Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil EC
Crocus sativus is a triploid sterile plant with red stigmas belonging to family of Iridaceae, and sub family Crocoideae. Crocin, picrocrocin, and safranal are three major carotenoid derivatives that are responsible for color, taste and specific aroma of Crocus. Saffron flowers are harvested manuall...
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| Format: | Article |
| Language: | English |
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Politeknik Negeri Jember
2023-03-01
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| Series: | Agriprima: Journal of Applied Agricultural Sciences |
| Subjects: | |
| Online Access: | https://agriprima.polije.ac.id/index.php/journal/article/view/508 |
| _version_ | 1797854669384450048 |
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| author | Mandana Mirbakhsh Zahra Zahed Sepideh Mashayekhi Monire Jafari |
| author_facet | Mandana Mirbakhsh Zahra Zahed Sepideh Mashayekhi Monire Jafari |
| author_sort | Mandana Mirbakhsh |
| collection | DOAJ |
| description |
Crocus sativus is a triploid sterile plant with red stigmas belonging to family of Iridaceae, and sub family Crocoideae. Crocin, picrocrocin, and safranal are three major carotenoid derivatives that are responsible for color, taste and specific aroma of Crocus. Saffron flowers are harvested manually and used as spice, dye or medicinal applications. The natural propagation rate of most geophytes including saffron is relatively low. An in vitro multiplication technique like micropropagation has been used for the propagation of saffron. To understand the efficiency of this alternative and study the molecular basis of apocarotenoid biosynthesis/accumulation, the RT-PCR method was performed on perianth explants that were cultured on MS medium to observe the level of expression of zeaxanthin cleavage dioxygenase (CsZCD) gene during stigma development, and also the impact of soil EC on its expression. The present study was conducted at Plant molecular and physiology Lab, Alzahra University, Tehran, Iran during 2011-2013. Stigma-like structures (SLSs) on calli were collected from immature perianth explants from floral buds of corms that were collected from Ghaen city, and compared to (Torbat-e Haidariye, Mardabad and Shahroud cities) for investigating the impact of different soil EC on CsZCD expression. The results indicated that CsZCD gene was highly expressed in fully developed red SLSs in perianth of cultured samples of Shahroud with the highest salinity. In this research, a close relationship between soil EC and second metabolites regulation is studied. Overall, these results will pave the way for understanding the molecular basis of apocarotenoid biosynthesis and other aspects of stigma development in C. sativus.
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| first_indexed | 2024-04-09T20:10:55Z |
| format | Article |
| id | doaj.art-643ea1311f2f414cac686ffa66f2d5d0 |
| institution | Directory Open Access Journal |
| issn | 2549-2934 2549-2942 |
| language | English |
| last_indexed | 2024-04-09T20:10:55Z |
| publishDate | 2023-03-01 |
| publisher | Politeknik Negeri Jember |
| record_format | Article |
| series | Agriprima: Journal of Applied Agricultural Sciences |
| spelling | doaj.art-643ea1311f2f414cac686ffa66f2d5d02023-03-31T23:33:59ZengPoliteknik Negeri JemberAgriprima: Journal of Applied Agricultural Sciences2549-29342549-29422023-03-017110.25047/agriprima.v7i1.508Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil ECMandana Mirbakhsh0Zahra Zahed1Sepideh Mashayekhi2Monire Jafari3Purdue University West LafayetteAlzahra UniversityAlzahra UniversityTexas State University Crocus sativus is a triploid sterile plant with red stigmas belonging to family of Iridaceae, and sub family Crocoideae. Crocin, picrocrocin, and safranal are three major carotenoid derivatives that are responsible for color, taste and specific aroma of Crocus. Saffron flowers are harvested manually and used as spice, dye or medicinal applications. The natural propagation rate of most geophytes including saffron is relatively low. An in vitro multiplication technique like micropropagation has been used for the propagation of saffron. To understand the efficiency of this alternative and study the molecular basis of apocarotenoid biosynthesis/accumulation, the RT-PCR method was performed on perianth explants that were cultured on MS medium to observe the level of expression of zeaxanthin cleavage dioxygenase (CsZCD) gene during stigma development, and also the impact of soil EC on its expression. The present study was conducted at Plant molecular and physiology Lab, Alzahra University, Tehran, Iran during 2011-2013. Stigma-like structures (SLSs) on calli were collected from immature perianth explants from floral buds of corms that were collected from Ghaen city, and compared to (Torbat-e Haidariye, Mardabad and Shahroud cities) for investigating the impact of different soil EC on CsZCD expression. The results indicated that CsZCD gene was highly expressed in fully developed red SLSs in perianth of cultured samples of Shahroud with the highest salinity. In this research, a close relationship between soil EC and second metabolites regulation is studied. Overall, these results will pave the way for understanding the molecular basis of apocarotenoid biosynthesis and other aspects of stigma development in C. sativus. https://agriprima.polije.ac.id/index.php/journal/article/view/508Crocus sativusmicropropagationgene expressionsalinity stress |
| spellingShingle | Mandana Mirbakhsh Zahra Zahed Sepideh Mashayekhi Monire Jafari Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil EC Agriprima: Journal of Applied Agricultural Sciences Crocus sativus micropropagation gene expression salinity stress |
| title | Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil EC |
| title_full | Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil EC |
| title_fullStr | Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil EC |
| title_full_unstemmed | Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil EC |
| title_short | Investigation of In Vitro Apocarotenoid Expression in Perianth of Saffron (Crocus sativus L.) Under Different Soil EC |
| title_sort | investigation of in vitro apocarotenoid expression in perianth of saffron crocus sativus l under different soil ec |
| topic | Crocus sativus micropropagation gene expression salinity stress |
| url | https://agriprima.polije.ac.id/index.php/journal/article/view/508 |
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