Retinal necrosis and apoptosis changes in mice under simulated microgravity
AIM: To investigate whether the microgravity environment is related to retinal damage.METHODS: Hanging-tail mice tests were used to simulate weightlessness. Light microscopy, and transmission electron microscopic examinations of the retinal tissue structure were used to observe morphological changes...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2022-11-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2022/11/202211001.pdf |
| _version_ | 1811246946530099200 |
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| author | Zhen-Fei Yang Wen-Jiong Li Peng Zhang Xu Zha Xiao Li Xiao-Ping Chen Si-Quan Zhu |
| author_facet | Zhen-Fei Yang Wen-Jiong Li Peng Zhang Xu Zha Xiao Li Xiao-Ping Chen Si-Quan Zhu |
| author_sort | Zhen-Fei Yang |
| collection | DOAJ |
| description | AIM: To investigate whether the microgravity environment is related to retinal damage.METHODS: Hanging-tail mice tests were used to simulate weightlessness. Light microscopy, and transmission electron microscopic examinations of the retinal tissue structure were used to observe morphological changes. Immunohistochemistry and Western blot analyses were performed to detect the molecular changes associated with the observations.RESULT: Light microscopy and transmission electron microscopy demonstrated that more dead cells were detected in the ganglion layer and inner nuclear layer cells. TNF-α and caspase-3 protein expression in the retinas of simulated microgravity groups were up-regulated compared with the ground-based control group.CONCLUSION: The results demonstrated that simulated microgravity produced severe pathological damage in the retinas of mice. |
| first_indexed | 2024-04-12T15:00:55Z |
| format | Article |
| id | doaj.art-643b63041c744a25bbc2656861b1077b |
| institution | Directory Open Access Journal |
| issn | 1672-5123 |
| language | English |
| last_indexed | 2024-04-12T15:00:55Z |
| publishDate | 2022-11-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-643b63041c744a25bbc2656861b1077b2022-12-22T03:28:04ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51232022-11-0122111765177010.3980/j.issn.1672-5123.2022.11.01202211001Retinal necrosis and apoptosis changes in mice under simulated microgravityZhen-Fei Yang0Wen-Jiong Li1Peng Zhang2Xu Zha3Xiao Li4Xiao-Ping Chen5Si-Quan Zhu6Department of Ophthalmology, Rehabilitation Hospital, National Research Center for Rehabilitation Technical Aids, Beijing 100176, ChinaState Key Laboratory of Space Medicine Fundamentals and Application, Astronaut Research and Training Center of China, Beijing 100094, ChinaState Key Laboratory of Space Medicine Fundamentals and Application, Astronaut Research and Training Center of China, Beijing 100094, ChinaDepartment of Ophthalmology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101,Yunnan Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, ChinaState Key Laboratory of Space Medicine Fundamentals and Application, Astronaut Research and Training Center of China, Beijing 100094, ChinaDepartment of Ophthalmology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, ChinaAIM: To investigate whether the microgravity environment is related to retinal damage.METHODS: Hanging-tail mice tests were used to simulate weightlessness. Light microscopy, and transmission electron microscopic examinations of the retinal tissue structure were used to observe morphological changes. Immunohistochemistry and Western blot analyses were performed to detect the molecular changes associated with the observations.RESULT: Light microscopy and transmission electron microscopy demonstrated that more dead cells were detected in the ganglion layer and inner nuclear layer cells. TNF-α and caspase-3 protein expression in the retinas of simulated microgravity groups were up-regulated compared with the ground-based control group.CONCLUSION: The results demonstrated that simulated microgravity produced severe pathological damage in the retinas of mice.http://ies.ijo.cn/cn_publish/2022/11/202211001.pdfsimulated microgravityretinatnf-αcaspase-3mice |
| spellingShingle | Zhen-Fei Yang Wen-Jiong Li Peng Zhang Xu Zha Xiao Li Xiao-Ping Chen Si-Quan Zhu Retinal necrosis and apoptosis changes in mice under simulated microgravity Guoji Yanke Zazhi simulated microgravity retina tnf-α caspase-3 mice |
| title | Retinal necrosis and apoptosis changes in mice under simulated microgravity |
| title_full | Retinal necrosis and apoptosis changes in mice under simulated microgravity |
| title_fullStr | Retinal necrosis and apoptosis changes in mice under simulated microgravity |
| title_full_unstemmed | Retinal necrosis and apoptosis changes in mice under simulated microgravity |
| title_short | Retinal necrosis and apoptosis changes in mice under simulated microgravity |
| title_sort | retinal necrosis and apoptosis changes in mice under simulated microgravity |
| topic | simulated microgravity retina tnf-α caspase-3 mice |
| url | http://ies.ijo.cn/cn_publish/2022/11/202211001.pdf |
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