Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique.
Colonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/ week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /specie...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2017-06-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4302 |
| _version_ | 1797364015626715136 |
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| author | Najm Hadi Najim Amer Jebur Obayes AL-Isawi |
| author_facet | Najm Hadi Najim Amer Jebur Obayes AL-Isawi |
| author_sort | Najm Hadi Najim |
| collection | DOAJ |
| description | Colonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/ week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /species/season) where the first periad was extended from the begining of December 2015 to the end of February 2016 while the second period was extended from the begining of July to the end of September 2016.The identification of E.Coli O157:H7 isolates were confirmed based on their cultural, biochemical and seralogical characteristics using the commercial latex agglutination test kit and by duplex PCR technique. Data revealed that there was a significant (P≤ 0.05) seasonal variation in the prevalence of E.Coli O157:H7 where all Cow's and Buffalo's soft cheese samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer season (50% and 40% respectively) than in winter season (25% and 15% respectively).It was found that all of the 80 bovine soft cheese samples had significantly (p<0.05) higher prevalence of E.Coli O157:H7 in summer (45%) than in winter (20%) seasons.
The results of the diagnosis of E.coli O157:H7 were confirmed by PCR assay which showed that,six culturing isolates serotype E.coli O157:H7 were postive by PCR assay and five of these isolates expressed gene of Stx1 whearse one isolate expressed gene of Stx2 . |
| first_indexed | 2024-03-08T16:29:17Z |
| format | Article |
| id | doaj.art-642fc3b4d33f470eb814e36066fe0e6a |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:29:17Z |
| publishDate | 2017-06-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-642fc3b4d33f470eb814e36066fe0e6a2024-01-06T10:17:19ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782017-06-018110.36326/kjvs/2017/v8i14302Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique.Najm Hadi NajimAmer Jebur Obayes AL-IsawiColonies of E.Coli O157:H7 were isolated from 80 locally produced Cow's and Buffalo's soft cheese samples that were collected randomly at weekly intervals (5 samples/ week) from various retail markets in different locations of babylon province during two climatic periods(40 samples /species/season) where the first periad was extended from the begining of December 2015 to the end of February 2016 while the second period was extended from the begining of July to the end of September 2016.The identification of E.Coli O157:H7 isolates were confirmed based on their cultural, biochemical and seralogical characteristics using the commercial latex agglutination test kit and by duplex PCR technique. Data revealed that there was a significant (P≤ 0.05) seasonal variation in the prevalence of E.Coli O157:H7 where all Cow's and Buffalo's soft cheese samples had significantly (P≤ 0.05) higher prevalence of E.Coli O157:H7 in summer season (50% and 40% respectively) than in winter season (25% and 15% respectively).It was found that all of the 80 bovine soft cheese samples had significantly (p<0.05) higher prevalence of E.Coli O157:H7 in summer (45%) than in winter (20%) seasons. The results of the diagnosis of E.coli O157:H7 were confirmed by PCR assay which showed that,six culturing isolates serotype E.coli O157:H7 were postive by PCR assay and five of these isolates expressed gene of Stx1 whearse one isolate expressed gene of Stx2 .https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4302Babylone. coliPCRStx1 and Stx2 genes |
| spellingShingle | Najm Hadi Najim Amer Jebur Obayes AL-Isawi Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique. Kufa Journal for Veterinary Medical Sciences Babylon e. coli PCR Stx1 and Stx2 genes |
| title | Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique. |
| title_full | Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique. |
| title_fullStr | Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique. |
| title_full_unstemmed | Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique. |
| title_short | Molecular detection of Stx1 and Stx2 genes in E.Coli O157:H7 isolated from Cow's and Buffalo's soft cheese samples inBabylon Province, using Multiplex PCR technique. |
| title_sort | molecular detection of stx1 and stx2 genes in e coli o157 h7 isolated from cow s and buffalo s soft cheese samples inbabylon province using multiplex pcr technique |
| topic | Babylon e. coli PCR Stx1 and Stx2 genes |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4302 |
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