Ve-ptp modulates vascular integrity by promoting adherens junction maturation.
BACKGROUND: Endothelial cell junctions control blood vessel permeability. Altered permeability can be associated with vascular fragility that leads to vessel weakness and haemorrhage formation. In vivo studies on the function of genes involved in the maintenance of vascular integrity are essential t...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3522677?pdf=render |
| _version_ | 1818560563702136832 |
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| author | Silvia Carra Efrem Foglia Solei Cermenati Erica Bresciani Costanza Giampietro Carla Lora Lamia Elisabetta Dejana Monica Beltrame Franco Cotelli |
| author_facet | Silvia Carra Efrem Foglia Solei Cermenati Erica Bresciani Costanza Giampietro Carla Lora Lamia Elisabetta Dejana Monica Beltrame Franco Cotelli |
| author_sort | Silvia Carra |
| collection | DOAJ |
| description | BACKGROUND: Endothelial cell junctions control blood vessel permeability. Altered permeability can be associated with vascular fragility that leads to vessel weakness and haemorrhage formation. In vivo studies on the function of genes involved in the maintenance of vascular integrity are essential to better understand the molecular basis of diseases linked to permeability defects. Ve-ptp (Vascular Endothelial-Protein Tyrosine Phosphatase) is a transmembrane protein present at endothelial adherens junctions (AJs). METHODOLOGY/PRINCIPAL FINDINGS: We investigated the role of Ve-ptp in AJ maturation/stability and in the modulation of endothelial permeability using zebrafish (Danio rerio). Whole-mount in situ hybridizations revealed zve-ptp expression exclusively in the developing vascular system. Generation of altered zve-ptp transcripts, induced separately by two different splicing morpholinos, resulted in permeability defects closely linked to vascular wall fragility. The ultrastructural analysis revealed a statistically significant reduction of junction complexes and the presence of immature AJs in zve-ptp morphants but not in control embryos. CONCLUSIONS/SIGNIFICANCE: Here we show the first in vivo evidence of a potentially critical role played by Ve-ptp in AJ maturation, an important event for permeability modulation and for the development of a functional vascular system. |
| first_indexed | 2024-12-14T00:39:54Z |
| format | Article |
| id | doaj.art-6431f449ff574ef6a73227714e31aac1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T00:39:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6431f449ff574ef6a73227714e31aac12022-12-21T23:24:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5124510.1371/journal.pone.0051245Ve-ptp modulates vascular integrity by promoting adherens junction maturation.Silvia CarraEfrem FogliaSolei CermenatiErica BrescianiCostanza GiampietroCarla Lora LamiaElisabetta DejanaMonica BeltrameFranco CotelliBACKGROUND: Endothelial cell junctions control blood vessel permeability. Altered permeability can be associated with vascular fragility that leads to vessel weakness and haemorrhage formation. In vivo studies on the function of genes involved in the maintenance of vascular integrity are essential to better understand the molecular basis of diseases linked to permeability defects. Ve-ptp (Vascular Endothelial-Protein Tyrosine Phosphatase) is a transmembrane protein present at endothelial adherens junctions (AJs). METHODOLOGY/PRINCIPAL FINDINGS: We investigated the role of Ve-ptp in AJ maturation/stability and in the modulation of endothelial permeability using zebrafish (Danio rerio). Whole-mount in situ hybridizations revealed zve-ptp expression exclusively in the developing vascular system. Generation of altered zve-ptp transcripts, induced separately by two different splicing morpholinos, resulted in permeability defects closely linked to vascular wall fragility. The ultrastructural analysis revealed a statistically significant reduction of junction complexes and the presence of immature AJs in zve-ptp morphants but not in control embryos. CONCLUSIONS/SIGNIFICANCE: Here we show the first in vivo evidence of a potentially critical role played by Ve-ptp in AJ maturation, an important event for permeability modulation and for the development of a functional vascular system.http://europepmc.org/articles/PMC3522677?pdf=render |
| spellingShingle | Silvia Carra Efrem Foglia Solei Cermenati Erica Bresciani Costanza Giampietro Carla Lora Lamia Elisabetta Dejana Monica Beltrame Franco Cotelli Ve-ptp modulates vascular integrity by promoting adherens junction maturation. PLoS ONE |
| title | Ve-ptp modulates vascular integrity by promoting adherens junction maturation. |
| title_full | Ve-ptp modulates vascular integrity by promoting adherens junction maturation. |
| title_fullStr | Ve-ptp modulates vascular integrity by promoting adherens junction maturation. |
| title_full_unstemmed | Ve-ptp modulates vascular integrity by promoting adherens junction maturation. |
| title_short | Ve-ptp modulates vascular integrity by promoting adherens junction maturation. |
| title_sort | ve ptp modulates vascular integrity by promoting adherens junction maturation |
| url | http://europepmc.org/articles/PMC3522677?pdf=render |
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