Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations
Abstract Background Plasmodiophora brassicae is a soilborne pathogen of the family Brassicaceae and the causal agent of clubroot disease. In Canada, P. brassicae is now one of the most important constraints to canola (Brassica napus) production, and is managed mainly by the deployment of resistant c...
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4658-1 |
| _version_ | 1818272106000941056 |
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| author | Michael D. Holtz Sheau-Fang Hwang Stephen E. Strelkov |
| author_facet | Michael D. Holtz Sheau-Fang Hwang Stephen E. Strelkov |
| author_sort | Michael D. Holtz |
| collection | DOAJ |
| description | Abstract Background Plasmodiophora brassicae is a soilborne pathogen of the family Brassicaceae and the causal agent of clubroot disease. In Canada, P. brassicae is now one of the most important constraints to canola (Brassica napus) production, and is managed mainly by the deployment of resistant cultivars. In recent years, however, new strains of the pathogen have emerged that are capable of overcoming host resistance, posing new challenges for disease management. Despite its economic significance, molecular studies of P. brassicae are rare, mainly because this microorganism cannot be cultured outside of its host. Results Restriction site-associated DNA sequencing (RADseq) was used to examine the genetic diversity within P. brassicae single-spore and field isolates collected from across Canada. The isolates included individuals that were either capable or incapable of causing disease on clubroot resistant canola cultivars. Over 8750 variants were identified through RADseq. Population analysis indicated that most isolates belonged to one of two distinct populations, corresponding with the ability of isolates to cause disease on resistant cultivars. Within each population, there were low levels of genetic diversity. One thousand and fifty of the genetic variants that distinguished the two populations were nonsynonymous, altering the coding sequences of genes. Conclusion The application of RADseq revealed two distinct populations of P. brassicae in Canada, suggesting multiple introductions of the pathogen into the country. The genetic variation found here will be important for future research and monitoring of the pathogen. |
| first_indexed | 2024-12-12T21:36:47Z |
| format | Article |
| id | doaj.art-64469710dbb8488d980d44c303e9d1f4 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-12T21:36:47Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-64469710dbb8488d980d44c303e9d1f42022-12-22T00:11:10ZengBMCBMC Genomics1471-21642018-04-0119111010.1186/s12864-018-4658-1Genotyping of Plasmodiophora brassicae reveals the presence of distinct populationsMichael D. Holtz0Sheau-Fang Hwang1Stephen E. Strelkov2Alberta Agriculture and Forestry, Field Crop Development CentreAlberta Agriculture and Forestry, Crop Diversification Centre NorthDepartment of Agricultural, Food and Nutritional Science, University of AlbertaAbstract Background Plasmodiophora brassicae is a soilborne pathogen of the family Brassicaceae and the causal agent of clubroot disease. In Canada, P. brassicae is now one of the most important constraints to canola (Brassica napus) production, and is managed mainly by the deployment of resistant cultivars. In recent years, however, new strains of the pathogen have emerged that are capable of overcoming host resistance, posing new challenges for disease management. Despite its economic significance, molecular studies of P. brassicae are rare, mainly because this microorganism cannot be cultured outside of its host. Results Restriction site-associated DNA sequencing (RADseq) was used to examine the genetic diversity within P. brassicae single-spore and field isolates collected from across Canada. The isolates included individuals that were either capable or incapable of causing disease on clubroot resistant canola cultivars. Over 8750 variants were identified through RADseq. Population analysis indicated that most isolates belonged to one of two distinct populations, corresponding with the ability of isolates to cause disease on resistant cultivars. Within each population, there were low levels of genetic diversity. One thousand and fifty of the genetic variants that distinguished the two populations were nonsynonymous, altering the coding sequences of genes. Conclusion The application of RADseq revealed two distinct populations of P. brassicae in Canada, suggesting multiple introductions of the pathogen into the country. The genetic variation found here will be important for future research and monitoring of the pathogen.http://link.springer.com/article/10.1186/s12864-018-4658-1ClubrootPlasmodiophora brassicaePopulation structureRAD sequencing |
| spellingShingle | Michael D. Holtz Sheau-Fang Hwang Stephen E. Strelkov Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations BMC Genomics Clubroot Plasmodiophora brassicae Population structure RAD sequencing |
| title | Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations |
| title_full | Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations |
| title_fullStr | Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations |
| title_full_unstemmed | Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations |
| title_short | Genotyping of Plasmodiophora brassicae reveals the presence of distinct populations |
| title_sort | genotyping of plasmodiophora brassicae reveals the presence of distinct populations |
| topic | Clubroot Plasmodiophora brassicae Population structure RAD sequencing |
| url | http://link.springer.com/article/10.1186/s12864-018-4658-1 |
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