Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria
Crude sophorolipids (SLs) have been proven to perform varying degrees of inhibitory effects on different pathogenic bacteria. However, systematic comparative studies of pure lactonic sophorolipid (LSL) among different types of bacteria are few. In this study, the antibacterial effects and mechanisms...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.929932/full |
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| author | Xiao-jing Ma Xiao-jing Ma Tong Wang Hui-min Zhang Jun-qian Shao Mei Jiang Huai Wang Huai Wang Hui-xia Zhu Hui-xia Zhu Dong Zhou |
| author_facet | Xiao-jing Ma Xiao-jing Ma Tong Wang Hui-min Zhang Jun-qian Shao Mei Jiang Huai Wang Huai Wang Hui-xia Zhu Hui-xia Zhu Dong Zhou |
| author_sort | Xiao-jing Ma |
| collection | DOAJ |
| description | Crude sophorolipids (SLs) have been proven to perform varying degrees of inhibitory effects on different pathogenic bacteria. However, systematic comparative studies of pure lactonic sophorolipid (LSL) among different types of bacteria are few. In this study, the antibacterial effects and mechanisms of LSL on pathogenic bacteria of Staphylococcus aureus, Lactobacillus sp., Pseudomonas aeruginosa, and Escherichia coli were investigated. Bacteriostatic circle, antibacterial rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of LSL on different pathogenic bacteria were measured. Then, the antibacterial mechanisms of LSL on S. aureus and P. aeruginosa were explored using ultrastructural observation, cell membrane permeability analysis, intracellular ATP content determination, and extracellular UV absorption detection. With the minimum MIC and MBC values of 0.05 and 0.20 mg/ml, LSL exhibited the best inhibitory effect against S. aureus, followed by P. aeruginosa. LSL showed no significant inhibitory effect on E. coli and Lactobacillus sp. For both S. aureus and P. aeruginosa, LSL achieved bacteriostatic and bactericidal effects by destroying the cell wall, increasing the permeability of the cell membrane and leading to the flow out of intracellular contents. However, the action mode and action intensity of LSL on the cell wall and membrane of these two bacteria were significantly different. LSL had a greater influence on the cell membrane of S. aureus by “leaking,” while it exhibited a stronger effect on the cell wall of P. aeruginosa by “blasting.” These results contributed to a better understanding of the relationship between LSL and different bacterial cell structures, further suggesting the conclusion that LSL might be used for the targeted treatment of special pathogenic bacteria. |
| first_indexed | 2024-04-12T04:24:30Z |
| format | Article |
| id | doaj.art-642d644fe0a3437da32e6b5c14a13770 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T04:24:30Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-642d644fe0a3437da32e6b5c14a137702022-12-22T03:48:07ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.929932929932Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteriaXiao-jing Ma0Xiao-jing Ma1Tong Wang2Hui-min Zhang3Jun-qian Shao4Mei Jiang5Huai Wang6Huai Wang7Hui-xia Zhu8Hui-xia Zhu9Dong Zhou10School of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaMinistry of Education, Engineering Research Center of Bio-Process, Hefei University of Technology, Hefei, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaMinistry of Education, Engineering Research Center of Bio-Process, Hefei University of Technology, Hefei, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, ChinaMinistry of Education, Engineering Research Center of Bio-Process, Hefei University of Technology, Hefei, ChinaDepartment of Pediatrics, Qilu Hospital of Shandong University, Jinan, ChinaCrude sophorolipids (SLs) have been proven to perform varying degrees of inhibitory effects on different pathogenic bacteria. However, systematic comparative studies of pure lactonic sophorolipid (LSL) among different types of bacteria are few. In this study, the antibacterial effects and mechanisms of LSL on pathogenic bacteria of Staphylococcus aureus, Lactobacillus sp., Pseudomonas aeruginosa, and Escherichia coli were investigated. Bacteriostatic circle, antibacterial rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of LSL on different pathogenic bacteria were measured. Then, the antibacterial mechanisms of LSL on S. aureus and P. aeruginosa were explored using ultrastructural observation, cell membrane permeability analysis, intracellular ATP content determination, and extracellular UV absorption detection. With the minimum MIC and MBC values of 0.05 and 0.20 mg/ml, LSL exhibited the best inhibitory effect against S. aureus, followed by P. aeruginosa. LSL showed no significant inhibitory effect on E. coli and Lactobacillus sp. For both S. aureus and P. aeruginosa, LSL achieved bacteriostatic and bactericidal effects by destroying the cell wall, increasing the permeability of the cell membrane and leading to the flow out of intracellular contents. However, the action mode and action intensity of LSL on the cell wall and membrane of these two bacteria were significantly different. LSL had a greater influence on the cell membrane of S. aureus by “leaking,” while it exhibited a stronger effect on the cell wall of P. aeruginosa by “blasting.” These results contributed to a better understanding of the relationship between LSL and different bacterial cell structures, further suggesting the conclusion that LSL might be used for the targeted treatment of special pathogenic bacteria.https://www.frontiersin.org/articles/10.3389/fmicb.2022.929932/fulllactonic sophorolipidpathogenic bacteriaantibacterial effectantibacterial mechanismStaphylococcus aureusPseudomonas aeruginosa |
| spellingShingle | Xiao-jing Ma Xiao-jing Ma Tong Wang Hui-min Zhang Jun-qian Shao Mei Jiang Huai Wang Huai Wang Hui-xia Zhu Hui-xia Zhu Dong Zhou Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria Frontiers in Microbiology lactonic sophorolipid pathogenic bacteria antibacterial effect antibacterial mechanism Staphylococcus aureus Pseudomonas aeruginosa |
| title | Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria |
| title_full | Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria |
| title_fullStr | Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria |
| title_full_unstemmed | Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria |
| title_short | Comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria |
| title_sort | comparison of inhibitory effects and mechanisms of lactonic sophorolipid on different pathogenic bacteria |
| topic | lactonic sophorolipid pathogenic bacteria antibacterial effect antibacterial mechanism Staphylococcus aureus Pseudomonas aeruginosa |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.929932/full |
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