dose to sterilize D. suzukii under controlled laboratory conditions. Four-day-old D. suzukii pupae were irradiated 12 to 24 hours prior to adult emergence in a 60Co Gamma Cell 220 and in a 137Cs Gamma Cell 3000 with doses of 30, 50, 70, 80, 90, 100 or 120 Gy. Emergence rate (88.1%), percent of deformed flies (4.0%) and survival curves were not affected by the tested irradiation doses. However, some reproductive parameters of the flies were affected by irradiation. Females irradiated with a dose of 50 Gy or more had almost no fecundity. When non-irradiated females were mated with irradiated males, egg hatch decreased exponentially with irradiation dose from 82.6% for the untreated control males to 4.0% for males irradiated with 120 Gy. Mortality of F1 individuals from the irradiated treatment also occurred during larval and pupal stages, with an egg to adult survival of 0.2%. However, descendants produced by the irradiated generation were fertile. These results are an encouraging first experimental step towards the development of the SIT for the management of D. suzukii populations.http://europepmc.org/articles/PMC5619704?pdf=render |
| spellingShingle | Geneviève Lanouette Jacques Brodeur François Fournier Véronique Martel Marc Vreysen Carlos Cáceres Annabelle Firlej The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose. PLoS ONE |
| title | The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose. |
| title_full | The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose. |
| title_fullStr | The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose. |
| title_full_unstemmed | The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose. |
| title_short | The sterile insect technique for the management of the spotted wing drosophila, Drosophila suzukii: Establishing the optimum irradiation dose. |
| title_sort | sterile insect technique for the management of the spotted wing drosophila drosophila suzukii establishing the optimum irradiation dose |
| url | http://europepmc.org/articles/PMC5619704?pdf=render |
| work_keys_str_mv | AT genevievelanouette thesterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT jacquesbrodeur thesterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT francoisfournier thesterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT veroniquemartel thesterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT marcvreysen thesterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT carloscaceres thesterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT annabellefirlej thesterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT genevievelanouette sterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT jacquesbrodeur sterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT francoisfournier sterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT veroniquemartel sterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT marcvreysen sterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT carloscaceres sterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose AT annabellefirlej sterileinsecttechniqueforthemanagementofthespottedwingdrosophiladrosophilasuzukiiestablishingtheoptimumirradiationdose |