Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implications
ABSTRACT This study aimed to identify the Staphylococcus species responsible for bovine mastitis in dairy herds in northern Brazil, to investigate the capacity of biofilm production, and to analyze the association of biofilm production with multiresistance and intensity of California Mastitis Tests...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Minas Gerais
2022-08-01
|
| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400563&tlng=en |
| _version_ | 1818516570132971520 |
|---|---|
| author | L.F.S. Israel R.F. Rabello L.B. Ferreira R.C. Peruquetti L.S. Medeiros |
| author_facet | L.F.S. Israel R.F. Rabello L.B. Ferreira R.C. Peruquetti L.S. Medeiros |
| author_sort | L.F.S. Israel |
| collection | DOAJ |
| description | ABSTRACT This study aimed to identify the Staphylococcus species responsible for bovine mastitis in dairy herds in northern Brazil, to investigate the capacity of biofilm production, and to analyze the association of biofilm production with multiresistance and intensity of California Mastitis Tests (CMT) reactions that can make treatment more difficult and cause misdiagnoses, respectively. Milk samples were collected from 23 dairy farms located in five municipalities in the state of Acre. A total of 339 crossbred cows were tested by CMT, with 109 animals (229 udder ceilings) reacting to the test. After bacterial isolation in blood agar, the catalase-positive and gram-positive cocci were submitted for identification by MALDI-TOF MS. Of 103 strains identified as staphylococci, Staphylococcus chromogenes (58.3%) and Staphylococcus aureus (19.4%) were the most prevalent species. Biofilm production was quantitatively evaluated using a microplate adherence test. Among the Staphylococcus strains, 71.8% were biofilm producers. Most strains of S. chromogenes (68.3%) had the capacity to produce biofilms, ranging from weak (43.3%), moderate (13.3%), and strong (11.7%) producers. Among S. aureus strains, 50% were non-biofilm producers, and none were strong producers. Our data showed an association between biofilm production capacity and multidrug resistance. In addition, there was a reduction in the response to the CMT test, which can mask the diagnosis. |
| first_indexed | 2024-12-11T00:44:05Z |
| format | Article |
| id | doaj.art-64425a7be0b54865891775bcdb027735 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-11T00:44:05Z |
| publishDate | 2022-08-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-64425a7be0b54865891775bcdb0277352022-12-22T01:26:50ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622022-08-0174456357510.1590/1678-4162-12271Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implicationsL.F.S. Israelhttps://orcid.org/0000-0003-3057-4219R.F. Rabellohttps://orcid.org/0000-0002-4895-4538L.B. Ferreirahttps://orcid.org/0000-0002-3509-1745R.C. Peruquettihttps://orcid.org/0000-0002-0807-208XL.S. Medeiroshttps://orcid.org/0000-0001-5145-0915ABSTRACT This study aimed to identify the Staphylococcus species responsible for bovine mastitis in dairy herds in northern Brazil, to investigate the capacity of biofilm production, and to analyze the association of biofilm production with multiresistance and intensity of California Mastitis Tests (CMT) reactions that can make treatment more difficult and cause misdiagnoses, respectively. Milk samples were collected from 23 dairy farms located in five municipalities in the state of Acre. A total of 339 crossbred cows were tested by CMT, with 109 animals (229 udder ceilings) reacting to the test. After bacterial isolation in blood agar, the catalase-positive and gram-positive cocci were submitted for identification by MALDI-TOF MS. Of 103 strains identified as staphylococci, Staphylococcus chromogenes (58.3%) and Staphylococcus aureus (19.4%) were the most prevalent species. Biofilm production was quantitatively evaluated using a microplate adherence test. Among the Staphylococcus strains, 71.8% were biofilm producers. Most strains of S. chromogenes (68.3%) had the capacity to produce biofilms, ranging from weak (43.3%), moderate (13.3%), and strong (11.7%) producers. Among S. aureus strains, 50% were non-biofilm producers, and none were strong producers. Our data showed an association between biofilm production capacity and multidrug resistance. In addition, there was a reduction in the response to the CMT test, which can mask the diagnosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400563&tlng=enbiofilmbovine mastitisStaphylococcus chromogenesStaphylococcus aureusmultidrug resistance |
| spellingShingle | L.F.S. Israel R.F. Rabello L.B. Ferreira R.C. Peruquetti L.S. Medeiros Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implications Arquivo Brasileiro de Medicina Veterinária e Zootecnia biofilm bovine mastitis Staphylococcus chromogenes Staphylococcus aureus multidrug resistance |
| title | Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implications |
| title_full | Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implications |
| title_fullStr | Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implications |
| title_full_unstemmed | Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implications |
| title_short | Biofilm production by Staphylococcus spp. isolated from bovine mastitis in dairy herds in state of Acre, Brazil and its implications |
| title_sort | biofilm production by staphylococcus spp isolated from bovine mastitis in dairy herds in state of acre brazil and its implications |
| topic | biofilm bovine mastitis Staphylococcus chromogenes Staphylococcus aureus multidrug resistance |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400563&tlng=en |
| work_keys_str_mv | AT lfsisrael biofilmproductionbystaphylococcussppisolatedfrombovinemastitisindairyherdsinstateofacrebrazilanditsimplications AT rfrabello biofilmproductionbystaphylococcussppisolatedfrombovinemastitisindairyherdsinstateofacrebrazilanditsimplications AT lbferreira biofilmproductionbystaphylococcussppisolatedfrombovinemastitisindairyherdsinstateofacrebrazilanditsimplications AT rcperuquetti biofilmproductionbystaphylococcussppisolatedfrombovinemastitisindairyherdsinstateofacrebrazilanditsimplications AT lsmedeiros biofilmproductionbystaphylococcussppisolatedfrombovinemastitisindairyherdsinstateofacrebrazilanditsimplications |