Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykiss
Flavobacterium psychrophilum is the causative agent of Bacterial Cold Water Disease (BCWD)/ Rainbow Trout Fry Syndrome (RTFS) in rainbow trout Oncorhynchus mykiss. The disease is associated with significant mortality in trout farms and thereby responsible for severe economical losses in this part of...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423001126 |
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| author | Heidi Mathiessen Yajiao Duan Moonika H. Marana Shaozhi Zuo Asma M. Karami Rzgar Jafaar Louise von Gersdorff Jørgensen Per W. Kania Inger Dalsgaard Lone Madsen Torben Nielsen Fabian Grammes Jørgen Ødegård Valeria Macchia Kurt Buchmann |
| author_facet | Heidi Mathiessen Yajiao Duan Moonika H. Marana Shaozhi Zuo Asma M. Karami Rzgar Jafaar Louise von Gersdorff Jørgensen Per W. Kania Inger Dalsgaard Lone Madsen Torben Nielsen Fabian Grammes Jørgen Ødegård Valeria Macchia Kurt Buchmann |
| author_sort | Heidi Mathiessen |
| collection | DOAJ |
| description | Flavobacterium psychrophilum is the causative agent of Bacterial Cold Water Disease (BCWD)/ Rainbow Trout Fry Syndrome (RTFS) in rainbow trout Oncorhynchus mykiss. The disease is associated with significant mortality in trout farms and thereby responsible for severe economical losses in this part of the aquaculture industry. Vaccination of the very young life cycle stages is not successful due to the immature development of the adaptive immunity in yolksac larvae and early fry. This explains the extensive usage of antibiotics for control of BCWD/RTFS. Selective breeding of RTFS resistant fish may be a solution, and identification of markers associated with natural susceptibility/resistance to the disease may elevate breeding efforts towards more robust strains. We suggest a QTL (SNP Affx-88941461) for partial disease resistance on chromosome 25 (Omy25) based on our experimental F. psychrophilum challenges of outbred fish and subsequent GWAS analyses. The favourable SNP allele was designated Q, whereas q indicated the unfavourable allele. We validated this QTL in two subsequent challenge experiments by F. psychrophilum exposure of QQ, Qq and qq trout. In the first trial we produced trout carrying at least one favourable allele (QQ and Qq) associated with partial resistance by fertilizing trout eggs from non-selected (outbred) females with sperm from homozygous QQ males. In the second trial we also produced homozygous offspring by fertilizing trout eggs from QQ females with sperm from QQ males. The resistance profiles of these groups were then determined in the laboratory by triplicate or duplicate challenge experiments (common garden water bath exposure to F. psychrophilum), which showed a significantly higher survival in trout carrying the favourable allele. Field observations supplemented the laboratory studies. Under farm conditions QQ and Qq trout showed a higher survival rate compared to qq trout. In the present study chromosome 25 (Omy25) QTL was found associated with a partial resistance to F. psychrophilum. However, we recommend to include additional SNPs in future breeding studies because several QTL are associated with resistance. |
| first_indexed | 2024-03-13T04:55:33Z |
| format | Article |
| id | doaj.art-644a80380f6544de9c1d172a76b2e1d1 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-13T04:55:33Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-644a80380f6544de9c1d172a76b2e1d12023-06-18T05:02:24ZengElsevierAquaculture Reports2352-51342023-06-0130101573Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykissHeidi Mathiessen0Yajiao Duan1Moonika H. Marana2Shaozhi Zuo3Asma M. Karami4Rzgar Jafaar5Louise von Gersdorff Jørgensen6Per W. Kania7Inger Dalsgaard8Lone Madsen9Torben Nielsen10Fabian Grammes11Jørgen Ødegård12Valeria Macchia13Kurt Buchmann14Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., DenmarkInstitute of Aquatic Resources, Technical University of Denmark, Kgs. DK-2800, Lyngby, DenmarkInstitute of Aquatic Resources, Technical University of Denmark, Kgs. DK-2800, Lyngby, DenmarkAquaSearch ova ApS, DK-7300 Jelling, DenmarkAquaGen AS, P.O. Box 1240, NO-7462 Trondheim, NorwayAquaGen AS, P.O. Box 1240, NO-7462 Trondheim, NorwayInstitute of Aquaculture, University of Stirling, FK9 4LA Scotland, UKDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-1870 Frederiksberg C., Denmark; Corresponding author.Flavobacterium psychrophilum is the causative agent of Bacterial Cold Water Disease (BCWD)/ Rainbow Trout Fry Syndrome (RTFS) in rainbow trout Oncorhynchus mykiss. The disease is associated with significant mortality in trout farms and thereby responsible for severe economical losses in this part of the aquaculture industry. Vaccination of the very young life cycle stages is not successful due to the immature development of the adaptive immunity in yolksac larvae and early fry. This explains the extensive usage of antibiotics for control of BCWD/RTFS. Selective breeding of RTFS resistant fish may be a solution, and identification of markers associated with natural susceptibility/resistance to the disease may elevate breeding efforts towards more robust strains. We suggest a QTL (SNP Affx-88941461) for partial disease resistance on chromosome 25 (Omy25) based on our experimental F. psychrophilum challenges of outbred fish and subsequent GWAS analyses. The favourable SNP allele was designated Q, whereas q indicated the unfavourable allele. We validated this QTL in two subsequent challenge experiments by F. psychrophilum exposure of QQ, Qq and qq trout. In the first trial we produced trout carrying at least one favourable allele (QQ and Qq) associated with partial resistance by fertilizing trout eggs from non-selected (outbred) females with sperm from homozygous QQ males. In the second trial we also produced homozygous offspring by fertilizing trout eggs from QQ females with sperm from QQ males. The resistance profiles of these groups were then determined in the laboratory by triplicate or duplicate challenge experiments (common garden water bath exposure to F. psychrophilum), which showed a significantly higher survival in trout carrying the favourable allele. Field observations supplemented the laboratory studies. Under farm conditions QQ and Qq trout showed a higher survival rate compared to qq trout. In the present study chromosome 25 (Omy25) QTL was found associated with a partial resistance to F. psychrophilum. However, we recommend to include additional SNPs in future breeding studies because several QTL are associated with resistance.http://www.sciencedirect.com/science/article/pii/S2352513423001126QTLRainbow troutRTFSBCWDBreeding |
| spellingShingle | Heidi Mathiessen Yajiao Duan Moonika H. Marana Shaozhi Zuo Asma M. Karami Rzgar Jafaar Louise von Gersdorff Jørgensen Per W. Kania Inger Dalsgaard Lone Madsen Torben Nielsen Fabian Grammes Jørgen Ødegård Valeria Macchia Kurt Buchmann Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykiss Aquaculture Reports QTL Rainbow trout RTFS BCWD Breeding |
| title | Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykiss |
| title_full | Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykiss |
| title_fullStr | Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykiss |
| title_full_unstemmed | Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykiss |
| title_short | Validation of a QTL for Flavobacterium psychrophilum resistance in rainbow trout Oncorhynchus mykiss |
| title_sort | validation of a qtl for flavobacterium psychrophilum resistance in rainbow trout oncorhynchus mykiss |
| topic | QTL Rainbow trout RTFS BCWD Breeding |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423001126 |
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