Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing Factors
This study investigated ammonia-oxidizing bacterial communities in water and surface sediments of three tilapia ponds and their relationship with differences in the ponds, monthly variations in the water, and the physico-chemical parameters. Samples were collected from ponds with different stocking...
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MDPI AG
2022-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/7/3438 |
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| author | Limin Fan Liping Qiu Gengdong Hu Chao Song Shunlong Meng Dandan Li Jiazhang Chen |
| author_facet | Limin Fan Liping Qiu Gengdong Hu Chao Song Shunlong Meng Dandan Li Jiazhang Chen |
| author_sort | Limin Fan |
| collection | DOAJ |
| description | This study investigated ammonia-oxidizing bacterial communities in water and surface sediments of three tilapia ponds and their relationship with differences in the ponds, monthly variations in the water, and the physico-chemical parameters. Samples were collected from ponds with different stocking densities, after which DNA was extracted, 16S rRNA genes were amplified, the Illumina high-throughput sequencing was performed, and then the Silva and FunGene databases were used to investigate the ammonia-oxidizing bacterial communities. In total, 308,488 valid reads (144,931 in water and 163,517 in sediment) and 240 operational taxonomic units (207 in water and 225 in sediment) were obtained. Further analysis showed that the five genera of <i>Nitrosospira</i>, <i>Nitrosococcus</i>, <i>Nitrosomonas</i>, <i>Proteobacteria_unclassified</i>, and <i>Nitrosomonadaceae_unclassified</i> were distributed not only in the water, but also in surface sediments of all three ponds. Further, not only the abundance of these five genera, but also their diversities were affected by monthly variations in the water and by sediment differences among the ponds. Moreover, the total nitrogen (TN), nitrate, total phosphorus (TP), and sulphate were the main factors influencing the ammonia-oxidizing bacterial communities in the water, whereas TP was the main influencing factor in the sediments. Moreover, the parameter changes, especially those caused by differences in the ponds, were closely related to the cultivation management (stocking density and feed coefficients). |
| first_indexed | 2024-03-09T12:07:38Z |
| format | Article |
| id | doaj.art-643a914a572242e8914b5f5e7bc659e7 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T12:07:38Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-643a914a572242e8914b5f5e7bc659e72023-11-30T22:55:44ZengMDPI AGApplied Sciences2076-34172022-03-01127343810.3390/app12073438Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing FactorsLimin Fan0Liping Qiu1Gengdong Hu2Chao Song3Shunlong Meng4Dandan Li5Jiazhang Chen6Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi 214081, ChinaFreshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Wuxi 214081, ChinaThis study investigated ammonia-oxidizing bacterial communities in water and surface sediments of three tilapia ponds and their relationship with differences in the ponds, monthly variations in the water, and the physico-chemical parameters. Samples were collected from ponds with different stocking densities, after which DNA was extracted, 16S rRNA genes were amplified, the Illumina high-throughput sequencing was performed, and then the Silva and FunGene databases were used to investigate the ammonia-oxidizing bacterial communities. In total, 308,488 valid reads (144,931 in water and 163,517 in sediment) and 240 operational taxonomic units (207 in water and 225 in sediment) were obtained. Further analysis showed that the five genera of <i>Nitrosospira</i>, <i>Nitrosococcus</i>, <i>Nitrosomonas</i>, <i>Proteobacteria_unclassified</i>, and <i>Nitrosomonadaceae_unclassified</i> were distributed not only in the water, but also in surface sediments of all three ponds. Further, not only the abundance of these five genera, but also their diversities were affected by monthly variations in the water and by sediment differences among the ponds. Moreover, the total nitrogen (TN), nitrate, total phosphorus (TP), and sulphate were the main factors influencing the ammonia-oxidizing bacterial communities in the water, whereas TP was the main influencing factor in the sediments. Moreover, the parameter changes, especially those caused by differences in the ponds, were closely related to the cultivation management (stocking density and feed coefficients).https://www.mdpi.com/2076-3417/12/7/3438tilapia pondammonia-oxidizing bacterial communitiesstocking densityfeed coefficient |
| spellingShingle | Limin Fan Liping Qiu Gengdong Hu Chao Song Shunlong Meng Dandan Li Jiazhang Chen Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing Factors Applied Sciences tilapia pond ammonia-oxidizing bacterial communities stocking density feed coefficient |
| title | Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing Factors |
| title_full | Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing Factors |
| title_fullStr | Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing Factors |
| title_full_unstemmed | Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing Factors |
| title_short | Ammonia-Oxidizing Bacterial Communities in Tilapia Pond Systems and the Influencing Factors |
| title_sort | ammonia oxidizing bacterial communities in tilapia pond systems and the influencing factors |
| topic | tilapia pond ammonia-oxidizing bacterial communities stocking density feed coefficient |
| url | https://www.mdpi.com/2076-3417/12/7/3438 |
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