Two-hybrid screening of FAM13A protein partners in lung epithelial cells
Abstract Objectives Family with sequence similarity 13 member A (FAM13A) genetic variants have been associated with several chronic respiratory diseases including chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and lung cancer. The FAM13A prote...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-019-4840-9 |
| _version_ | 1819296423705837568 |
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| author | Manon Ruffin Kristin E. Thompson Harriet Corvol Loic Guillot |
| author_facet | Manon Ruffin Kristin E. Thompson Harriet Corvol Loic Guillot |
| author_sort | Manon Ruffin |
| collection | DOAJ |
| description | Abstract Objectives Family with sequence similarity 13 member A (FAM13A) genetic variants have been associated with several chronic respiratory diseases including chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and lung cancer. The FAM13A protein includes a RhoGTPase activating protein (RhoGAP) domain known to participate in various cellular mechanisms including cell proliferation. While intensive genomic studies have been performed to reveal its involvement in lung diseases, the biological role of FAM13A protein is still not completely elucidated. Results We therefore performed a two-hybrid screening to identify protein partners of FAM13A using a human lung cancer cDNA library. We identified several protein partners with a high confidence score. Researchers in the field of chronic lung diseases may benefit from this two-hybrid screening data which may reveal new research pathways to decipher. |
| first_indexed | 2024-12-24T04:57:53Z |
| format | Article |
| id | doaj.art-6450eb5559e54769826f2ef5c41d9dce |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-24T04:57:53Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-6450eb5559e54769826f2ef5c41d9dce2022-12-21T17:14:20ZengBMCBMC Research Notes1756-05002020-01-011211610.1186/s13104-019-4840-9Two-hybrid screening of FAM13A protein partners in lung epithelial cellsManon Ruffin0Kristin E. Thompson1Harriet Corvol2Loic Guillot3Sorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSASorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSASorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSASorbonne Université, INSERM, Centre de Recherche Saint-Antoine, CRSAAbstract Objectives Family with sequence similarity 13 member A (FAM13A) genetic variants have been associated with several chronic respiratory diseases including chronic obstructive pulmonary disease (COPD), cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF) and lung cancer. The FAM13A protein includes a RhoGTPase activating protein (RhoGAP) domain known to participate in various cellular mechanisms including cell proliferation. While intensive genomic studies have been performed to reveal its involvement in lung diseases, the biological role of FAM13A protein is still not completely elucidated. Results We therefore performed a two-hybrid screening to identify protein partners of FAM13A using a human lung cancer cDNA library. We identified several protein partners with a high confidence score. Researchers in the field of chronic lung diseases may benefit from this two-hybrid screening data which may reveal new research pathways to decipher.https://doi.org/10.1186/s13104-019-4840-9FAM13AChronic lung diseasesLung epitheliumTwo-hybrid screening |
| spellingShingle | Manon Ruffin Kristin E. Thompson Harriet Corvol Loic Guillot Two-hybrid screening of FAM13A protein partners in lung epithelial cells BMC Research Notes FAM13A Chronic lung diseases Lung epithelium Two-hybrid screening |
| title | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
| title_full | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
| title_fullStr | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
| title_full_unstemmed | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
| title_short | Two-hybrid screening of FAM13A protein partners in lung epithelial cells |
| title_sort | two hybrid screening of fam13a protein partners in lung epithelial cells |
| topic | FAM13A Chronic lung diseases Lung epithelium Two-hybrid screening |
| url | https://doi.org/10.1186/s13104-019-4840-9 |
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