Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study
Embryo assessment methods changed during the last 20 years. Morphological criteria used for embryo quality assessment improved in order to increase the selection probability of the most suitable embryos for transfer. This paper is presenting, based on a rich practical experience cumulated in our res...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1786 |
| _version_ | 1827289343132172288 |
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| author | Alexandra Ivan Nicolae Păcală Ada Cean Valeriu Carabă |
| author_facet | Alexandra Ivan Nicolae Păcală Ada Cean Valeriu Carabă |
| author_sort | Alexandra Ivan |
| collection | DOAJ |
| description | Embryo assessment methods changed during the last 20 years. Morphological criteria used for embryo quality assessment improved in order to increase the selection probability of the most suitable embryos for transfer. This paper is presenting, based on a rich practical experience cumulated in our research group, several practical methods used for embryo assessment. For this study we have comparative evaluate the embryo quality using four staining tests: Trypan blue staining, Neutral red staining, Fluorescein diacetate staining, Propidium iodide staining in order to establish which test is the most reliable and efficient in embryo quality evaluation. The results obtained indicated that the Fluorescein diacetate and Propidium iodide viability test can be successfully used for mouse embryo viability evaluation. This method allows detection of both viable and nonviable cells. |
| first_indexed | 2024-04-24T11:49:04Z |
| format | Article |
| id | doaj.art-644a063e6ac449b89b581cf550eeca10 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:49:04Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-644a063e6ac449b89b581cf550eeca102024-04-09T08:38:11ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014414204201786Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative StudyAlexandra Ivan0Nicolae Păcală1Ada Cean2Valeriu Carabă3Banat University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara, Calea Aradului 119, RomaniaBanat University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara, Calea Aradului 119, RomaniaBanat University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara, Calea Aradului 119, RomaniaBanat University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara, Calea Aradului 119, RomaniaEmbryo assessment methods changed during the last 20 years. Morphological criteria used for embryo quality assessment improved in order to increase the selection probability of the most suitable embryos for transfer. This paper is presenting, based on a rich practical experience cumulated in our research group, several practical methods used for embryo assessment. For this study we have comparative evaluate the embryo quality using four staining tests: Trypan blue staining, Neutral red staining, Fluorescein diacetate staining, Propidium iodide staining in order to establish which test is the most reliable and efficient in embryo quality evaluation. The results obtained indicated that the Fluorescein diacetate and Propidium iodide viability test can be successfully used for mouse embryo viability evaluation. This method allows detection of both viable and nonviable cells.https://spasb.ro/index.php/public_html/article/view/1786mouse embryoqualitystaining tests |
| spellingShingle | Alexandra Ivan Nicolae Păcală Ada Cean Valeriu Carabă Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study Scientific Papers Animal Science and Biotechnologies mouse embryo quality staining tests |
| title | Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study |
| title_full | Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study |
| title_fullStr | Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study |
| title_full_unstemmed | Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study |
| title_short | Practical Methods to Assess Mammalian Embryo Quality – Staining Tests Comparative Study |
| title_sort | practical methods to assess mammalian embryo quality staining tests comparative study |
| topic | mouse embryo quality staining tests |
| url | https://spasb.ro/index.php/public_html/article/view/1786 |
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