Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers.
Campsis grandiflora (Bignoniaceae) is a fast growing deciduous climber, the dried flowers have been used as a carminative, blood tonic, and febrifuge in Chinese traditional medicine. This plant has an anti-inflammatory, anti-oxidant, anti-depressant, and anti-bacterial effect; with a beneficial role...
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| Format: | Article |
| Language: | English |
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College of Pharmacy University of Baghdad
2022-06-01
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| Series: | Iraqi Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1499 |
| _version_ | 1811245834306584576 |
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| author | Sara Saad Hassoon Ibrahim Saleh Abbas Bahir Abdul Razzaq Mshimesh |
| author_facet | Sara Saad Hassoon Ibrahim Saleh Abbas Bahir Abdul Razzaq Mshimesh |
| author_sort | Sara Saad Hassoon |
| collection | DOAJ |
| description | Campsis grandiflora (Bignoniaceae) is a fast growing deciduous climber, the dried flowers have been used as a carminative, blood tonic, and febrifuge in Chinese traditional medicine. This plant has an anti-inflammatory, anti-oxidant, anti-depressant, and anti-bacterial effect; with a beneficial role in stagnant blood and endometriosis conditions. In this study, the detection of beta-sitosterol in the hexane extract of Iraqi C.grandiflora flowers was performed using thin layer chromatography (TLC) and high performance liquid chromatography(HPLC); while the isolation done by preparative layer chromatography then structure elucidation of isolated compound was done by FTIR and 1HNMR. Furthermore, assessment of the anti-oxidant activity of the ethyl acetate extract of Iraqi C.grandiflora flowers using three different methods and total flavonoid content, then measuring the pearson’s correlation coefficient between these methods. The results showed that the hexane extract of Iraqi C.grandiflora flowers contain beta-sitosterol compound and the ethyl acetate extract of this plant possesses an excellent anti-oxidant effect using the single-electron transfer (SET) pathway in scavenging the free radicals, and this activity attributed to the potent antioxidant i.e. polyphenols. |
| first_indexed | 2024-04-12T14:45:14Z |
| format | Article |
| id | doaj.art-6431184f613c472ab5d29efdf6f421e9 |
| institution | Directory Open Access Journal |
| issn | 2521-3512 1683-3597 |
| language | English |
| last_indexed | 2024-04-12T14:45:14Z |
| publishDate | 2022-06-01 |
| publisher | College of Pharmacy University of Baghdad |
| record_format | Article |
| series | Iraqi Journal of Pharmaceutical Sciences |
| spelling | doaj.art-6431184f613c472ab5d29efdf6f421e92022-12-22T03:28:41ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences2521-35121683-35972022-06-0131117618310.31351/vol31iss1pp176-1831015Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers.Sara Saad Hassoon0Ibrahim Saleh AbbasBahir Abdul Razzaq Mshimeshbaghdad, iraqCampsis grandiflora (Bignoniaceae) is a fast growing deciduous climber, the dried flowers have been used as a carminative, blood tonic, and febrifuge in Chinese traditional medicine. This plant has an anti-inflammatory, anti-oxidant, anti-depressant, and anti-bacterial effect; with a beneficial role in stagnant blood and endometriosis conditions. In this study, the detection of beta-sitosterol in the hexane extract of Iraqi C.grandiflora flowers was performed using thin layer chromatography (TLC) and high performance liquid chromatography(HPLC); while the isolation done by preparative layer chromatography then structure elucidation of isolated compound was done by FTIR and 1HNMR. Furthermore, assessment of the anti-oxidant activity of the ethyl acetate extract of Iraqi C.grandiflora flowers using three different methods and total flavonoid content, then measuring the pearson’s correlation coefficient between these methods. The results showed that the hexane extract of Iraqi C.grandiflora flowers contain beta-sitosterol compound and the ethyl acetate extract of this plant possesses an excellent anti-oxidant effect using the single-electron transfer (SET) pathway in scavenging the free radicals, and this activity attributed to the potent antioxidant i.e. polyphenols.https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1499anti-oxidant activity, betasitosterol, campsis grandiflora, total flavonoids content. |
| spellingShingle | Sara Saad Hassoon Ibrahim Saleh Abbas Bahir Abdul Razzaq Mshimesh Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers. Iraqi Journal of Pharmaceutical Sciences anti-oxidant activity, betasitosterol, campsis grandiflora, total flavonoids content. |
| title | Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers. |
| title_full | Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers. |
| title_fullStr | Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers. |
| title_full_unstemmed | Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers. |
| title_short | Isolation of beta-sitosterol and evaluation of antioxidant Activity of Iraqi Campsis grandiflora flowers. |
| title_sort | isolation of beta sitosterol and evaluation of antioxidant activity of iraqi campsis grandiflora flowers |
| topic | anti-oxidant activity, betasitosterol, campsis grandiflora, total flavonoids content. |
| url | https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/1499 |
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