Defining Proximity Proteome of Histone Modifications by Antibody-mediated Protein A-APEX2 Labeling

Defining Proximity Proteome of Histone Modifications by Antibody-mediated Protein A-APEX2 Labeling

Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approach to characterize multiprotein complexes and protein–protein interactions. However, current methods depend on the expression of exogenous fusion proteins and cannot be applied to identify proteins su...

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Bibliographic Details
Main Authors: Xinran Li, Jiaqi Zhou, Wenjuan Zhao, Qing Wen, Weijie Wang, Huipai Peng, Yuan Gao, Kelly J. Bouchonville, Steven M. Offer, Kuiming Chan, Zhiquan Wang, Nan Li, Haiyun Gan
Format: Article
Language:English
Published: Oxford University Press 2022-02-01
Series:Genomics, Proteomics & Bioinformatics
Subjects:
Proximity labeling
Post-translationally
AMAPEX
Modified histone
Online Access:http://www.sciencedirect.com/science/article/pii/S1672022921001820

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http://www.sciencedirect.com/science/article/pii/S1672022921001820

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