Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
Abstract DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a for...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-03-01
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| Series: | Global Challenges |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/gch2.201900075 |
| _version_ | 1818609015707402240 |
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| author | Xi Chen Falin Tian Min Li Haijiao Xu Mingjun Cai Qian Li Xiaolei Zuo Hongda Wang Xinghua Shi Chunhai Fan Huricha Baigude Yuping Shan |
| author_facet | Xi Chen Falin Tian Min Li Haijiao Xu Mingjun Cai Qian Li Xiaolei Zuo Hongda Wang Xinghua Shi Chunhai Fan Huricha Baigude Yuping Shan |
| author_sort | Xi Chen |
| collection | DOAJ |
| description | Abstract DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s−1. Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single‐molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin‐mediated endocytosis. |
| first_indexed | 2024-12-16T14:51:49Z |
| format | Article |
| id | doaj.art-6447d43b0d8249a28adc581bff44fb40 |
| institution | Directory Open Access Journal |
| issn | 2056-6646 |
| language | English |
| last_indexed | 2024-12-16T14:51:49Z |
| publishDate | 2020-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Global Challenges |
| spelling | doaj.art-6447d43b0d8249a28adc581bff44fb402022-12-21T22:27:34ZengWileyGlobal Challenges2056-66462020-03-0143n/an/a10.1002/gch2.201900075Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA NanostructuresXi Chen0Falin Tian1Min Li2Haijiao Xu3Mingjun Cai4Qian Li5Xiaolei Zuo6Hongda Wang7Xinghua Shi8Chunhai Fan9Huricha Baigude10Yuping Shan11School of Chemistry and Life Science Advanced Institute of Materials Science Changchun University of Technology Changchun Jilin 130012 ChinaLaboratory of Theoretical and Computational Nanoscience CAS Key Laboratory for Nanosystem and Hierarchy Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences Beijing 100190 ChinaSchool of Chemistry and Chemical Engineering and Institute of Molecular Medicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200240 ChinaState Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 ChinaState Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 ChinaSchool of Chemistry and Chemical Engineering and Institute of Molecular Medicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200240 ChinaSchool of Chemistry and Chemical Engineering and Institute of Molecular Medicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200240 ChinaState Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022 ChinaLaboratory of Theoretical and Computational Nanoscience CAS Key Laboratory for Nanosystem and Hierarchy Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Chinese Academy of Sciences Beijing 100190 ChinaSchool of Chemistry and Chemical Engineering and Institute of Molecular Medicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200240 ChinaSchool of Chemistry & Chemical Engineering Inner Mongolia Key Laboratory of Mongolian Medicinal Chemistry Inner Mongolia University Hohhot Inner Mongolia 010020 ChinaSchool of Chemistry and Life Science Advanced Institute of Materials Science Changchun University of Technology Changchun Jilin 130012 ChinaAbstract DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s−1. Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single‐molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin‐mediated endocytosis.https://doi.org/10.1002/gch2.201900075force tracingreal‐time trackingsingle tetrahedral DNA nanostructurestransmembrane kinetics |
| spellingShingle | Xi Chen Falin Tian Min Li Haijiao Xu Mingjun Cai Qian Li Xiaolei Zuo Hongda Wang Xinghua Shi Chunhai Fan Huricha Baigude Yuping Shan Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures Global Challenges force tracing real‐time tracking single tetrahedral DNA nanostructures transmembrane kinetics |
| title | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
| title_full | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
| title_fullStr | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
| title_full_unstemmed | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
| title_short | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
| title_sort | size independent transmembrane transporting of single tetrahedral dna nanostructures |
| topic | force tracing real‐time tracking single tetrahedral DNA nanostructures transmembrane kinetics |
| url | https://doi.org/10.1002/gch2.201900075 |
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