Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass Spectrometry
Gastric helicobacters (<i>Helicobacter</i> (<i>H.</i>) <i>pylori</i> and non-<i>H. pylori Helicobacter</i> species (NHPHs)) colonize the stomach of humans and/or animals. <i>Helicobacter</i> species identification is essential since many of...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/3/366 |
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| author | Helena Berlamont Chloë De Witte Sofie De Bruyckere James G. Fox Steffen Backert Annemieke Smet Filip Boyen Freddy Haesebrouck |
| author_facet | Helena Berlamont Chloë De Witte Sofie De Bruyckere James G. Fox Steffen Backert Annemieke Smet Filip Boyen Freddy Haesebrouck |
| author_sort | Helena Berlamont |
| collection | DOAJ |
| description | Gastric helicobacters (<i>Helicobacter</i> (<i>H.</i>) <i>pylori</i> and non-<i>H. pylori Helicobacter</i> species (NHPHs)) colonize the stomach of humans and/or animals. <i>Helicobacter</i> species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, <i>Helicobacter</i> species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric <i>Helicobacter</i> isolates of 10 different <i>Helicobacter</i> species using MALDI-TOF MS in order to establish a more elaborate <i>Helicobacter</i> reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores ≥2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related <i>Helicobacter</i> species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric <i>Helicobacter</i> species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account. |
| first_indexed | 2024-03-10T13:06:46Z |
| format | Article |
| id | doaj.art-643d2780b2504adeb88b823c29e99d18 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T13:06:46Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-643d2780b2504adeb88b823c29e99d182023-11-21T11:04:15ZengMDPI AGPathogens2076-08172021-03-0110336610.3390/pathogens10030366Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass SpectrometryHelena Berlamont0Chloë De Witte1Sofie De Bruyckere2James G. Fox3Steffen Backert4Annemieke Smet5Filip Boyen6Freddy Haesebrouck7Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USADepartment Biology, Division Microbiology, University Erlangen Nuremberg, 91058 Erlangen, GermanyLaboratory of Experimental Medicine and Pediatrics, Faculty of Medicine and Health Sciences, University of Antwerp, 2610 Antwerp, BelgiumDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumDepartment of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, BelgiumGastric helicobacters (<i>Helicobacter</i> (<i>H.</i>) <i>pylori</i> and non-<i>H. pylori Helicobacter</i> species (NHPHs)) colonize the stomach of humans and/or animals. <i>Helicobacter</i> species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, <i>Helicobacter</i> species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric <i>Helicobacter</i> isolates of 10 different <i>Helicobacter</i> species using MALDI-TOF MS in order to establish a more elaborate <i>Helicobacter</i> reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores ≥2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related <i>Helicobacter</i> species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric <i>Helicobacter</i> species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account.https://www.mdpi.com/2076-0817/10/3/366gastric <i>Helicobacter</i> speciesNHPHMALDI-TOF MS |
| spellingShingle | Helena Berlamont Chloë De Witte Sofie De Bruyckere James G. Fox Steffen Backert Annemieke Smet Filip Boyen Freddy Haesebrouck Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass Spectrometry Pathogens gastric <i>Helicobacter</i> species NHPH MALDI-TOF MS |
| title | Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass Spectrometry |
| title_full | Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass Spectrometry |
| title_fullStr | Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass Spectrometry |
| title_full_unstemmed | Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass Spectrometry |
| title_short | Differentiation of Gastric <i>Helicobacter</i> Species Using MALDI-TOF Mass Spectrometry |
| title_sort | differentiation of gastric i helicobacter i species using maldi tof mass spectrometry |
| topic | gastric <i>Helicobacter</i> species NHPH MALDI-TOF MS |
| url | https://www.mdpi.com/2076-0817/10/3/366 |
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